LOCUS       sequence-376268-        9584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter driving panneuronal nuclear GCaMP6s expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY12
FEATURES             Location/Qualifiers
     source          1..9584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4621..4641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4666..4686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4708..4728
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4753..4770
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(4810..4818,4819..4833)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             4861..4917
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(7175..7197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7297..7316
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7394..7412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7480..7584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7585..7653,7654..8445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7803..7822)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8616..9204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9105..9124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9358..9375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9492..9513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9528..9533,9534..9551,9552..9558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9566..9582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9571..9584,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gagttttgac
      181 tggctttcaa aaactgagtg attactaatt atttttgaaa aaattaacag ttgtgatatg
      241 atatttagac atttttgcaa tatataattt gtttgaaaaa aaaacatttt ttaatggtct
      301 tcgaagctat aatagttttt cgtaaaattt taaataaaac agctgacaga tatccacatt
      361 caataattga aagtatgcag ttctcttttt ttttgtcaaa tagaaaaaaa gtaacagatg
      421 gtcagatata atctaggtta atctgactct ggtcatcgat gaactcattt tcatcataat
      481 cattcttctt tcaaatggtc tgttcggaat tcgaatattc aataaatgga tatcttatta
      541 gatgctccgg atttattcca ccaaacttcc aagacgtaag ttcaatttaa acaaaattac
      601 tatagcttga aaaaatgaga aaaaacgagc agataaaaaa ataacagaaa aaatccataa
      661 aaatgacgag ggttccgtgc gaaaatcgtc attgtttttg gcttgcatca cacttttccg
      721 cgagtcagcc acactgtttt atcgattatt atttttctga tttcagaaac cggaaactta
      781 caactgagat gctttcattt tgattttttt aaatttagtt ttgatttcag cgatttgttt
      841 gaatataatt atgcatgagt tggcttttgc atttttagat aaataagttc acatgctcgg
      901 acaaattgtg tggtttaatc tgtgaaagtt ttgaatcttc tgtttgaaaa aaaaaacatt
      961 tttaatttgt atatacgagt gaattggtta ggccctggtt acgttttcca gcgaattcat
     1021 tcaaatttct tattttttct attttttcaa ttaaataatt tagttctcta tcacatgtgt
     1081 atgatcaaaa tttcattcct aatcgtaagc aaatttaggc tcatccgtag tataattttg
     1141 ttatgaaggg cttcacagta aaagctcaaa ctccaacatt tagcaactag caaccaacaa
     1201 aaaaccaaag aaatgtcagt tttgaaaagt cgttatttct ttgtcttctc acaattcaca
     1261 catcatcttc tcgttttcct ccgttttttt gtcgtgcttc cttcttttct ttacacattt
     1321 ttctaacctt tttttaagtc tatcctctcc taatcctttg tactctctaa tcagttttgc
     1381 tggttgtgct gcacaatttt tatcagattt atgcgcgaaa caaaagcctc tcgcggagag
     1441 ctccgccaaa tccggtcttc ttttttcctt gcctatcgga tttcacaaac attcagtttg
     1501 tcacttgacg atgtgttgga ggcattacta ctaggaagtg ttattcatat tttttattcg
     1561 tttctttttg gtgactaaac gaaatattcc gaacgttttc ctgatttcgg gcggcggaaa
     1621 cgaagaagac caagtgagtt ctttgaaaat ctttattaga gttatttgcg acaatttgcc
     1681 actttttttt taatgtttaa tgaatctgta acgaaattaa taccaagggt gtccaacaga
     1741 actatctgag cggttattta atttttagat agtatatttc ttccgatcgt aacatacata
     1801 ttttcaagca tagtactgtg atttaagtta cagttttcca atcagtcttg attttaataa
     1861 aaacttttca actgcaaaaa tgcctctgaa gtatttttct atatttttat agttgaataa
     1921 ttttttgatt ggcacaaaga ccgccgagat atgagccgtt aaagtaaagc tgcatggatt
     1981 caatatggtt tgtatttgat tggagtcact tacatggaaa tatttgtatt taaatctcat
     2041 attttaaaac aagacaaaca ccaaattaaa tttttattaa tctgaacatt tttcaatcga
     2101 tttggtaaac aaattattag atgtttccct caaaaattgt aacaattcct aaattgtggc
     2161 acttataata acatcgtcct gaattctcaa aaaaaaccga atgctaagag agatttcgtt
     2221 taactgggac ctgtaactga attttcaaat attggcagtt tttgataaac tgggagggaa
     2281 atcaaaaacc aaaactacag tacccctcgt gttataataa attgtcattc actccttttt
     2341 tgttcccaac atttgctccc atctcctgac gagaaagaac aattttatga gcacgggaac
     2401 ccgtgggcgt ccatccgttc ctcctgtgca atttcagccc ccttcctcta cattctctgt
     2461 gttcccgttt ttatcgtttt atgggtatcg ggaacggaca gatcgcttcc atttgcttct
     2521 attccgtcct cacaagacgt cccactaact gattttcttc tgatttcttt tcactgaaaa
     2581 tgggaaagaa agaaaaagac gatgaatcga cgacttcttc tcaggagaag gtgaagaaga
     2641 aggataagga gaaagttcat ctaaggctga aagataaagt aaaaaaactt agaaaatcta
     2701 agtcgattgc agtatcttct ccagctgatt atatgacttg tcagtgtgaa ccatgcaaaa
     2761 aagtagctct cgaggtaata atttttaaac tcaatacgta gttaagtttg aactcctttt
     2821 aatttactta tcatctaatt taccgtcaca atagtacttc cattagcaat tattttaaaa
     2881 ttacatgcat agcttcaatg ttcgtttttt tgcgttctca tatcgtatgt tgtctgcctc
     2941 tcgaatactg actgtccgac tagaatgtcc cggtaggatt actgtacggt cactgggaat
     3001 aaactgtttc tgaaatcaat ctgaaattcc gtagatcttc ttggttcaat ttcttattcc
     3061 tcaatctttg gacaacgtca ccttcagagt cgtcgttgag tatcccatca ttaactagtc
     3121 aatctttctc tctttttcat ttcccccaac ttttctgcat attttttgac gacgacgacc
     3181 tcgacggcaa cgagaataca tttatctatt ttttagtata gcatatatac attttctact
     3241 tttctctaac ccatttcaga cgtatagatt ttggttttcg aatgcaactt tgagattttt
     3301 caaagatctt cagatgggag cagtggactg tctatttgat attcttaaaa tgtactctat
     3361 aaaagatgaa actgtaatga ccaattccct acataaatat ttttaactaa ttggtcggat
     3421 atttggggat cagagaagta cttgtttttg cgatattaat aatgatatat ttttctagta
     3481 aaaaaatgtt aagcgatttg tgaataatta agttggcttg aaaaagattt tttgatagct
     3541 atgtattttg ttaatttaaa tcagatttta aaactttatt attgactatt tatatatatt
     3601 atatattata tattttcatg gtttaatcaa aatctattaa attatttcta agatctctaa
     3661 atatcgaatt ctctaaaaag cttcatcaac aaaagcattt caattatcta acacaatgag
     3721 aataattcaa gaactgaaat agaaagaaat ctagaaaaaa aagagagtga ggtgccctat
     3781 taaatcggtg ctctcctctc taacagaagc gcctacacgc acccaagaga gctctttttc
     3841 tttctttttt cggagcacac atacacgcat acactcctca gaagtgtata gagaaaatgg
     3901 gagaagaagc gaaagaagac gaagaagcgg ttgtgatgta tgcatcgggc gtttctatcc
     3961 ctctccgtga gcaacgagct agtcaaccca tcgatccatt tttgtgagca cacacagaga
     4021 gagactcaaa cacactcaca gcttcacact gagctccaag agctctgtag agagaagcca
     4081 gagtatgctt tcctcgattt tcctaacgtc ttcttcttct gcttttttct ttttcccaac
     4141 gagttgtagt ccactaactt caatttttcc tccagagaaa gagttttaga gagacagctg
     4201 ataaatacac tagagaaata aaaagaatga taaaaatttg ctattttaat cagttccctc
     4261 tcgtttctct aagttttgtt gttgggttgg gaaattaaaa ttggaaaatg gaaataacgt
     4321 ttttttctgt ctaatcttga attctccaga catttcttat ttcctttttt gttgcaagtt
     4381 tttcttcctt ctctaatttt taatcaaaga tatctcgcta tgaactttga atttttgata
     4441 ttttgattct ataaatacat aaagtaatct aaatgctctt ttaaaataaa tctacagtag
     4501 ccctattttc agcccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
     4561 ataacataga acattttcag gaggaccctt ggagggtacc ggtcagaaaa aatgactgct
     4621 ccaaaaaaaa aacgcaaagt tccatcatcc gaaaagatga ccgccccaaa gaagaagcgc
     4681 aaggtccctg tcgccgagaa gactgccccg aagaaaaagc gcaaggtccc atctgccgcc
     4741 gctatgggat cccaccacca ccatcatcac ggaatggcct ccatgactgg aggacaacaa
     4801 atgggacgcg acctctacga cgacgacgac aaggacctcg ccacaatggt cgactcctcc
     4861 cgtcgcaaat ggaataagac cggacacgcc gtccgtgcca tcggtcgtct ctcctccctc
     4921 gagaacgtct acatcaaggc cgacaagcaa aagaacggaa tcaaggtaag tttaaacata
     4981 tacatactaa ctaaccctga ttatttaaat tttcaggcca acttccacat ccgtcacaac
     5041 atcgaggacg gaggagtcca actcgcctac cactaccaac aaaacacccc aatcggagac
     5101 ggaccagtcc tcctcccaga caaccactac ctctccgtcc aatccaagct ctcaaaggac
     5161 ccaaacgaga aacgcgatca catggtcctt cttgaatttg tcactgccgc cggcattaca
     5221 ctgggaatgg atgaactcta taagggagga accggaggat ccatggtctc caaaggagag
     5281 gagctcttca ccggagtcgt cccaatcctc gtcgagctcg acggagacgt caacggtcac
     5341 aagttctccg tctccggaga gggagaggga gacgccacct acggaaagct caccctcaag
     5401 ttcatctgca ccactggaaa gctcccagtc ccatggccaa ccctcgtcac caccctcacc
     5461 tacggagtcc aatgcttctc ccgttaccca ggtaagttta aagagatcga tactaacaaa
     5521 ccatacatat ttaaattttc agaccacatg aagcaacacg acttcttcaa gtccgccatg
     5581 ccagagggat acatccaaga gcgtaccatc ttcttcaagg acgacggaaa ctacaagacc
     5641 cgtgccgagg tcaaattcga gggagacacc ctcgtcaacc gcatcgagct caagggaatc
     5701 gacttcaagg aggacggaaa catcctcgga cacaagctcg aatacaacct cccagaccaa
     5761 ctcaccgagg agcaaatcgc tgaattcaaa gaggccttct cactttttga caaggacgga
     5821 gacggaacca tcaccactaa ggtaagttta aagaagattt tactaactaa ctaatctgat
     5881 ttaaattttc aggagctcgg aaccgtcatg cgttccctcg gacaaaaccc aaccgaggcc
     5941 gagctccaag acatgatcaa cgaggtcgac gccgacggag acggaaccat cgacttccca
     6001 gagttcctca ccatgatggc ccgcaagatg aagtaccgcg acactgaaga ggagattcgc
     6061 gaagccttcg gagtcttcga caaggacgga aacggataca tctccgccgc cgagctccgt
     6121 cacgtcatga ccaacctcgg agagaagctc accgacgagg aggtcgacga gatgatccgc
     6181 gaggccgaca tcgacggaga cggacaagtc aactacgagg aattcgtcca aatgatgacc
     6241 gccaaggcct aggataccgg aattccaact gagcgccggt cgctaccatt accaacttgt
     6301 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     6361 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     6421 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     6481 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     6541 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     6601 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     6661 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     6721 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     6781 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     6841 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     6901 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     6961 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     7021 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     7081 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     7141 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7201 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7261 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7321 gagtgcacca tatgcggtgc gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7381 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7441 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7501 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7561 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7801 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8101 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     9421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
