LOCUS       sequence-376429-        6579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  glc-3 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY89
FEATURES             Location/Qualifiers
     source          1..6579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2254..2274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2299..2319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2341..2361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4170..4192)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4292..4311
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4389..4407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4475..4579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4580..4648,4649..5440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4798..4817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5611..6199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6100..6119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6487..6508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6523..6528,6529..6546,6547..6553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6561..6577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6566..6579,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt gatcgtgaaa
      181 gccctcccgt ttatttttcc cctgatccta ttcatccttg tgttcgtctc taaagcttcc
      241 ttgttttttc caaccacaat acgtttttcc cttttttttc gaccttaatt cttgtcaaag
      301 tcgttttttt ttcgttttta ctccgtttcg ttttctcctt aactcaattc atgacaaaag
      361 aaaagctcaa agtcatacac ttttcaatgt taaaggatgc gtttcaaaat ttgatcaagt
      421 ggcatttcac acgaaaacga gaaaaagtca gttcgaaagc caaatctcaa gaaaaatcag
      481 gtttcaatga tttcgtgaaa ctcagattgc cttatttcaa tgatttttga gatgtgcttt
      541 gaaaacatga cgaacaaaaa actagcaatt ttatttttga agtttttcat gtaaggagcc
      601 tgtagatttt ctaccaaaaa taacccaaca gttttagtat gccaaatata actttgaaaa
      661 tttcagagag ttaaaaaaaa ctcattataa tttttgaaaa aagttctagg agcatctgta
      721 tcagttttca attcccgcgc aattttttcg gctaaatttg gcgttttttc tctgaatata
      781 gtatttttga aacatctttg gtcaaatttt tagacctttt taccgccaaa aaagtttata
      841 caaaagtaaa tttgtataat tttcaagata cttccataaa tttaggcgga gagttttcca
      901 aaatttttga aagcgtctta actagatcat taatttttgc ggaaattcaa aaataactca
      961 gactgattcc gttctgattt gatttttcaa atatggaaaa atgcttaaaa atttttttga
     1021 aaaattaaat aattctttac agttttttgg taaagttgta aattttcgca gtgaattttt
     1081 gactttctga tagattttga aacaagtttt ttttttccga atttcaaatt tatttccccg
     1141 gaaaattttt tcaactgaaa ttttggaaaa gaaaccgggc tcttttgtcg gcgtcgtcag
     1201 atgaatcccg ccccattctt aggttcaaaa agcactttga aattacgctt acaactgaaa
     1261 actgaaattt cccacaaacc gtgctgcaac agactccaat atgaatgaaa tgctggtttt
     1321 ttatagatca agattcgatt tttagttcag tattcgaaaa tttaatcaaa ggctgttgga
     1381 actgaaaaat tacttttacg gctaaaaaaa tacactttta aaaagcttct gttgaacaag
     1441 catagttcgg acgaaaattg ctagcataaa caattttaaa gtacattata tttggttgaa
     1501 ttttcactga aatttgaagt aaaaaatcga ctcacccatg tatcccagca gtttttcaac
     1561 tcatatataa cagactgccc gtgtccccac actccttcac tcatttaaaa cgagagcaat
     1621 ttggtgacgg gaaaaccaaa aaaaaaactt aaagaaacct cttctctctt tcgttctcac
     1681 gcatcattgt cttccttttt ccctcttttt accaccactt ttctcatttt cggaaagttt
     1741 tttcttcttg tttcatgatc tcttcttttt taaaagtctc aaagtgttgt aacacagatt
     1801 tcttctaaat aagcttaaaa tctttcagtg tctaattgat agaagaaaaa agaaggaaaa
     1861 cggagacgag cgtatggaat ctgtaaatcc ttcgtctatc catccgtctt cttcatttct
     1921 cacacaagcg gctcgattca acgactccta atggctcatt agccttgtta tgactttctt
     1981 tcgtactact cgacacactg tgcgcatcaa attaatactt cttgccgctt ctgcttctga
     2041 atatattctg ccgccgccgt ctctggaggt cacatcatca ttctctcgga ttttcgcgtt
     2101 ttcggttaat acttgcccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     2161 aacataacat agaacatttt caggaggacc cttggagggt accggttttc ccagtcacga
     2221 cgcgtggtac cgagctcaga aaaaatgacc gctccaaaga agaaacgcaa agtaccgagc
     2281 tcagaaaaaa tgacggcacc taaaaagaaa cgcaaagtac cggtcgccga gaagacagcc
     2341 ccaaaaaaga agcgtaaagt tccacgcgtc atgggagtaa gtttaaacag ttcggtacta
     2401 actaaccata catatttaaa ttttcagcgc gctgtctcca agggagagga gctcatcaag
     2461 gagaacatgc acaccaagct ctacatggag ggaaccgtca acaaccacca cttcaagtgc
     2521 acccacgaag gggagggcaa gccctacgag ggcacccaga ccaacaggat taaggtggtg
     2581 gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc
     2641 aagaccttca tcaaccacac ccaaggaatc ccagacttct tcaagcaatc cttcgtaagt
     2701 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagcctga gggcttcaca
     2761 tgggagagag tcaccacata cgaagacggg ggcgtgctta ccgttaccca ggacaccagc
     2821 ctccaggacg gctgcttgat ctacaacgtc aagctcagag gggtgaactt cccatccaac
     2881 ggccctgtga tgcagaagaa aacactcggc tgggaggcca gtaccgagac cctgtacccc
     2941 gctgacggcg gcctggaagg cagatgcgac atggccctga agctcgtggg cgggggccac
     3001 ctgcactgca acctgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     3061 ccaggagtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggtc
     3121 tactttgtgg accgcagact ggaaagaatc aaggaggccg acaatgagac ctacgtcgag
     3181 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt
     3241 aatggcatgg acgagctgta caagtaagcg ccggtcgcta ccattaccaa cttgtctggt
     3301 gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa
     3361 cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta
     3421 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     3481 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     3541 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     3601 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     3661 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     3721 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg
     3781 ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt
     3841 tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc
     3901 ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg
     3961 agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta
     4021 gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat
     4081 acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt
     4141 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4201 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4261 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     4321 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
     4381 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4441 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4561 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4621 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4681 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4741 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4801 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4861 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4921 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4981 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5041 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5101 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5161 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5221 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5281 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5341 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5401 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6361 agtgagcgat gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6421 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6481 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6541 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
