LOCUS       sequence-376270-        8060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking p2a-EGFP into PTPRO locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA
FEATURES             Location/Qualifiers
     source          1..8060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1042..1098
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1099..1101,1102..1104,1105..1818)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1099..1101,1102..1104,1105..1815)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1144..1165)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1405..1424)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1752..1773
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1852..1885
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1907..2383
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2106..2125)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        2444..2931
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2479..2502)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2864..2883
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        2943..2990
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3009..3812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3072..3091)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3682..3701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3844..3861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3850..4074
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4131..4164
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5213..5232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5214..5232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5239..5256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5239..5255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5248..5270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5396..5851)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5533..5554)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5745..5764
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5878..5982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5983..6051,6052..6843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6201..6220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7014..7602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7503..7522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7756..7773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7890..7911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7926..7931,7932..7949,7950..7956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7964..7980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7969..7991
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7988..8004
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7988..8004
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8025..8043
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttggcctagt ggttgatgtc ctttacttat ttgtctattt gtatatgtta ctgatttgaa
       61 tgactttatc acaaaaataa aggcatcaat attctgtcat aattctagaa aatatttttc
      121 tttcccagtt tgtggtttac cctgtgtttt attcttttta actttttaaa atatacagta
      181 gattgagatt tttatttgta atgtattcta ctgctcttaa ttttagaaat tcctttccta
      241 aaggaatcag agagaaatta accaatattt tcctctgtat ttttaaagac aatttattaa
      301 attacctgga atttgctttg gtgtatgata atgggatatc cttttttttt caaaatagcc
      361 ccatgatttg attgaatatt catttccttt agcattaata atgatttaat gtacagcatg
      421 gtgaacatag ttcataatac cgtattgtat acttgaaatt tgctaggaaa gtagatctta
      481 agtgttctca ccacacacac acacacacaa atatatatac atacatatat atgtatatat
      541 acacacacat atatataaac atatatataa tctggggtga tgaatgtgtt aactagcttg
      601 attgtggtaa tcatttcata acatatatgt atatcaagtc atcacattgt acaccttaaa
      661 tatatacaat ttttattata ctgcaataaa gttaggggaa aaaaattcca ctgttttttt
      721 aaaaaagcca tgatgttcta agctctgtta ttaatcaaaa tacttgtaca gttgattctc
      781 ttggattttc tagttataaa actgtgtctc tgtaaataat gaaaatggtt tcctttaaca
      841 ccagatcttg atcattaaaa ttgtcatagt ataaaatatt atatctttgc acatgtctgc
      901 tctgaaacct gtttttgtct tttgttccag gagcagtaca tttttatcca tcagtgtgtg
      961 caactgatgt ggatgaagaa gaagcagcag ttctgcatca gtgatgtcat atacgagaat
     1021 gttagcaagt ccggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc
     1081 gaagagaatc ctggaccgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1141 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1201 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1261 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1321 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1381 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1441 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1501 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1561 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1621 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1681 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1741 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1801 gacgagctgt acaagtaatg agaattccga tcatattcaa taacccttaa tataacttcg
     1861 tataatgtat gctatacgaa gttatagcgc tgctcgagag atctacgggt ggcatccctg
     1921 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
     1981 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
     2041 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
     2101 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
     2161 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
     2221 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
     2281 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
     2341 gctgggatta caggcgtgaa ccactgctcc cttccctgtc ctttaggtct gaagaggagt
     2401 ttacgtccag ccacgcgtag gatctcgacc tcgaaattct accgggtagg ggaggcgctt
     2461 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     2521 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     2581 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     2641 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     2701 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     2761 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     2821 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     2881 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     2941 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     3001 actaaaccat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     3061 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3121 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3181 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3241 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3301 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3361 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3421 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3481 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3541 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3601 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3661 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3721 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3781 ccttctatcg ccttcttgac gagttcttct gaggggatca attctctaga gctcgctgat
     3841 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3901 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3961 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4021 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4081 aggcggaaag aaccagctgg ggctcgacta gagcttgcgg aacccttaat ataacttcgt
     4141 ataatgtatg ctatacgaag ttattaggtc cctcgaggta gttcagaatc cggagcagta
     4201 agtggagaag agctctccaa tagtgctcag tcttagaact attagctggg gatgtagtgg
     4261 atgggacaaa agacagcagt agccatttgt attgactctg acttcacatg agtatttctt
     4321 cccagctcct ggcctcacat gggtggtcac agggagaaac aagtactgta gcagatcttt
     4381 gagtttcaat gcaccttggt tggtgttggc aacaaacagg agcttctgca catcatgatg
     4441 actttacctc cattccctct acccagaacc ctaaattatt ctgttattgg atctgtgcct
     4501 gtaatatcag gatactacca tccaaatcta tctagatatc ttgtgcaaat attttattga
     4561 ttacaagggc tgtatacggg gttacaaaaa gacatgagct ggattggaca ctcctaaaac
     4621 caaaaacaac agtgacaata gagtctcagg atagcagagt ctgaaaatac cttggtctcc
     4681 cccattatgt gagcccttat ttgagagctt catgaagaca cttgtaacat attcaacgtc
     4741 ctgtactcca gttttgacta cgagatcaga ttttggaggt gtaagtaatc ttggctgagg
     4801 taacagtaat ctgcccattt agttcagtaa acagactgta gagtgtctga ctatactggg
     4861 ggaagaggag ccaatgacag ggtagggaga tgacagtggt gcatgatggg agaatcatga
     4921 aagtctggga agcatatgga ccagtgggaa ggataggatt agcactgcac ctctacatcc
     4981 atcaagagag acagagcgca caatggagca ggaaggataa aaaataaaga aagacagaga
     5041 caaacaaatt tgctgggaga gggaaagaca ttcagaccca cagaaaggag gcagcacaca
     5101 gagaaagatg cacagatcaa cccacatctg catgggttgt gagactgagc atagtgagag
     5161 cagtggtgga gcaatagaag agcggccgcc accgcggtgg agctccaatt cgccctatag
     5221 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5281 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5341 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     5401 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5461 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     5521 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5581 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     5641 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     5701 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     5761 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5821 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     5881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7141 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7681 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7741 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7801 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7861 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7921 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7981 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
     8041 agggaacaaa agctggtacc
//
