LOCUS       sequence-376126-        8227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable 
            expressing cell line under CMVTRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, 
            Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, 
            Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, 
            Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, 
            Carette JE
  TITLE     The human disease gene LYSET is essential for lysosomal enzyme 
            transport and viral infection.
  JOURNAL   Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648.
  PUBMED    36074821
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648."
COMMENT     Sequence Label: pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro
FEATURES             Location/Qualifiers
     source          1..8227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     488..507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     741..758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    875..896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(911..916,917..934,935..941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    949..965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     954..976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     973..989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     973..989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1008..1028
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1010..1028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1056..1282
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1283..1463
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1510..1635
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2128..2361
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2546..2590
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2857..2974
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3001..3379
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3009..3027
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3045..3063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3081..3099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3117..3135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3153..3171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3189..3207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3225..3243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3288..3312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3942..4530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3995..4015)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4413..4424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4544..5043
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4579..4602)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4976..4995
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5064..5663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5064..5083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5560..5580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5801..6034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6106..6227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6143..6162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6197..6216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6267..6402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6422..6441)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6423..6441)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6451..6468)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6451..6467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6460..6482)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6609..7064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6696..6715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6906..6927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7090..7194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7195..7263,7264..8055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7413..7432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8226..8227,1..587)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
       61 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
      181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      961 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     1021 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     1081 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     1141 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     1201 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     1261 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     1321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1441 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1501 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1561 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1621 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1681 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1741 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1801 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1861 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1921 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1981 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     2041 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     2101 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     2161 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     2221 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     2281 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     2341 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     2401 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2461 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2521 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2581 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2641 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2701 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2761 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2821 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     2881 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2941 attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgagtt
     3001 ggctttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     3061 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     3121 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     3181 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     3241 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     3301 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
     3361 cacttcctac cctcgtaaac cggttcgtcg actagtccag tgtggtggaa ttctgcagat
     3421 accatgccaa agccacccga ttattcagag ctgagtgact ctttaacgct tgccgtggga
     3481 acaggaagat tttcgggacc attgcacaga gcatggagaa tgatgaactt ccgtcagcgg
     3541 atgggatgga ttggagtggg attgtatctg ttagccagtg cagcagcatt ttactatgtt
     3601 tttgaaatca gtgagactta caacaggctg gccttggaac acattcaaca gcaccctgag
     3661 gagccccttg aaggaaccac atggacacac tccttgaaag ctcaattact ctccttgcct
     3721 ttttgggtgt ggacagttat ttttctggta ccttacttac agatgttttt gttcctatac
     3781 tcttgtacaa gagctgatcc caaaacagtg ggctactgta tcatccctat atgcttggca
     3841 gttatttgca atcgccacca ggcatttgtc aaggcttcta atcagatcag cagactacaa
     3901 ctgattgaca cgtaaatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     3961 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4021 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4081 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4141 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4201 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4261 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4321 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4381 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4441 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4501 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     4561 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4621 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4681 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4741 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4801 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4861 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4921 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     4981 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5041 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5101 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5161 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5221 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5281 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5341 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5401 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5461 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5521 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5581 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5641 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     5701 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     5761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     5821 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6001 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6061 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6241 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6421 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6481 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6541 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6601 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6961 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7021 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7081 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7141 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7201 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7261 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7321 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7381 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7441 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7501 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7561 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7621 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7681 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7741 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7801 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7861 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7921 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7981 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8041 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8101 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8161 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8221 tcttctt
//
