LOCUS       sequence-37637-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Diestra E, Fontana J, Guichard P, Marco S, Risco C
  TITLE     Visualization of proteins in intact cells with a clonable tag for 
            electron microscopy.
  JOURNAL   J Struct Biol. 2009 Mar;165(3):157-68. Epub 2008 Dec 10.
  PUBMED    19114107
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol."; date: "2009-03"; volume: "165(3)"; pages: "157-68. Epub 2008
            Dec 10"
COMMENT     Sequence Label: pMAL-c2x-MT3
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..19
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(621..638)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(621..637)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(630..652)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(816..833)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cctcgggatc gagggaagga tttcaagata taccatggac cccaactgct cctgctccac
       61 cggcggctcc tgcacttgca ccagctcctg cgcctgcaag aactgcaagt gcacctcctg
      121 caagaagagc tgctgctcct gctgtcccgt gggctgctcc aaatgtgccc agggctgtgt
      181 ctgcaaaggc gccgcggaca agtgcacgtg ctgtgacatg gaccccaact gctcctgctc
      241 caccggcggc tcctgcactt gcaccagctc ctgcgcctgc aagaactgca agtgcacctc
      301 ctgcaagaag agctgctgct cctgctgtcc cgtgggctgc tccaaatgtg cccagggctg
      361 tgtctgcaaa ggcgccgcgg acaagtgcac gtgctgtgac atggacccca actgctcctg
      421 ctccaccggc ggctcctgca cttgcaccag ctcctgcgcc tgcaagaact gcaagtgcac
      481 ctcctgcaag aagagctgct gctcctgctg tcccgtgggc tgctccaaat gtgcccaggg
      541 ctgtgtctgc aaaggcgccg cggacaagtg cacgtgctgt gcctaggatc ctctagagtc
      601 gacctgcagg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      661 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      721 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcag
      781 cttggctgtt ttggcggatg agataagatt ttcagcctga tacagattaa atcagaacgc
      841 agaagcggtc tgata
//
