LOCUS       sequence-376269-        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  acr-5 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY62
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4525..4545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4567..4587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6751..6773)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6873..6892
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6970..6988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7056..7160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7161..7229,7230..8021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7379..7398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8192..8780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8681..8700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8934..8951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9068..9089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9104..9109,9110..9127,9128..9134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9142..9158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9147..9160,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcactagtg atctgctcta
      181 ataatggctg gttcagcaca tcaaaacgag tacgactttt cgcaacgacg gcattcactt
      241 atcaacatgt tcaaggcgtt taaattgtaa acaatcttgt gatgcagcat tgctccaatg
      301 ataatttggg tatttcatag ggtgttattt tgcatctgtt tatctatgaa gaagctgaaa
      361 actggaatta tcattagagc aagttcgcaa gcagaccagc acacaaaata atgcaacaga
      421 atttggttcc caaagagctc aataaagttt acttttcaca aatgcttcaa tttccgtttc
      481 ttccatttaa attgttctga tgttatagct tctcgcactt tccctccatt tcaatttccc
      541 gcctattttc taccctcaaa agttcctctc atagttttct tattgatgag gaacttttgg
      601 gcattagcaa aattgtgccc tttgaattaa gttaaaatga attaaataat cttttattaa
      661 agcgaattcg catcatattt taaatgcgcg gtattaataa aaataggttc gtcgatcaat
      721 aggtgttttc atattatcca aatacacaat tgggcaattt gtaaaacttg aagaatgcgg
      781 caacgagttc tgtgcaagtc tttaccttta atgcttccgt catcgttagc aaaaactgtg
      841 tcatgaaaga caagtgtata agattattca aatacaattt tcttatggag tttttctagc
      901 ttttaaggat ttccaaatag aatgatcatt ccttgaataa taattctcta ttttatcaat
      961 attttgtttt tttttctatt ttagaaaggg cggagagtag tgtgtagtgg cggtttcacg
     1021 ctctgatgat ctcatgccat tgttcacctt ttccaaattg ttttaggttt ttcggagtac
     1081 ttcttttctt tcttttccct cttcattttc accctctgtt tgagcatgag tgctctttat
     1141 tctttggtga tggaataaaa agaggtgcca tcaaattcag aattttgtaa aatgttgaac
     1201 tttagagttt ttagtattcg gttagtgtga tgtttgtttt attttcaacc ctaacaaatc
     1261 tcagttttct ggaaaagttg tgcatttaca atgaatatat tgttttctgc atttcgtctg
     1321 ttcttagatt attcagtgac tttggaaatt taagctaatt cttgttgagc catttgacat
     1381 aatttgtata gaaaacaaaa atgtatactt tacaccgcga tttagatacg gcactgtgcg
     1441 gaaactagca aacaattcag agccgacttc cataaggttg ccaaaaaaat tttagtaatt
     1501 tatttaattc aataattaaa tgtatttatc acgaaaataa aatgaaaaca tttgttgttc
     1561 aaaattatat attttcagaa agaaaaaaca agtttttaaa atttaattac tacttctcat
     1621 tatgagtctc tcaaaaagct caacatttca gttagaataa aaattaaaaa atatcaatga
     1681 aagtgtcccg tctaattcca tttttcactc cccacactgt tcaacgtcaa aacacattgt
     1741 cattcagtca ttttggaaaa agcaccccga gcgcagaagc agtaggaaaa acgagtctcg
     1801 atgggggtag gttgaacgag caaaaaaaac caattcggct aattgaactc gactttttct
     1861 cccaatttta ctctcaccaa tcgtgatgcg tatctaaatg gatacgcaaa gaaaggaaaa
     1921 gaaagagctt tttattttca gatccaccca cggacacgtt tttggtagaa ctattacatc
     1981 actttttctt agttttttct gtgagcttga tgagaactgt ggtgtatttt ttgtagataa
     2041 aacaaaattt aaacctactt cataagttca cacacctacc ttatgtcagc ctagaaagtt
     2101 accacgatga tggcagaaag ttgcctcttt gcctctaaat gccgagtagt ttcaaaaaat
     2161 gttgacattt gatttggaat ttaagagtcc tctctgaaaa tgtgtaattc tgaaatttta
     2221 gtgcccattt caaaaaaata acatctgctc agcaaattgt atttcatcct ctctttattt
     2281 tttgttaccc cgtcttactt cctctctttt ttatttattg ttttttcttg tggtcctaat
     2341 aataaaaatg aaaagagaag ctgttgtgct cttccacaac aatgtgctct tttcaatatt
     2401 ctaaaatggc aatggaattg gcaattgttg gagggttaaa attgccagac aaaagtcatc
     2461 ttctctatgt ttgttcttca tcttctttta cctctattcc ttatccttct caataacatt
     2521 ctgaaactga cgatttaatg agtatacttc cgtttatgaa tgtatcatga taatttcatg
     2581 tctgaaaaag aacggagaaa tgagaatgca tattctcaac ttgaacgtat agccaagact
     2641 tatgcctatt ttcaatctgt ttagaccctg acaaacattt gaccacacag gagtgtttgg
     2701 gacaacaacc atatttttga aaacattttt gaatgagaaa tgctatcttc tgtgggaagt
     2761 tttaaagcaa gctgatttct ttatatagcc caaaataatt attttttaat agattatgca
     2821 aattaaaata tggagcttga tttgctgaag tgtctatttt gatttgattt gatttgattt
     2881 gatttattac gattggaatc gtgatggaat aaaagcagta tagtataaag acagaaaaaa
     2941 taaatacaca atacatagga agaataatca aaaagttacg aataaagtac aaacaagaaa
     3001 agcaattatc acctgcaaaa gttgattaat tggaacatta aagtgaacat gaaataggct
     3061 atttgggggt gtgaggagag tgggtgaatg atgggtaata agaggtgcca atataagatt
     3121 cgtcatgcta atttttcata gaactttaga ccctatttgt gaaaaagtgt cttcttagtt
     3181 tggttttgca tttcccccag atgaacttgg ttttggttct ggttttgttg tttttatcga
     3241 ttttgaaaaa ttggtccgtg tcaatgtcaa ctttatttaa ctttaagctt taaagaataa
     3301 acttatcaaa ataaatcaaa cggtactttt tttttgaaat ttttattcaa aattcaatct
     3361 tttcaagagc aaactgtaat tttttttgtc attcctatcg caaataaacg taaaattcag
     3421 ttaatttaaa atgtgagaaa tgaggtaaca actactatag aaaaataatg aaatgttcaa
     3481 gcgatgagca attaaaattt acgctcaaaa attaaccatg atgagaaaaa aattctgctc
     3541 ttcaagttat acaagactta tgagtgttaa ctttatactt caaaaaataa attgtattga
     3601 aacacctttt ttagagattt tagattatta gattgagaac tgtacaaaaa aacgagtgtt
     3661 ccaagataca tgagaatgat attttaaccc gaaacaatga aacattcaat catacatatt
     3721 ctcatccttt tcaactcgtc aacattccac gccttgtctc acttttttct atttcccgtc
     3781 cactgggatt ttgatttccc gcccgtttcg tctcggcatt ttttttttga aagaggttac
     3841 tctctcatct ttgacaccat cttccataaa acacgagaaa tgaataatgg aatcgaggga
     3901 ggttcttgtt tgtttttttt tgtgtgtttt tgttttgcat tttactcata ttttagaact
     3961 tcgaaatctg atcatgactc gttttgcgag aaccattgct ttaaaatatt tttattttct
     4021 tgaggataac tgtttttttg aaaaaaaaaa catggaatct ttggaagtat gctgtaaatt
     4081 gaggaagtct taaaatgttt caacttccat atgaaaaatt aaatataata cagttttgtt
     4141 tccgttacaa aatccaagac gtcttctcac aaaaaatcgc ctaaattttc atttaaaaat
     4201 gtgtctttgc aaaagttacc agtcctgtca aatactcgca acaaatatcc aagaataact
     4261 gactgatctg atttttgcag ttagtttttc ctcttcaaac tcaaaatctg tttttatttg
     4321 aaccattcaa tagtttaaac cccatttgaa tacgttttct catctttcct cgtcaccagt
     4381 ttcaatgctt taaaaacaat tttcagcatg gatccccggg attggccaaa ggacccaaag
     4441 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggagggta
     4501 ccgagctcag aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg
     4561 actgccccaa aaaagaaacg taaggtcccg cggatgtcag agcttattaa ggagaatatg
     4621 catatgaaac tgtacatgga aggaaccgtg gacaaccatc actttaagtg cacttcggag
     4681 ggggaaggca aaccgtacga gggcacccag accatgcgaa tcaaggtggt cgagggaggc
     4741 ccactgccct tcgccttcga cattctggta agtttaaaca tatatatact aactaaccct
     4801 gattatttaa attttcaggc tactagcttc ctgtacggca gcaagacttt tattaatcac
     4861 acccagggca tcccggactt cttcaaacag tcgttccctg agggcttcac atgggagcgt
     4921 gtgaccacat acgaagacgg tggcgttctc accgctaccc aggacaccag cctccaggac
     4981 ggttgcctca tatataatgt ggtaagttta aacagttcgg tactaactaa ccatacatat
     5041 ttaaattttc agaagatccg gggagtaaac ttcacatcca atggtccggt tatgcagaag
     5101 aaaacactcg gctgggaggc cttcaccgag acgctgtatc cggctgacgg aggcctggaa
     5161 ggtcgaaacg acatggccct gaagctcgtc ggcggtagcc atctcatcgc aaacgctaag
     5221 accacatata gatccaagaa accggtaagt ttaaacatga ttttactaac taactaatct
     5281 gatttaaatt ttcaggctaa gaaccttaaa atgccgggag tctactatgt tgactacaga
     5341 ctggaacgga tcaaggaggc caacaacgag acctacgtcg agcagcacga ggttgcagtt
     5401 gccagatact gcgacctccc gagcaaactg ggtcacaagc ttaatcctag gataccggtg
     5461 ggtgaagacc caccaaagcc atctgccaag ggagccaaga aggccgccaa gaccgttacg
     5521 aagccaaagg acggaaagaa gagacgtcat gcccgtaagg aatcatactc cgtctacatc
     5581 taccgtgtcc tcaagcaagt tcatccagac actggagttt cctccaaagc catgtctatc
     5641 atgaactctt ttgtcaacga tgtcttcgag cgtattgctg ctgaagcatc ccgtcttgct
     5701 cactacaaca agcgttccac aatctcatcc cgcgaaattc agaccgctgt ccgtctgatc
     5761 cttccaggag agcttgccaa gcacgccgtg tctgagggaa ccaaggccgt taccaagtac
     5821 acttccagca agtaggaatt ccaactgagc gccggtcgct accattacca acttgtctgg
     5881 tgtcaaaaat aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta
     5941 acgagtaata tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt
     6001 actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt
     6061 atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     6121 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     6181 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     6241 cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa
     6301 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     6361 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     6421 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc
     6481 cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat
     6541 gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt
     6601 agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa
     6661 tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg
     6721 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6781 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6841 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6901 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6961 gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7021 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9061 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt
//
