LOCUS       sequence-376136-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking mRNA of arsenic responsive gene 
            cluster as CRISPR RNA (site 2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY168
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..630)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     535..554
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        646..701
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    682..700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             719..730
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(737..763,764..766,767..3253,3254..3256,3257..4843)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4907..4928
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      4931..4974
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(4978..4996)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5061..5165
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5166..5234,5235..6026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5384..5403)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6056..6150
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6642..6864
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6912..7862
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     8124..8141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8165..8222)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    8223..8243
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(8350..9063)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             9070..9081
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(9114..9163)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             complement(9366..9377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(10375..10493)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      complement(10551..10578)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        10594..10628
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             10646..11482
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             complement(11626..11637)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtattaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
       61 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      121 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      181 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      241 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      301 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      361 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      421 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      481 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      541 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      601 cactttactt ttatctaatc ttgacatcat taattcctaa tttttgttga cactctatcg
      661 ttgatagagt tattttacca ctccctatca gtgatagaga aaagaattga aaagatctaa
      721 agaggagaaa ggatctatgg ataagaaata ctcaataggc ttagctatcg gcacaaatag
      781 cgtcggatgg gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct
      841 gggaaataca gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag
      901 tggagagaca gcggaagcga ctcgcctcaa acggacagct cgtagaaggt atacacgtcg
      961 gaagaatcgt atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga
     1021 tagtttcttt catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg
     1081 tcatcctatt tttggaaata tagtagatga agttgcttat catgagaaat atccaactat
     1141 ctatcatctg cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta
     1201 tttggcctta gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa
     1261 tcctgataat agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt
     1321 atttgaagaa aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg
     1381 attgagtaaa tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa
     1441 tggcttattt gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa
     1501 ttttgatttg gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt
     1561 agataattta ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa
     1621 tttatcagat gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc
     1681 tcccctatca gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt
     1741 aaaagcttta gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc
     1801 aaaaaacgga tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt
     1861 tatcaaacca attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg
     1921 tgaagatttg ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca
     1981 cttgggtgag ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga
     2041 caatcgtgag aagattgaaa aaatcttgac ttttcgtatt ccttattatg ttggtccatt
     2101 ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc
     2161 atggaatttt gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat
     2221 gacaaacttt gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta
     2281 tgagtatttt acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg
     2341 aaaaccagca tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac
     2401 aaatcgaaaa gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt
     2461 tgatagtgtt gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca
     2521 tgatttgcta aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat
     2581 cttagaggat attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag
     2641 acttaaaaca tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg
     2701 ttatactggt tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc
     2761 tggcaaaaca atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca
     2821 gctgatccat gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg
     2881 acaaggcgat agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa
     2941 aggtatttta cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa
     3001 gccagaaaat atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa
     3061 aaattcgcga gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat
     3121 tcttaaagag catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta
     3181 tctccaaaat ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga
     3241 ttatgatgtc gatgccattg ttccacaaag tttccttaaa gacgattcaa tagacaataa
     3301 ggtcttaacg cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt
     3361 agtcaaaaag atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg
     3421 taagtttgat aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg
     3481 ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt
     3541 ggatagtcgc atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt
     3601 gattacctta aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt
     3661 acgtgagatt aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac
     3721 tgctttgatt aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt
     3781 ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa
     3841 atatttcttt tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg
     3901 agagattcgc aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga
     3961 taaagggcga gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt
     4021 caagaaaaca gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa
     4081 ttcggacaag cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga
     4141 tagtccaacg gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa
     4201 gaagttaaaa tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga
     4261 aaaaaatccg attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat
     4321 cattaaacta cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc
     4381 tagtgccgga gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt
     4441 tttatattta gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa
     4501 acaattgttt gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga
     4561 attttctaag cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa
     4621 caaacataga gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt
     4681 gacgaatctt ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg
     4741 atatacgtct acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct
     4801 ttatgaaaca cgcattgatt tgagtcagct aggaggtgac taatactaga gccaattatt
     4861 gaaggggagc gggaaaccgc tccccttttt ttgtttctgg tctcccctct agaagcggcc
     4921 gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc
     4981 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5041 gtggcaccat gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5101 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5161 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5221 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5281 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5341 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5401 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5461 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5521 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5581 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5641 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5701 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5761 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5821 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5881 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5941 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6001 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttacgagctc gcttggactc
     6061 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     6121 tgccgccggg cgttttttat tggtgagaat ccaagcacta gggacagtaa gacgggtaag
     6181 cctgttgatg ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg
     6241 gataatccga agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag
     6301 tcagatagca ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt
     6361 ttcttgtatt atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt
     6421 acccccattc actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg
     6481 actcaggtgc ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga
     6541 gggtgctact taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt
     6601 gcgacatcct tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg
     6661 gatctattct ttttatcttt ttttattctt tctttattct ataaattata accacttgaa
     6721 tataaacaaa aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta
     6781 caagttttcc agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga
     6841 catgtaatta tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc
     6901 aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct
     6961 atgacttaac ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca
     7021 cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc
     7081 agatgatgaa catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc
     7141 tgatgacgag aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt
     7201 ggacaaacta tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat
     7261 tgccttatct tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt
     7321 ttgaaaacaa atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga
     7381 aaactcacaa ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg
     7441 aaaataacta ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag
     7501 atttaaacac ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata
     7561 cgttgatttt ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca
     7621 accagataaa aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac
     7681 ataacggact aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt
     7741 ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga tctcaatggt tcgttctcat
     7801 ggctcacgca aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct
     7861 gaggttctta tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga
     7921 acaactgttc accgttacat atcaaaggga aaactgtcca tatgcacaga tgaaaacggt
     7981 gtaaaaaaga tagatacatc agagctttta cgagtttttg gtgcattcaa agctgttcac
     8041 catgaacaga tcgacaatgt aacctcgaga ttaccgcctt tgagtgagct gataccgctc
     8101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa
     8161 gtaatctttt cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg
     8221 gactgcagcg gccgctacta gtagggagac cagaaacaaa aaaaggcccc ccgttaggga
     8281 ggccttcaat aattggctct agtattatta aactgatgca gcgtagtttt cgtcgtttgc
     8341 agcaggcctt ttgtacagtt catccatacc atgcgtgatg cccgctgcgg ttacgaactc
     8401 cagcagaacc atatgatcgc gtttctcgtt cggatcttta gacagaacgc tttgcgtgct
     8461 cagatagtga ttgtctggca gcagaacagg accatcaccg attggagtgt tttgctggta
     8521 gtgatcagcc agctgcacgc tgccatcctc cacgttgtgg cgaattttaa aattcgcttt
     8581 aatgccattt ttttgtttat cggcggtgat gtaaacattg tggctgttaa aattgtattc
     8641 cagcttatgg cccaggatat tgccgtcttc tttaaagtca atgcctttca gctcaatgcg
     8701 gtttaccagg gtatcgcctt caaatttcac ttccgcacgc gttttgtacg tgccgtcatc
     8761 cttaaaggaa atcgtgcgtt cctgcacata gccttccggc atggcggact tgaagaagtc
     8821 atgctgcttc atatggtccg gataacgagc aaagcactga acaccataag tcagcgtcgt
     8881 taccagagtc ggccaaggta ccggcagttt accagtagta cagatgaact tcagcgtcag
     8941 tttaccatta gttgcgtcac cttcaccctc gccacgcacg gaaaacttat gaccgttgac
     9001 atcaccatcc agttccacca gaatagggac gacaccagtg aacagctctt cgcctttacg
     9061 catatggtat ttctcctctt taatctctag ttaaacaaaa ttatttgtag aggctgtttc
     9121 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctctcta gtatttgcgt
     9181 tcaggattgt cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat
     9241 ttataaagat ctgattgaag aatcaacagc aacatgccag gatgagttag cgaattacac
     9301 taaccggtat ctcattacaa agtgaactct agtagggaga ccagaaacaa aaaaaggccg
     9361 cgttagcggc cttcaataat tggctctagt attaatttgc tgctttccat tgagcctgtt
     9421 tctctgcgcg acgttcgcgg cggcgtgtgc gtgcattcat ttgcaaactg agttgcagca
     9481 actctttttc ctgctgcatc tgaaactcac gtaaatccag cggcagtgtt ggaagcgagg
     9541 tggtttctga aacggcgata gcgtcttcag gcggacgccg tttaaaggga tcaataatga
     9601 tgtcatcaag cggataatcg ctggtgccgt ggcgatacac tgaacgttcc accacgtttt
     9661 tcaattcacg aatatttccc ggccaacgat aattcagcaa tgtttctctg gcgcgctccg
     9721 taaaccccgg gaacagaggc agcttgattt cccgacacat ctggatggca aagtattctg
     9781 ccatcaacat tatgtcgctt tcgcgctcgc gcagtggtgg cagttgtaca acatcaaaag
     9841 ccagtcggtc gagcaggtca gcgcgaaaag tgccttcatt gaccatcgcc gggagatcgg
     9901 cattcgtcgc gcataccaac cgcacattca cctgcaatgg ttggctgccg ccaacgcgct
     9961 ccagttcacc gtactcaatc acgcgcaata atttctcctg caccatcatc ggtgccgtag
    10021 cgagttcatc aagaaatagc gtaccgccgt cggcacgttc aaatctccct ggatgacgtt
    10081 tttgcgcacc ggtaaacgcc cccgcttcgt gaccaaacag ttcggaatcc agcagatttt
    10141 catttaacgc cgcgcagtta agggaaataa acggcccttg ccaacgggag gagagataat
    10201 gcaggcggct ggcaatcagc tctttaccgg tgccgcgttc gccgatgatg agcaccggtt
    10261 tgtccagcgg tgcgagatgc gaaacctgtt ccagcacttc gagaaagctg ttcgcctcac
    10321 caagtaaatt atctttgtat tctgccatct agtactttcc tgtgtgactc tagttacgac
    10381 cagtctaaaa agcgcctcaa ttcgcgacct tctcgttact gacaggaaaa tgggccattg
    10441 gcaaccaggg aaagatgaac gtgatgatgt tcacaatttg ctgaattgtg gtgatgtgat
    10501 gctcaccgca ttcctgctcc agcggcgtaa aggtctctct agtatataaa cgcagaaagg
    10561 cccacccgaa ggtgagccag tgtgatacta gagtttacag ctagctcagt cctaggtatt
    10621 atgctagcta ctagagggag cccaaatgac cgtattacat agtgtggatt tttttccgtc
    10681 cggtaacgcg tccgtggcga tagaaccccg gctcccgcag gcggattttc ctgaacatca
    10741 tcatgatttt catgaaattg tgattgtcga acatggcacg ggtattcatg tgtttaatgg
    10801 gcagccctat accatcaccg gtggcacggt ctgtttcgta cgcgatcatg atcggcatct
    10861 gtatgaacat accgataatc tgtgtctgac caatgtgctg tatcgctcgc cggatcgatt
    10921 tcagtttctc gccgggctga atcagttgct gccacaagag ctggatgggc agtatccgtc
    10981 tcactggcgc gttaaccaca gcgtattgca gcaggtgcga cagctggttg cacagatgga
    11041 acagcaggaa ggggaaaatg atttaccctc gaccgccagt cgcgagatct tgtttatgca
    11101 attactgctc ttgctgcgta aaagcagttt gcaggagaac ctggaaaaca gcgcatcacg
    11161 tctcaacttg cttctggcct ggctggagga ccattttgcc gatgaggtga attgggatgc
    11221 cgtggcggat caattttctc tttcactgcg tacgctacat cggcagctta agcagcaaac
    11281 gggactgacg cctcagcgat acctgaaccg cctgcgactg atgaaagccc gacatctgct
    11341 acgccacagc gaggccagcg ttactgacat cgcctatcgc tgtggattca gcgacagtaa
    11401 ccacttttcg acgctttttc gccgagagtt taactggtca ccgcgtgata ttcgccaggg
    11461 acgggatggc tttctgcaat aatactagct cggtaccaaa gacgaacaat aagacgctga
    11521 aaagcgtctt ttttcgtttt ggtccctcta gtattaattt tgtcttcatg catgaagaca
    11581 actaggctct agtagggaga ccagaaacaa aaaaaggccg cgttagcggc cttcaataat
    11641 tggctcta
//
