LOCUS       sequence-376259-       12209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking mRNA of zntA as CRISPR RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY315
FEATURES             Location/Qualifiers
     source          1..12209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(125..229)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(316..359)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    362..383
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(join(447..2033,2034..2036,2037..4523,4524..4526,
                     4527..4553))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             4560..4571
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        4589..4644
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    4590..4608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4660..5286
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4736..4755)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5301..5312
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5456..6292)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        complement(6310..6344)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      6360..6387
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        6445..6563
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             7561..7572
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_RNA        7775..7824
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             7857..7868
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7875..8588
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    8695..8715
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      8716..8773
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(8797..8814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9076..10026)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      10074..10296
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(10788..10882)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(10912..11703,11704..11772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11535..11554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11773..11877)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12001..12023)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12123..12142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
       61 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttccca
      241 tggtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      301 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      361 ggaattcgcg gccgcttcta gaggggagac cagaaacaaa aaaaggggag cggtttcccg
      421 ctccccttca ataattggct ctagtattag tcacctccta gctgactcaa atcaatgcgt
      481 gtttcataaa gaccagtgat ggattgatgg ataagagtgg catctaaaac ttcttttgta
      541 gacgtatatc gtttacgatc aattgttgta tcaaaatatt taaaagcagc gggagctcca
      601 agattcgtca acgtaaataa atgaataata ttttctgctt gttcacgtat tggtttgtct
      661 ctatgtttgt tatatgcact aagaacttta tctaaattgg catctgctaa aataacacgc
      721 ttagaaaatt cactgatttg ctcaataatc tcatctaaat aatgcttatg ctgctccaca
      781 aacaattgtt tttgttcgtt atcttctgga ctacccttca acttttcata atgactagct
      841 aaatataaaa aattcacata tttgcttggc agagccagct catttccttt ttgtaattct
      901 ccggcactag ccagcatccg tttacgaccg ttttctaact caaaaagact atatttaggt
      961 agtttaatga ttaagtcttt tttaacttcc ttatatcctt tagcttctaa aaagtcaatc
     1021 ggattttttt caaaggaact tctttccata attgtgatcc ctagtaactc tttaacggat
     1081 tttaacttct tcgatttccc tttttccacc ttagcaacca ctaggactga ataagctacc
     1141 gttggactat caaaaccacc atattttttt ggatcccagt cttttttacg agcaataagc
     1201 ttgtccgaat ttctttttgg taaaattgac tccttggaga atccgcctgt ctgtacttct
     1261 gttttcttga caatattgac ttggggcatg gacaatactt tgcgcactgt ggcaaaatct
     1321 cgccctttat cccagacaat ttctccagtt tccccattag tttcgattag agggcgtttg
     1381 cgaatctctc catttgcaag tgtaatttct gttttgaaga agttcatgat attagagtaa
     1441 aagaaatatt ttgcggttgc tttgcctatt tcttgctcag acttagcaat cattttacga
     1501 acatcataaa ctttataatc accatagaca aactccgatt caagttttgg atatttctta
     1561 atcaaagcag ttccaacgac ggcatttaga tacgcatcat gggcatgatg gtaattgtta
     1621 atctcacgta ctttatagaa ttggaaatct tttcggaagt cagaaactaa tttagatttt
     1681 aaggtaatca ctttaacctc tcgaataagt ttatcatttt catcgtattt agtattcatg
     1741 cgactatcca aaatttgtgc cacatgctta gtgatttggc gagtttcaac caattggcgt
     1801 ttgataaaac cagctttatc aagttcactc aaacctccac gttcagcttt cgttaaatta
     1861 tcaaacttac gttgagtgat taacttggcg tttagaagtt gtctccaata gtttttcatc
     1921 tttttgacta cttcttcact tggaacgtta tccgatttac cacgattttt atcagaacgc
     1981 gttaagacct tattgtctat tgaatcgtct ttaaggaaac tttgtggaac aatggcatcg
     2041 acatcataat cacttaaacg attaatatct aattcttggt ccacatacat gtctcttcca
     2101 ttttggagat aatagagata gagcttttca ttttgcaatt gagtattttc aacaggatgc
     2161 tctttaagaa tctgacttcc taattctttg ataccttctt cgattcgttt catacgctct
     2221 cgcgaatttt tctggccctt ttgagttgtc tgattttcac gtgccatttc aataacgata
     2281 ttttctggct tatgccgccc cattactttg accaattcat caacaacttt tacagtctgt
     2341 aaaatacctt ttttaatagc agggctacca gctaaatttg caatatgttc atgtaaacta
     2401 tcgccttgtc cagacacttg tgctttttga atgtcttctt taaatgtcaa actatcatca
     2461 tggatcagct gcataaaatt gcgattggca aaaccatctg atttcaaaaa atctaatatt
     2521 gttttgccag attgcttatc cctaatacca ttaatcaatt ttcgagacaa acgtccccaa
     2581 ccagtataac ggcgacgttt aagctgtttc atcaccttat catcaaagag gtgagcatat
     2641 gttttaagtc tttcctcaat catctcccta tcttcaaata aggtcaatgt taaaacaata
     2701 tcctctaaga tatcttcatt ttcttcatta tccaaaaaat ctttatcttt aataattttt
     2761 agcaaatcat ggtaggtacc taatgaagca ttaaatctat cttcaactcc tgaaatttca
     2821 acactatcaa aacattctat ttttttgaaa taatcttctt ttaattgctt aacggttact
     2881 tttcgatttg ttttgaagag taaatcaaca atggctttct tctgttcacc tgaaagaaat
     2941 gctggttttc gcattccttc agtaacatat ttgacctttg tcaattcgtt ataaaccgta
     3001 aaatactcat aaagcaaact atgttttggt agtacttttt catttggaag atttttatca
     3061 aagtttgtca tgcgttcaat aaatgattga gctgaagcac ctttatcgac aacttcttca
     3121 aaattccatg gggtaattgt ttcttcagac ttccgagtca tccatgcaaa acgactattg
     3181 ccacgcgcca atggaccaac ataataagga atacgaaaag tcaagatttt ttcaatcttc
     3241 tcacgattgt cttttaaaaa tggataaaag tcttcttgtc ttctcaaaat agcatgcagc
     3301 tcacccaagt gaatttgatg gggaatagag ccgttgtcaa aggtccgttg cttgcgcagc
     3361 aaatcttcac gatttagttt caccaataat tcctcagtac catccatttt ttctaaaatt
     3421 ggtttgataa atttataaaa ttcttcttgg ctagctcccc catcaatata acctgcatat
     3481 ccgttttttg attgatcaaa aaagatttct ttatactttt ctggaagttg ttgtcgaact
     3541 aaagctttta aaagagtcaa gtcttgatga tgttcatcgt agcgtttaat cattgaagct
     3601 gataggggag ccttagttat ttcagtattt actcttagga tatctgaaag taaaatagca
     3661 tctgataaat tcttagctgc caaaaacaaa tcagcatatt gatctccaat ttgcgccaat
     3721 aaattatcta aatcatcatc gtaagtatct tttgaaagct gtaatttagc atcttctgcc
     3781 aaatcaaaat ttgatttaaa attaggggtc aaacccaatg acaaagcaat gagattccca
     3841 aataagccat ttttcttctc accggggagc tgagcaatga gattttctaa tcgtcttgat
     3901 ttactcaatc gtgcagaaag aatcgcttta gcatctactc cacttgcgtt aatagggttt
     3961 tcttcaaata attgattgta ggtttgtacc aactggataa atagtttgtc cacatcacta
     4021 ttatcaggat ttaaatctcc ctcaatcaaa aaatgaccac gaaacttaat catatgcgct
     4081 aaggccaaat agattaagcg caaatccgct ttatcagtag aatctaccaa tttttttcgc
     4141 agatgataga tagttggata tttctcatga taagcaactt catctactat atttccaaaa
     4201 ataggatgac gttcatgctt cttgtcttct tccaccaaaa aagactcttc aagtcgatga
     4261 aagaaactat catctacttt cgccatctca tttgaaaaaa tctcctgtag ataacaaata
     4321 cgattcttcc gacgtgtata ccttctacga gctgtccgtt tgaggcgagt cgcttccgct
     4381 gtctctccac tgtcaaataa aagagcccct ataagatttt ttttgatact gtggcggtct
     4441 gtatttccca gaaccttgaa ctttttagac ggaaccttat attcatcagt gatcaccgcc
     4501 catccgacgc tatttgtgcc gatagctaag cctattgagt atttcttatc catagatcct
     4561 ttctcctctt tagatctttt caattctttt ctctatcact gatagggagt ggtaaaataa
     4621 ctctatcaac gatagagtgt caacaaaaat taggaattaa tgatgtcaag attagataaa
     4681 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     4741 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     4801 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     4861 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     4921 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     4981 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     5041 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     5101 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     5161 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     5221 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     5281 tcttaatact agagccaatt attgaaggcc gctaacgcgg cctttttttg tttctggtct
     5341 ccctactaga gcctagttgt cttcatgcat gaagacaaaa ttaatactag agggaccaaa
     5401 acgaaaaaag acgcttttca gcgtcttatt gttcgtcttt ggtaccgagc tagtattatt
     5461 gcagaaagcc atcccgtccc tggcgaatat cacgcggtga ccagttaaac tctcggcgaa
     5521 aaagcgtcga aaagtggtta ctgtcgctga atccacagcg ataggcgatg tcagtaacgc
     5581 tggcctcgct gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc
     5641 gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag
     5701 aaaattgatc cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg
     5761 ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac
     5821 gcagcaagag cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat
     5881 tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt
     5941 ggttaacgcg ccagtgagac ggatactgcc catccagctc ttgtggcagc aactgattca
     6001 gcccggcgag aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat
     6061 tatcggtatg ttcatacaga tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg
     6121 tgatggtata gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa
     6181 tttcatgaaa atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg
     6241 ccacggacgc gttaccggac ggaaaaaaat ccacactatg taatacggtc atttgggctc
     6301 cctctagtag ctagcataat acctaggact gagctagctg taaactctag tatcacactg
     6361 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagaccttta cgccgctgga
     6421 gcaggaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt
     6481 catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattg
     6541 aggcgctttt tagactggtc gtaactagag tcacacagga aagtactaga tggcagaata
     6601 caaagataat ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca
     6661 tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct
     6721 gattgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa
     6781 ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc
     6841 gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct
     6901 atttcttgat gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat
     6961 tgagtacggt gaactggagc gcgttggcgg cagccaacca ttgcaggtga atgtgcggtt
     7021 ggtatgcgcg acgaatgccg atctcccggc gatggtcaat gaaggcactt ttcgcgctga
     7081 cctgctcgac cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag
     7141 cgacataatg ttgatggcag aatactttgc catccagatg tgtcgggaaa tcaagctgcc
     7201 tctgttcccg gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg
     7261 aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga
     7321 ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat
     7381 cgccgtttca gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat
     7441 gcagcaggaa aaagagttgc tgcaactcag tttgcaaatg aatgcacgca cacgccgccg
     7501 cgaacgtcgc gcagagaaac aggctcaatg gaaagcagca aattaatact agagccaatt
     7561 attgaaggcc gctaacgcgg cctttttttg tttctggtct ccctactaga ggattctgaa
     7621 actgatcgaa gcggttagtg taattcgcta actcatcctg gcatgttgct gttgattctt
     7681 caatcagatc tttataaatc aaaaagataa aaaattggca cgcaaattgt attaacagtt
     7741 cagcaggaca atcctgaacg caaatactag agagctgtca ccggatgtgc tttccggtct
     7801 gatgagtccg tgaggacgaa acagcctcta caaataattt tgtttaacta gagattaaag
     7861 aggagaaata ccatatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
     7921 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
     7981 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
     8041 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
     8101 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
     8161 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     8221 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
     8281 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
     8341 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
     8401 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     8461 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
     8521 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     8581 tgtacaaaag gcctgctgca aacgacgaaa actacgctgc atcagtttaa taatactaga
     8641 gccaattatt gaaggcctcc ctaacggggg gccttttttt gtttctggtc tccctactag
     8701 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     8761 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     8821 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct cgaggttaca
     8881 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
     8941 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
     9001 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     9061 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     9121 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
     9181 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     9241 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     9301 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     9361 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     9421 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     9481 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     9541 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     9601 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     9661 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     9721 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     9781 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     9841 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     9901 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     9961 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
    10021 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
    10081 tcaatgataa ttacatgtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
    10141 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
    10201 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
    10261 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
    10321 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
    10381 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
    10441 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
    10501 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
    10561 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
    10621 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
    10681 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
    10741 gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata
    10801 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
    10861 actggatcta tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gttaccaatg
    10921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11521 ggcgtcaaya cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11881 atttcccnna nwrgtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    11941 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    12001 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    12061 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    12121 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    12181 atgcgtaagg agaaaatacc gcatcagga
//
