LOCUS       sequence-376427-        7158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpa-11 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY93
FEATURES             Location/Qualifiers
     source          1..7158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2833..2853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2878..2898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2920..2940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4749..4771)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4871..4890
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4968..4986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5054..5158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5159..5227,5228..6019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5377..5396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6190..6778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6679..6698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7066..7087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7102..7107,7108..7125,7126..7132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7140..7156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7145..7158,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg acgaagaagc
      181 cgaagaatct taaaataatt tttattcaat ttttaaactt ttcaatttga tcccgccgcc
      241 ttatagattc acttttattt cccaaatctt gttgttgttt ttttttaatt taaaatgctt
      301 cattttccag taaattcgtg caaaattcaa gaatcttgac aaaatgacgt catcatcact
      361 caacacgcca cttttcacac taatttcttt gacttcggga aacacctgga gagcacaaaa
      421 cttgctctct ctttttctct cgatctatta atattcgagg gcagctagct gagatttttc
      481 caatttttta tatttgatac gaactttttt attcgaaaat tcgctaaaat tgccattgtg
      541 ggtctcgcca cgagcgaaaa ttttcaggta cagtactctt aaaaagaaat ctctggggta
      601 cggtagctga gaagtttctt cagaaatccc ccaaaattac aattttgggt cccgccacga
      661 gtgaaacttt tcaactacag taccccagag agtacccccc cccctctgta gctgaaaagt
      721 tttctcagtt ttttttcaaa gatttttaaa actaattttt gtgaaacatt ttcaacaaaa
      781 aacatttaag aatccgaaaa aagagtttta aaaatttaaa agtacatctc agagggtact
      841 gtacctggaa agttttgttc gtgccaggac ccaaagccag tttttttgcg ctctactgcg
      901 aaaatttcaa aattattgtg aaatttgttt cgaaatcgga aagttttcaa aacaaaaaac
      961 tggattttca aaaaattcgt cgaaaaatgg gccctggtac gagcgaaact tttcagttac
     1021 agtacccctg agattttatt ttttttttcg aattttttct actttttcac tgattaatca
     1081 caacaataaa taaatttgaa tcacaaaaaa aaagggaaaa tactagaata attgagcaaa
     1141 atgggtacgg tagctgaaca atttcgctcg tggcaggacc caaaaagacc gaattttctc
     1201 tttttatggc gaaattcttc tctccccctc tctaatacta aagatacagc acacacacgt
     1261 acttaattgc ggttatttgg tgctctggaa aattgttagg tggctcatat aattatagcc
     1321 aacccccttc cccacccgct cttcgtctct caaaaccctc tgttttagat gaattttaag
     1381 ctatgagtta tgccgttttt tgttgcgttt tcttgtaatt ttgggtctca cagcgaaaaa
     1441 aaagtgctct attgagaaaa ttgcgaaatt tactaaaaaa aaaagtagaa cttttccaca
     1501 agaaagcatt ttttcgcaaa aagttttaaa aagttctcga ggccacttgc tgtgtgacct
     1561 tgaaaagaga aaatgcggcc agtctcaatt aaaaattcca aaaaactatt tttttgggtc
     1621 ctggcacgac caaaactttt ttagctacag tacccccaaa atgtatttat ttttttgcat
     1681 ttccacgagt ttatttaatt tttttttcca cttttttact ggtaaaattt gaatatttga
     1741 atatttttcg aactgagaac tactgtacct gaaaattttc gctcgtgtga ggagccaaga
     1801 tggtaattat aactgaaata ttgcactatt tcgcaagtag gtaatattat attattatat
     1861 attggatgaa aagaagtcag aaaaaaaagt tgtcctcttc tcacatgctt ctaattttca
     1921 tctgcttttt ttgccttttt tcattttttt cacagatttt caaatttttc tccccttttt
     1981 ctcaaaactt ccagttttct ttgtgaattt tcgaatgact tttttgaata aaaaattgga
     2041 aaaaaaaaat caattttcgc cgaaaaaatc gaaacttgtt tcattgagct ctgacacact
     2101 ctaatgagct cagctcaggt gctcgaaaga gtatatttgc gaaaaaaaac taaatgtctc
     2161 tcacaagatt ttttttcgaa ttttacagaa atatgtttcg gtggctgcgt aaaaatggtg
     2221 gccgaggttt ctttttttcg agtggcctaa tttttatttt tatgttttaa ttctcggcca
     2281 cgaaatttgg gaaaactcag aaattcaagt tctgagaatt ctaaaaaaat taaaaaaccc
     2341 ccaaaatttc gatctcaaat tgtttttttc agagcatttt ttaccgcccc gtaaaactgc
     2401 tctaaaaaat ttttttccgc aagttttttt gctaattcta aacaaaggtt gtagtaggaa
     2461 aaataccctc gaaatttagg ctcttttttg gaaaactccg aaattgataa aattgctaaa
     2521 aaaattgctt ttggttttac cgaaattttt tgaattttca ctgaaatttg cgttttcttt
     2581 ttggttatac tttaatatcc aaaaatgttt tagtgagttt ttttttcaag ttaaaaaaaa
     2641 atttgtaacc tcatacatat atccccctga aacttctcca ttttcagcca aaaacccggg
     2701 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     2761 aggaggaccc ttggagggta ccggttttcc cagtcacgac gcgtggtacc gagctcagaa
     2821 aaaatgaccg ctccaaagaa gaaacgcaaa gtaccgagct cagaaaaaat gacggcacct
     2881 aaaaagaaac gcaaagtacc ggtcgccgag aagacagccc caaaaaagaa gcgtaaagtt
     2941 ccacgcgtca tgggagtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
     3001 tttcagcgcg ctgtctccaa gggagaggag ctcatcaagg agaacatgca caccaagctc
     3061 tacatggagg gaaccgtcaa caaccaccac ttcaagtgca cccacgaagg ggagggcaag
     3121 ccctacgagg gcacccagac caacaggatt aaggtggtgg agggaggccc cctgccgttc
     3181 gcattcgaca tcctggccac ctgctttatg tacgggagca agaccttcat caaccacacc
     3241 caaggaatcc cagacttctt caagcaatcc ttcgtaagtt taaacatgat tttactaact
     3301 aactaatctg atttaaattt tcagcctgag ggcttcacat gggagagagt caccacatac
     3361 gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc
     3421 tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     3481 acactcggct gggaggccag taccgagacc ctgtaccccg ctgacggcgg cctggaaggc
     3541 agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc
     3601 acatacagat ccaagaaacc cgctaagaac ctcaagatgc caggagtaag tttaaacata
     3661 tatatactaa ctaaccctga ttatttaaat tttcaggtct actttgtgga ccgcagactg
     3721 gaaagaatca aggaggccga caatgagacc tacgtcgagc agcacgaggt ggctgtggcc
     3781 agatactgcg acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac
     3841 aagtaagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc
     3901 tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca
     3961 gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc
     4021 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     4081 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     4141 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     4201 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     4261 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     4321 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     4381 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
     4441 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
     4501 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     4561 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     4621 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     4681 taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac
     4741 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4801 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4861 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     4921 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
     4981 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5521 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5581 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5701 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgatg aagcggaaga
     6961 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7141 tgtgagcgga taacaatt
//
