LOCUS       sequence-376125-       11396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate flag-tagged ACE2 stable expressing 
            cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, 
            Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, 
            Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, 
            Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, 
            Carette JE
  TITLE     The human disease gene LYSET is essential for lysosomal enzyme 
            transport and viral infection.
  JOURNAL   Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648.
  PUBMED    36074821
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648."
COMMENT     Sequence Label: pLenti-CMV_ACE2-Flag_Hygro
FEATURES             Location/Qualifiers
     source          1..11396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    953..974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(989..994,995..1012,1013..1019)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1027..1043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1032..1054
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1051..1067
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1051..1067
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1086..1106
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1088..1106
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1134..1360
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1361..1541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1588..1713
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2206..2439
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2624..2668
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2935..3051
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3074..3377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3378..3581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3532..3552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3578..3602
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3686..3810
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3835..3840,3841..3858,3859..3865)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6301..6324
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(6333..6457)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    6488..7076
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6541..6561)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6959..6970)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7132..7631
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7167..7190)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7564..7583
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7649..8674
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7649..8674
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9048..9281
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9353..9474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9390..9409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9444..9463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9514..9649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9669..9688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9670..9688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9698..9715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9698..9714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9707..9729)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9856..10311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9943..9962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10153..10174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10337..10441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10442..10510,10511..11302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10660..10679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      901 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      961 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1021 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1081 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     1141 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     1201 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     1261 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     1321 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     1381 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1441 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1501 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     1561 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     1621 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     1681 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1741 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1801 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1861 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1921 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1981 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2041 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     2101 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     2161 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     2221 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     2281 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     2341 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     2401 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     2461 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     2521 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2581 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2641 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2701 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2761 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2821 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2881 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2941 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3001 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     3061 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3181 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3361 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3421 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3481 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     3601 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagtcca
     3661 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta
     3721 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3781 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     3841 ctttatgctt ccggctcgta taatgtgtgg attttgagtt agaccatgtc aagctcttcc
     3901 tggctccttc tcagccttgt tgctgtaact gctgctcagt ccaccattga ggaacaggcc
     3961 aagacatttt tggacaagtt taaccacgaa gccgaagacc tgttctatca aagttcactt
     4021 gcttcttgga attataacac caatattact gaagagaatg tccaaaacat gaataatgct
     4081 ggggacaaat ggtctgcctt tttaaaggaa cagtccacac ttgcccaaat gtatccacta
     4141 caagaaattc agaatctcac agtcaagctt cagctgcagg ctcttcagca aaatgggtct
     4201 tcagtgctct cagaagacaa gagcaaacgg ttgaacacaa ttctaaatac aatgagcacc
     4261 atctacagta ctggaaaagt ttgtaaccca gataatccac aagaatgctt attacttgaa
     4321 ccaggtttga atgaaataat ggcaaacagt ttagactaca atgagaggct ctgggcttgg
     4381 gaaagctgga gatctgaggt cggcaagcag ctgaggccat tatatgaaga gtatgtggtc
     4441 ttgaaaaatg agatggcaag agcaaatcat tatgaggact atggggatta ttggagagga
     4501 gactatgaag taaatggggt agatggctat gactacagcc gcggccagtt gattgaagat
     4561 gtggaacata cctttgaaga gattaaacca ttatatgaac atcttcatgc ctatgtgagg
     4621 gcaaagttga tgaatgccta tccttcctat atcagtccaa ttggatgcct ccctgctcat
     4681 ttgcttggtg atatgtgggg tagattttgg acaaatctgt actctttgac agttcccttt
     4741 ggacagaaac caaacataga tgttactgat gcaatggtgg accaggcctg ggatgcacag
     4801 agaatattca aggaggccga gaagttcttt gtatctgttg gtcttcctaa tatgactcaa
     4861 ggattctggg aaaattccat gctaacggac ccaggaaatg ttcagaaagc agtctgccat
     4921 cccacagctt gggacctggg gaagggcgac ttcaggatcc ttatgtgcac aaaggtgaca
     4981 atggacgact tcctgacagc tcatcatgag atggggcata tccagtatga tatggcatat
     5041 gctgcacaac cttttctgct aagaaatgga gctaatgaag gattccatga agctgttggg
     5101 gaaatcatgt cactttctgc agccacacct aagcatttaa aatccattgg tcttctgtca
     5161 cccgattttc aagaagacaa tgaaacagaa ataaacttcc tgctcaaaca agcactcacg
     5221 attgttggga ctctgccatt tacttacatg ttagagaagt ggaggtggat ggtctttaaa
     5281 ggggaaattc ccaaagacca gtggatgaaa aagtggtggg agatgaagcg agagatagtt
     5341 ggggtggtgg aacctgtgcc ccatgatgaa acatactgtg accccgcatc tctgttccat
     5401 gtttctaatg attactcatt cattcgatat tacacaagga ccctttacca attccagttt
     5461 caagaagcac tttgtcaagc agctaaacat gaaggccctc tgcacaaatg tgacatctca
     5521 aactctacag aagctggaca gaaactgttc aatatgctga ggcttggaaa atcagaaccc
     5581 tggaccctag cattggaaaa tgttgtagga gcaaagaaca tgaatgtaag gccactgctc
     5641 aactactttg agcccttatt tacctggctg aaagaccaga acaagaattc ttttgtggga
     5701 tggagtaccg actggagtcc atatgcagac caaagcatca aagtgaggat aagcctaaaa
     5761 tcagctcttg gagataaagc atatgaatgg aacgacaatg aaatgtacct gttccgatca
     5821 tctgttgcat atgctatgag gcagtacttt ttaaaagtaa aaaatcagat gattcttttt
     5881 ggggaggagg atgtgcgagt ggctaatttg aaaccaagaa tctcctttaa tttctttgtc
     5941 actgcaccta aaaatgtgtc tgatatcatt cctagaactg aagttgaaaa ggccatcagg
     6001 atgtcccgga gccgtatcaa tgatgctttc cgtctgaatg acaacagcct agagtttctg
     6061 gggatacagc caacacttgg acctcctaac cagccccctg tttccatatg gctgattgtt
     6121 tttggagttg tgatgggagt gatagtggtt ggcattgtca tcctgatctt cactgggatc
     6181 agagatcgga agaagaaaaa taaagcaaga agtggagaaa atccttatgc ctccatcgat
     6241 attagcaaag gagaaaataa tccaggattc caaaacactg atgatgttca gacctccttt
     6301 gattacaagg atgacgacga taagtaatcg accatagtga ctggatatgt tgtgttttac
     6361 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     6421 tttacgtttc tcgttcagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     6481 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6541 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6601 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6661 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6721 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6781 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6841 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6901 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6961 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7021 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7081 aattctgcag atatccggac tagtgatctc tcgaggttaa cgaattctac cgggtagggg
     7141 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     7201 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     7261 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     7321 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     7381 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     7441 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     7501 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     7561 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     7621 cgggcctttc gacctgcatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt
     7681 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     7741 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     7801 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     7861 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     7921 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7981 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     8041 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     8101 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     8161 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     8221 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     8281 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     8341 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     8401 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     8461 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     8521 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     8581 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     8641 acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccga acaagagctg
     8701 atttcgagaa cgcctcagcc agcaactcgc gcgagcctag caaggcaaat gcgagagaac
     8761 ggccttacgc ttggtggcac agttctcgtc cacagttcgc taagctcgct cggctgggtc
     8821 gcgggagggc cggtcgcagt gattcaggcc cttctggatt gtgttggtcc ccagggcacg
     8881 attgtcatgc ccacgcactc gggtgatctg actgatcccg cagattggag atcgccgccc
     8941 gtgcctgccg attgggtgca gatccgtcga gggcccggta cctttaagac caatgactta
     9001 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     9061 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     9121 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9181 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9241 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     9301 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     9361 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9421 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9481 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     9661 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     9721 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9781 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9841 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     9901 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     9961 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    10021 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10081 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10141 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    10201 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    10261 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    10321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    11041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    11101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    11161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    11221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatc
//
