LOCUS       sequence-376133-        9013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpa-18 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY95
FEATURES             Location/Qualifiers
     source          1..9013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4378..4398
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4420..4440
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6604..6626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6726..6745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6823..6841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6909..7013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7014..7082,7083..7874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7232..7251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8045..8633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8534..8553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8787..8804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8921..8942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8957..8962,8963..8980,8981..8987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8995..9011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9000..9013,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cacccgtttc
      181 accatatcca tttaaatgaa taaacaataa ttgcttaatg ggaaaaatgg ttgacatttt
      241 gcacttcttg attactttgt gaaactgaac tattcgaact ctctgaaaat attataaatt
      301 tcctaaaaat ttcatgcaaa atttcgaaaa aaagggcatg tttcacaatt agcatatctg
      361 tgcaaattgt attactgatg ttgcttaact ttttgatcaa caaagatatg catttaattt
      421 ttttgttctc gcgtcattta gtttttattt ttatttactt ggggtctcta aatctgcaaa
      481 agtaaaaaat gaaatattaa gcaggtgaaa aatgagtgaa atatagtttc tacccgttcc
      541 ttttagaaat taattttcac ctgtcatgat tgttctttaa aatttttaaa gtgaattttg
      601 tagcaatttt acattgattt ttgtgaattt gatgcaacat catcatatga gaaaaatttt
      661 agatttttaa gtttctattt aatttttttt tcaaaaaaaa attactgttt caaaattagc
      721 atatttgaca aaattatgtt gcgggtgtga tacgtttgta ttatacaatt ttcctaaaca
      781 aaaaaaataa ggactttgtc atatttagaa aaaaggaaaa cgttacgaac tgacagggaa
      841 taaattacat atttagtact ttctaaaaac caggtgaaac atatttttag agctgtcgtc
      901 attgagtttt gttttcgaaa atgacgtttt gagttcactt ttgagatttt ctccttctcc
      961 ttaacaagat tttctaacct tccattaaat atttctagaa ttttaatttt ctttttctac
     1021 cgtgcccaaa tattaccatt tttatgtcac attttaccaa tattcaaatt taaaaattac
     1081 gttttgtttt gtttccatct cttccttatt tacatatctt ttagttcatt agtgtttaat
     1141 cagaagtata tacgcttcag tcaccagaca ggcaacatca tgatcctcaa cgcttttcga
     1201 tgaaaatgac tcatttccat tttccactac ttcacattgt ggtttaaaaa cgaaaaacat
     1261 tttttaatca ttctcttctc ttcataaatt ttcaggtaaa cttttttatt tgattcctta
     1321 attgaccttt tgacaaatct ctaactcatc tactagttta aaataaaaca ttgttggtct
     1381 aataagaatc tctaataaga atttgcaaat cttaatagaa atccgataac caaaataacc
     1441 aattttgatc acattttttg aaaatctcta attacaacct agttccctct ttccttttca
     1501 aaagttcaac tagcctgctc actggctctc catatttttt tcccgtatac ttgatattta
     1561 atctcaatat ccttcttttt tcaacatcta atcctctact cttttcattc tctctattca
     1621 tttcatattc tttgagtttt tcttttcaat tgcacctatg ggtagattag attgcgattt
     1681 acattttcct ctccggtaag cttatatgct caaactacga gcttggttta tttttattat
     1741 tacacttgct cgctttctta tttcaagtgc cacctggttg gattcttatc caactttttt
     1801 aatttttgaa aatgagtatg tatgtatatt tgataatctt gtttagctaa aataattaaa
     1861 atcgcagcgt gattattgca gcaaaattaa taaaaatggc atgctcatat gtgcaacacg
     1921 aaacagtaca ttttagtttg agttttttaa agttttagtt tatattctcg tcaatcgcga
     1981 tattttttat cagacaaagt tcttgcgaat cagaacaaaa tatccgtgac gtttatattt
     2041 ttaaattcca agtttccatc ttttcttttt taaaatacaa aattacttta tttttacatt
     2101 ttagttagaa gagaaccgct taaaggtggt gtagtcgaat tttttttaat tgccttatta
     2161 gactcaagat tgtctgaaaa cgccaaattt cataagaaac ttcttgaaaa cttttcagaa
     2221 aaaagttatt acggctcaaa aaatggccta aaattagtta aaattggaaa tttgaccaac
     2281 ttatctttgt cgcagcggct ggaaacaatt tttttgaaat caccgtcaaa ttttgagtac
     2341 acaagttgct taccttgcgt tttaagcttc attaaagtat ttaaaagtcg atgtacggcg
     2401 agttttggct caaaaaaatt aaaaatctcg ccgttcatcg atttttaagt actttaatga
     2461 agtttaaaac gcaaggtaag caacttgtac actcaaaatt tgacggtgat ttttaaaaaa
     2521 attgtttcca gccgctgcga cattgacaag ttagtcaaat ttccgacttt aactaatttt
     2581 agatcatttt ttgagccgtc ataacttttt tctaaaaagt tttcaagaag tttcactctg
     2641 aaatttggtg ttttcagaca attttgagtc taataaagca ataaaaaaaa ttcgactaca
     2701 ccacctttaa tgtgaaacta ctgtgtaaat ttggtagagc atatatgtat gagcatcgtg
     2761 aaacagtaaa cattaaaacc taatttaact tacgttaaat acgaaaagta tatcatacta
     2821 cttgaagcgt ggctgctagt tggaaaatta gctcattttg aacatatttc gctgaaggcc
     2881 gcttgaataa aaagctaaaa agctatgagc caaaacaagt caaaaataaa aatttaattt
     2941 cgatattttc gagataaaaa cattttgaaa tgttataaat taaataaact tatcaaattt
     3001 ttttaaatta aaatcggttt attaatcgat tttactgaaa aattgaacat gaaagtatta
     3061 caatttcgat gatcttttct gttgggtcaa tttgctcttg atttcaagtt ttatattata
     3121 tttttatttt aaaattaatg ctaaaaatag tatttttagg gcgtttctaa catattttcc
     3181 cgttatatta agcgaatagt actatggaat taacagacat atttatatga gcaccatgaa
     3241 acagtaaaca gtgtgttaaa aatgctcgaa actaagaaaa ttgttttttt ttacattggg
     3301 aggtttcaca tctaacaaaa ataaatttaa aacccaataa tttttgtata aaactacata
     3361 gcaataaatt tattacttgg gaaaaaacac cagaaatttt aatcttcggt gttttagaaa
     3421 tataaaataa aaatttttat ttttctaacc ggaaatgttt ctattttatt ttgtgaaatt
     3481 aattacaaaa tttagagctt gttaggtaaa taaacttcag aagttcagaa agttcaaaat
     3541 aatacagctt tttttaaaaa ttaaaaattt caagtttctt gaagatgcct agacttcgtt
     3601 ttttattcat gtcacgaaaa agtctgaatt ttcagaaaaa ctatgtttta tctaaaaatt
     3661 gcacaaaaaa atttttctgt tttatttgac tataaatttc agattaaatt aattcaaatt
     3721 tacaacttag acaatttaga taatttactg tttcatgttt tttatatagg tactgtgttg
     3781 ttttgttttt ctattcattg cttggtcttt tttttccatg aaatcacata tctcacactt
     3841 tcaaaaatca ggttcccaca agatcattac tccgtcaaag tccacaaaac aagtgcaaca
     3901 cacaaattac tcatacttct gatttagaat ttttgaaaac agaaaaacag aaaataatca
     3961 gttctcatta ggaaaggtac ataaataagc cgaaagaagc ttgaaaattg aattttcata
     4021 tgactcttca cgtgatttta gtgcaaaact ttccaaaaac aaaactccaa atgttttaaa
     4081 ttccaactga tttcaaagat tttattgaat caattaaatt cttcaaaata aattaaaatt
     4141 ctgaaacaga gttttttttt tcaactttcc aatttctcca ttttaaacct gatatgcgcc
     4201 ttattaaatt cacaccatag aaaactcaaa atttcagtcc aaaattacca ctagaatttc
     4261 aaacccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
     4321 aacattttca ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gaccgctcca
     4381 aagaagaagc gcaaggttcc agtcgagaag atgactgccc caaaaaagaa acgtaaggtc
     4441 ccgcggatgt cagagcttat taaggagaat atgcatatga aactgtacat ggaaggaacc
     4501 gtggacaacc atcactttaa gtgcacttcg gagggggaag gcaaaccgta cgagggcacc
     4561 cagaccatgc gaatcaaggt ggtcgaggga ggcccactgc ccttcgcctt cgacattctg
     4621 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca ggctactagc
     4681 ttcctgtacg gcagcaagac ttttattaat cacacccagg gcatcccgga cttcttcaaa
     4741 cagtcgttcc ctgagggctt cacatgggag cgtgtgacca catacgaaga cggtggcgtt
     4801 ctcaccgcta cccaggacac cagcctccag gacggttgcc tcatatataa tgtggtaagt
     4861 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagaagat ccggggagta
     4921 aacttcacat ccaatggtcc ggttatgcag aagaaaacac tcggctggga ggccttcacc
     4981 gagacgctgt atccggctga cggaggcctg gaaggtcgaa acgacatggc cctgaagctc
     5041 gtcggcggta gccatctcat cgcaaacgct aagaccacat atagatccaa gaaaccggta
     5101 agtttaaaca tgattttact aactaactaa tctgatttaa attttcaggc taagaacctt
     5161 aaaatgccgg gagtctacta tgttgactac agactggaac ggatcaagga ggccaacaac
     5221 gagacctacg tcgagcagca cgaggttgca gttgccagat actgcgacct cccgagcaaa
     5281 ctgggtcaca agcttaatcc taggataccg gtgggtgaag acccaccaaa gccatctgcc
     5341 aagggagcca agaaggccgc caagaccgtt acgaagccaa aggacggaaa gaagagacgt
     5401 catgcccgta aggaatcata ctccgtctac atctaccgtg tcctcaagca agttcatcca
     5461 gacactggag tttcctccaa agccatgtct atcatgaact cttttgtcaa cgatgtcttc
     5521 gagcgtattg ctgctgaagc atcccgtctt gctcactaca acaagcgttc cacaatctca
     5581 tcccgcgaaa ttcagaccgc tgtccgtctg atccttccag gagagcttgc caagcacgcc
     5641 gtgtctgagg gaaccaaggc cgttaccaag tacacttcca gcaagtagga attccaactg
     5701 agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca
     5761 tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc
     5821 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
     5881 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     5941 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     6001 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     6061 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     6121 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     6181 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     6241 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     6301 ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac
     6361 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     6421 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     6481 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     6541 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6601 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6661 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6721 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6781 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     6841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8161 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8941 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9001 gcggataaca att
//
