LOCUS       sequence-37622-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA
  TITLE     A new DAF-16 isoform regulates longevity.
  JOURNAL   Nature. 2010 Jul 22. 466(7305):498-502.
  PUBMED    20613724
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 22. 466(7305):498-502."
COMMENT     Sequence Label: L4440-daf-16a
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7..23
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..462)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..462)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(519..538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(520..538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(562..579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccctcgag gtcgacggta tcgataagct tctggacatc tacctgtata cacaccagtg
       61 gccagtcatc tgccattaca atttcatcaa ttgacacttc ttcaacaaca accgccgtcc
      121 tcattcactc ccgattcttc ctcatcctca acatcgtcgt ctttggctga aattcccgaa
      181 gacgttatga tggagatgct ggtagatcag ggaactgatg catcgtcatc cgcctccacg
      241 tccacctcat ctgtttcgag attcggagcg gacacgttca tgaatacacc ggatgatgtg
      301 atgatgaatg atgatatgga accgattcct cgtgatcggt gcaatacgtg gccaatgcgt
      361 aggccgcaac tcgaaccacc actcaactcg agtcccatta ttcatgaaca aattcctgaa
      421 gaagatgcta gccatggaac cggtggatcc actagttcta gagcggccgc caccgcggtg
      481 gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat
      541 ttcgataagc caggttgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaa
//
