LOCUS       sequence-376428-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpa-3 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY96
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2256..2276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2301..2321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2343..2363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4172..4194)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4294..4313
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4391..4409)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4477..4581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4582..4650,4651..5442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4800..4819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5613..6201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6102..6121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6525..6530,6531..6548,6549..6555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6563..6579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6568..6581,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga tacactcaca
      181 cacacgcacc ggtgagctct cacttcactg atttgcacct gttattttcc gatgacaacg
      241 acaccatgcg cccatgattt tcagaattat gtgtagctga agggagccat ttttaaccaa
      301 tttcgttgtt atttcatttt ttgtgatttg atttttacga tattcttatg gcggttgaga
      361 aaaagaaaat ttaaatttgg tggcaagtaa atgggttttt agatttattc atttgaactc
      421 aaatagataa aattaatgaa aatgctgaca aatacaaatt cataatcaat gtgtgttagt
      481 tttgaaattt gtgaaaaaca tagttgtcaa ggatatttgt aaactttcta acaaactttt
      541 ccgtcgagtt gtataatccc tggcagatga tttttttttt gtaaaacccc atcaaaatta
      601 cttgcaaact tagaaatttg gggaaataca tgtgatttcc cccgaagaca tttgatttgg
      661 gacacattgc gaaaactcac gtaaatacta actttttcgt tgtttttttt aagaaacaaa
      721 acagttttta gaacttcctt acaagtattt attctagttg tctaaaaaca tttttttgaa
      781 aaacaaaact ttttttgtta ttttgaaaag tttcaatatc taattaccca gtggagacta
      841 gaaaattcat cattaaaata agtcatgttc atctttttta aagtaatatt taagtatttt
      901 tcaaattttg aattttatca ataaatatcg atcacaaacc tcatttttgc atttctaaaa
      961 gttagcgttt ctaaaagtaa atgccgtggg atattaagaa tcttaacact ctcgccaact
     1021 acagctctat ccttccttga ctactaaact agaactttcc gtcacttgac gcctcctgaa
     1081 attttatttt gcaaatagat gtttgcactt ttcgtactga ccactctgac ctcaatgatc
     1141 gtagacgagg tgatttctga tattatatgt tcatattgag aaacttggtt caatgttcaa
     1201 ttacaatttt tagaatctaa ctaattagac aaatttgaaa tttcccaatc gcacagattt
     1261 tggtgggttg tatcaaccgt cgtaattttc actacaaaaa tcaattttaa tactcgtatt
     1321 gattgtggac cctcaaatac tcctggaaac attatgcatt cattttgagt ctatttttgt
     1381 atgcttcttt tttatgtcta agcattcgtc gacatttgaa atatgcgtga aataattttc
     1441 cagtggaact agagctgggg ggcttcctat tgatttttga ttgatttttg aaaaagactt
     1501 tcgaccacga atatcaataa aataatctaa gtttccggga agatctgaaa catcaaaaag
     1561 aaacgactgg aactattcca tcgaacaata gtctatcttt cgttcattta gatgtgaaat
     1621 tcatgatgat gtacaaattg attctattcg aaagattaat gtattattga acattttcag
     1681 ctgaatcgaa catcttttat cactcattat tatgatattt gttcattttc aatgcgtcac
     1741 agcttggtgt cgttttgttg aattggcagc accatttctt gtgaaaaaat gcttgctgtc
     1801 gtctttcatt attgtcgagg tgaaacagaa aaagaaagag acacacgaga tatcaacaac
     1861 acaaaattct gaatatctga ctttggctct gaccgccgtc gccgccgccc gcccgctaac
     1921 gagcattttc tcattttctt ctcatttacc ccgtgtctac ttcttttctt atttttcgtt
     1981 tcgttaaact tcggttgtcg cataatcagg cctgcttttc atttttcact ttttgtttga
     2041 acttttgggc tttttagagt tgtgcttcat tcaaatctat attcaaatta cagaaaatta
     2101 ttagccaaag aacaattccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
     2161 ctaacataac atagaacatt ttcaggagga cccttggagg gtaccggttt tcccagtcac
     2221 gacgcgtggt accgagctca gaaaaaatga ccgctccaaa gaagaaacgc aaagtaccga
     2281 gctcagaaaa aatgacggca cctaaaaaga aacgcaaagt accggtcgcc gagaagacag
     2341 ccccaaaaaa gaagcgtaaa gttccacgcg tcatgggagt aagtttaaac agttcggtac
     2401 taactaacca tacatattta aattttcagc gcgctgtctc caagggagag gagctcatca
     2461 aggagaacat gcacaccaag ctctacatgg agggaaccgt caacaaccac cacttcaagt
     2521 gcacccacga aggggagggc aagccctacg agggcaccca gaccaacagg attaaggtgg
     2581 tggagggagg ccccctgccg ttcgcattcg acatcctggc cacctgcttt atgtacggga
     2641 gcaagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa tccttcgtaa
     2701 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagcct gagggcttca
     2761 catgggagag agtcaccaca tacgaagacg ggggcgtgct taccgttacc caggacacca
     2821 gcctccagga cggctgcttg atctacaacg tcaagctcag aggggtgaac ttcccatcca
     2881 acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag accctgtacc
     2941 ccgctgacgg cggcctggaa ggcagatgcg acatggccct gaagctcgtg ggcgggggcc
     3001 acctgcactg caacctgaag accacataca gatccaagaa acccgctaag aacctcaaga
     3061 tgccaggagt aagtttaaac atatatatac taactaaccc tgattattta aattttcagg
     3121 tctactttgt ggaccgcaga ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg
     3181 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
     3241 ttaatggcat ggacgagctg tacaagtaag cgccggtcgc taccattacc aacttgtctg
     3301 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     3361 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     3421 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     3481 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     3541 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     3601 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     3661 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     3721 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     3781 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     3841 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     3901 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     3961 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     4021 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     4081 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     4141 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4261 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4381 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4441 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6361 tcagtgagcg atgaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
