LOCUS       sequence-376267-        8902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-6 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY16
FEATURES             Location/Qualifiers
     source          1..8902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4267..4287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4309..4329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6493..6515)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6615..6634
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6712..6730)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6798..6902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6903..6971,6972..7763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7121..7140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7934..8522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8423..8442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8676..8693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8810..8831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8846..8851,8852..8869,8870..8876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8884..8900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8889..8902,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg gaggaggaaa
      181 tcctagaatt atgaaaaaga gagcaaaaag aagatgttcg tgaagtgacg tgaagtttaa
      241 gaaaagaaga ttggacgatg aatgggcaaa aaaacggaaa gaaatgttcc ttctgaatat
      301 ggaactttgc aggaatattt ttttaaaaat cacaattaaa aacaagtatt tccagctaac
      361 attttcattc gtggaaaaaa caataacggt gatggaatca atacgtttaa aatggaacaa
      421 caatcttttt aatactgtat tttgccaatt tctcttgcgt accaatttct ctgatttaac
      481 agcgactgaa aatgttcaat tattagaagt gcataaatag ataaagcata taactggaca
      541 attttcaata tcaccggtgt taaaatttgg ggtgcaatac taataagaaa aaaaaggtac
      601 ctgaagtatt ttcagtaaca attgcgaatt ctttcccaaa ctttgtctta acatcatgca
      661 tgcttaattc aactaggtat gaatatttca atgcacattt gcatacaatt cggcaggtga
      721 actcatgaga aagttcattt tgtatgtgaa ggtcatgttg agtagacaca agtgttgaat
      781 tcaattcagt cttacctttt tgatcaatat ttttgaggtt ttaattccaa tttaaaaaaa
      841 tcaatcaaaa cccctcactg tgaatagcta atttaccatt cacatttcac tttgcgtctt
      901 caaatagcca gactcccacc aaactaaacg attctgtgca cgatctgcac aattttaaat
      961 ccttccgttc ttctgttcat tctcttgtag acttctgttg tatgccttca ggtctttgat
     1021 ttctttctca cgatgtctga atgatctagt cacaattggc ttcttcgtca gaagttcctc
     1081 tattggtggc tcacatgggc cacaaagtgc ccgttgaaac acatttttgc attttcgatt
     1141 atcccgtacc ttttccttcc tctttatatg ttctgtaact ttttggtttc actacttttc
     1201 gaagactaag gtgttcgatc gtttctcaca tttatttaac catatatgga agatgactcg
     1261 aaccaacaaa agaaatcaac gccctctcag tgaagatttt atctttcaat ttttattcaa
     1321 aaaatattta atcagaatat ctattcatat cactttcatg ttgattattg aaaccttaca
     1381 attggatagg aaatgtcaca cgacatatga tattccagtt gtagaaaatc aaagttatgt
     1441 gaaaaaacaa gcacgttttt tgaatgaaga cgcttgactt ctgatgacat atcaagaatt
     1501 aaagattttc caaaatgcaa cccatgtttg attcaaggat atgttgttgt ttccagaaac
     1561 acaacataat gcaaattcac tcaatacttt ctttctaaag taaatctttg attaactttc
     1621 agaattgtaa gaaaaaccaa acaatttcag aattaatcca gaagcaggaa actgcataaa
     1681 aaaacaacaa aaaaaaataa tccttctgac aaatgttgac aaaaattatc gtaaatttac
     1741 aattttctaa aaatcttcta gatttctttt ccctatgacg gtcgcattaa agtactgaaa
     1801 taacataata aaactttaat aataaatttc ttattagaaa attacgattt tttcattttg
     1861 acacttcaca gctaactgaa atttttagca cttaattgtc tatgtttttc caacttcaaa
     1921 gatcaattta accttcgaaa aacacctgat cacctgaaac tcaatgtttc aatttcattg
     1981 ttcaattatt aatgatgtag tacaaattta acaaacacaa aattttccca tcttcacgtc
     2041 acttctgctt acataaccta aaatttcagg acgcttttat ttttgaaaga tccggaaagt
     2101 gaaatactag ttctgggatt ttgcaagtcc tgcaaaatga aaaaaaaatc tatggcttca
     2161 tcttggtttt gaaaaattcc aaaaaaaagt gattccatga atcagatcag gaagtactgt
     2221 tcttttattt tttaaaataa catatataaa tcttcttttt attttatttt cttaaaaagt
     2281 taatccaaaa aaaaagaatt gtttttctcc agggaacggc agtaaatcaa atgacgcagt
     2341 ggatggatca gtgttgggat gttcctggaa ttgtccaccc tataaccaaa acaaaaagct
     2401 gattggaata ttgcttgaaa aatctgaaac taagatgatt ttctgaaaaa ccgaagaagt
     2461 ttagaagatc atatatttcc cgaataacgc tataatattt attgaactaa ctttaaaata
     2521 ttctttagga aagtataaat cgcgagatat aaaattctta aatcctagtt caattgtgat
     2581 aatgtctaaa aaatgactaa taagaaaata tgagaattgt tctatatgcg tcattgacat
     2641 tttcaaaaga agccttatta gagcagacgg attagatgag tactcataat ttaaaaaaac
     2701 gataactgaa aatcatacga tctatcaaag tttcctaaat gttaaccaaa gagtatcgag
     2761 aattggcaac aagattcagc acgaaaactt cctgattttc tccgaacttc tgatttcatc
     2821 tcacattagc tggaatttaa agctgaccga aaaaatagtt taacttaaat gaatattgcg
     2881 caagtaaatg tttatttatt ttaaagtgag acagcttcta attattttgt ttgttttcgg
     2941 tcgatgcacc atgttttgca ttgggttcag tggatgtatt gatgtggaat aaaagttaat
     3001 ctactgtcag attgttgact gggttttttt aaaataccat aagatcctac acttaacgaa
     3061 acagtgacag ggcacagttt tgaaatgtgt ggtgcatatg tgaattgatg attttagtga
     3121 cccaattgcc tttaatgtta atattggttc aagtgaacgt ttgttctcgt tcaaggtgtc
     3181 tcataaacat gcttagaaaa tctcaaaaat gattttccca caaagtccat aaaaattaat
     3241 taatttgttc tttgactcat tttcactgca catttggttc cttctctcac gttctcttac
     3301 ttttttgtat tttttgaaat ctgaaaattg gagtatgagc tgcgatacaa gtcactttga
     3361 atctcgaaat gaatccatca aatttccttg tttgatgtca tcaatcttat ttgctcttct
     3421 tcaatgtttc ggtgaaaagt cagaaatatt gcaattctca atattctcta tggtaactcc
     3481 gtgtttttgt tttgtctggc ttttgactca ggttaggatc cacctataaa actaactttt
     3541 ttaccagtta aacatgacgt caatcttcct ttctcatccc caaattcgga ttagccccca
     3601 ccaaaaccag caaaaagacg gtgaagggcg ggaaagagcc gttggagaaa ggaatacctt
     3661 caacagcttg actccgccca cttcttcgtc cgcttctcct cccctcaaaa aagcgagtcg
     3721 ggggtctcat tcggaggctc ttttctaagc caatatcatc ttctttttgc tcttttgtag
     3781 tagtactaat agataataat attaatcttt ttattttcaa ccaacttttc acctggtact
     3841 ccccctcatc caaattcaga catgttccac gccccacgtt accagatgat atttatgaaa
     3901 agttgaaaat gtacttcata caacctgacc ttcgcagacg tatagctgaa tctctatgtt
     3961 tttctgttca acgtttttta tttagttatt actcattttt gttttatatg aaaaacaagg
     4021 tataataggt ataatatttg cttcctgctt taaggattct tatctcataa aatcctgaaa
     4081 tccattcctg ccttcttatc ttgttttcag tttttaatca ttattttgaa aacaatatga
     4141 ttattccaga atcccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
     4201 ataacataga acattttcag gaggaccctt gcttggaggg taccgagctc agaaaaaatg
     4261 accgctccaa agaagaagcg caaggttcca gtcgagaaga tgactgcccc aaaaaagaaa
     4321 cgtaaggtcc cgcggatgtc agagcttatt aaggagaata tgcatatgaa actgtacatg
     4381 gaaggaaccg tggacaacca tcactttaag tgcacttcgg agggggaagg caaaccgtac
     4441 gagggcaccc agaccatgcg aatcaaggtg gtcgagggag gcccactgcc cttcgccttc
     4501 gacattctgg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     4561 gctactagct tcctgtacgg cagcaagact tttattaatc acacccaggg catcccggac
     4621 ttcttcaaac agtcgttccc tgagggcttc acatgggagc gtgtgaccac atacgaagac
     4681 ggtggcgttc tcaccgctac ccaggacacc agcctccagg acggttgcct catatataat
     4741 gtggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagaagatc
     4801 cggggagtaa acttcacatc caatggtccg gttatgcaga agaaaacact cggctgggag
     4861 gccttcaccg agacgctgta tccggctgac ggaggcctgg aaggtcgaaa cgacatggcc
     4921 ctgaagctcg tcggcggtag ccatctcatc gcaaacgcta agaccacata tagatccaag
     4981 aaaccggtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcaggct
     5041 aagaacctta aaatgccggg agtctactat gttgactaca gactggaacg gatcaaggag
     5101 gccaacaacg agacctacgt cgagcagcac gaggttgcag ttgccagata ctgcgacctc
     5161 ccgagcaaac tgggtcacaa gcttaatcct aggataccgg tgggtgaaga cccaccaaag
     5221 ccatctgcca agggagccaa gaaggccgcc aagaccgtta cgaagccaaa ggacggaaag
     5281 aagagacgtc atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt cctcaagcaa
     5341 gttcatccag acactggagt ttcctccaaa gccatgtcta tcatgaactc ttttgtcaac
     5401 gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg ctcactacaa caagcgttcc
     5461 acaatctcat cccgcgaaat tcagaccgct gtccgtctga tccttccagg agagcttgcc
     5521 aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt acacttccag caagtaggaa
     5581 ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg
     5641 ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt
     5701 ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac
     5761 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     5821 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     5881 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     5941 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct
     6001 gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     6061 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     6121 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     6181 tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat
     6241 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     6301 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     6361 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     6421 aacataactg tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga
     6481 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     6541 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     6601 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     6661 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga
     6721 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6781 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6841 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6901 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6961 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7021 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7081 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7141 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7201 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7261 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7321 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7381 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7441 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7501 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7561 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7621 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7681 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7741 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7801 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7861 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7921 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7981 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8041 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8101 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8161 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8221 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8281 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8341 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8401 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8521 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8581 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8641 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8701 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8761 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8881 gaattgtgag cggataacaa tt
//
