LOCUS       sequence-376135-        8131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable 
            expressing cell line under CMVTRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, 
            Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, 
            Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, 
            Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, 
            Carette JE
  TITLE     The human disease gene LYSET is essential for lysosomal enzyme 
            transport and viral infection.
  JOURNAL   Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648.
  PUBMED    36074821
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648."
COMMENT     Sequence Label: pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro
FEATURES             Location/Qualifiers
     source          1..8131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      111..699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     600..619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     853..870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    987..1008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1023..1028,1029..1046,1047..1053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1061..1077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1066..1088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1085..1101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1085..1101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1120..1140
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1122..1140
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1168..1394
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1395..1575
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1622..1747
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2240..2473
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2658..2702
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2969..3086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3113..3491
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3157..3175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3193..3211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3229..3247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3265..3283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3301..3319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3337..3355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3400..3424
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3958..4546
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4011..4031)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4429..4440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4560..5059
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4595..4618)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4992..5011
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5080..5679
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5080..5099)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5576..5596
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5817..6050
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6122..6243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6159..6178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6213..6232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6283..6418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6438..6457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6439..6457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6467..6484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6467..6483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6476..6498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6625..7080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6712..6731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6922..6943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7106..7210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7211..7279,7280..8071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7429..7448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
       61 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      961 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1021 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1081 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     1141 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     1201 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     1261 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     1321 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     1381 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     1441 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     1501 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1561 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     1621 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     1681 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     1741 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     1801 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1861 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1921 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1981 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     2041 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     2101 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     2161 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     2221 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     2281 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     2341 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     2401 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     2461 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     2521 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     2581 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     2641 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     2701 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     2761 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2821 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2881 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2941 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     3001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     3061 aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag ttggctttac
     3121 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
     3181 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
     3241 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
     3301 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
     3361 taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
     3421 gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt accacttcct
     3481 accctcgtaa accggttcgt cgactagtcc agtgtggtgg aattctgcag ataccatgat
     3541 gaacttccgt cagcggatgg gatggattgg agtgggattg tatctgttag ccagtgcagc
     3601 agcattttac tatgtttttg aaatcagtga gacttacaac aggctggcct tggaacacat
     3661 tcaacagcac cctgaggagc cccttgaagg aaccacatgg acacactcct tgaaagctca
     3721 attactctcc ttgccttttt gggtgtggac agttattttt ctggtacctt acttacagat
     3781 gtttttgttc ctatactctt gtacaagagc tgatcccaaa acagtgggct actgtatcat
     3841 ccctatatgc ttggcagtta tttgcaatcg ccaccaggca tttgtcaagg cttctaatca
     3901 gatcagcaga ctacaactga ttgacacgta aatccagcac agtggcggcc gctcgacaat
     3961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4321 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4381 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4441 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4501 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     4561 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4621 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4681 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4741 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4801 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     4861 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     4921 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     4981 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5041 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     5101 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5161 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5221 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5281 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5341 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5401 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5461 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5521 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5581 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5641 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     5701 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     5761 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5821 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6061 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6121 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6241 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6421 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6601 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6841 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6961 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7021 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7081 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7141 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7201 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7261 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7321 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7381 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7441 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7501 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7561 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7621 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7681 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7741 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7801 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7861 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7921 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7981 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8041 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8101 ttagattgat ttaaaacttc atttttaatt t
//
