LOCUS       sequence-376430-        7723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpc-1 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY98
FEATURES             Location/Qualifiers
     source          1..7723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3088..3108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3130..3150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5314..5336)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5436..5455
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5533..5551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5619..5723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5724..5792,5793..6584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5942..5961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6755..7343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7244..7263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7497..7514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7631..7652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7667..7672,7673..7690,7691..7697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7705..7721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7710..7723,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ctcgaatctt
      181 gtgacggata taaaacaaag tatactacaa aaactagatg ttactagtac attcttctaa
      241 ttcacaactt cattttcaag tggaaatttg ctaaacacct gtttcctagt caaattgggt
      301 taatgttttg tgcttttctg agtattttct catatggaaa atgttgtttc tatacaacat
      361 caagttagat acatattgta gctagcattt cgattcttga aaagttcaac ttcactttgt
      421 atgtgcgcaa acgtgactct tttttacttt tattgtaaag tgcgttttca ttttccgtta
      481 aattgaacac cctgttaaat tactcttgtt tacctttttg taggcattct tgctacctac
      541 ctgctaattc tctgcacaaa cgcgtcgaag ttcagaacta taacgtcatc tcagagaagt
      601 tgaagaaaaa taacaatcaa gtaataacta cgtaatcact attagtatct ctttatttta
      661 tatagtttcc gcagatgacg aagttgtgaa acaaataaac aatacatgtt ttgtttgaaa
      721 atgggtaaat ggggtaggtg tgtgtagttt ttttttaagg atgtcatgtg ggcgggacta
      781 ttatgtcatc atattagttc tttcttgaga tttcaaattt atgactctta tgcaatcggc
      841 atttaagttt actttgattt aaaaaaatta aaacaaatgg gcttacgaag tccccgaatg
      901 ttagtctcat ccaacttggc gctatattaa attatgataa gaagctgcaa aaacatgttc
      961 aaatgtattt tattgacaac aaaaggtggc aattacttga ttattgcctt ttttgagaat
     1021 actatagcac tggagtatgt ccgaaatcgt acagtgtttg aacactaata tttgaactta
     1081 tagttaagtt aaccaaaaaa tttttaagaa aaatgttttt gatcaatttc caaaaaatgg
     1141 tagttttaat tagtctcatt tttagaaaca ataaaaaaat ttttaaaaat ttgttaaaaa
     1201 ttttactata atatttagcc attttggcac catgagagtg gtttttaaat ttttaccttg
     1261 ttctgctgct tcacctttaa tgacagttcg atatataatt atactactta attttcatta
     1321 gtttaatttt aatattgcca gtttgtttga ctatatattt ctattagtta ccacaaaacc
     1381 taatcacctt tctttgcagc tttgtcaaaa tatgattaga gtataaaacg catagctcat
     1441 ttagtaacta tgggcaacca cacctaataa aagtttggat aagaaagctg attggtacga
     1501 gttatgctat ctggttatga aatgatgaaa agaaaacata aaacaggtga aagtaaagag
     1561 cgagctgctg acttgttgag ttttctggac tctttttttt ttgtaaagat tttgttaaaa
     1621 tcacgaaaac tcgacagtaa ctctagtata gctcgtttct ttggatttta cttctgactc
     1681 acgaagcgtt aaattttgtt ttgaatagtg aagagattta tgtggtttat gcttaacgtt
     1741 ttaattatgg gagttatcag caatgaaagc tttttacata tatacaataa gatccattta
     1801 aagcgtataa aaagtttagt tctgtccaaa taaaaacgaa aattggggtt tcacattatt
     1861 tgagaaccta gttgtataat tttcagtaat tttcatattc ttctcagttt gttataattt
     1921 tctgctgtcc aacgcaattt tcgttttatt aaaatagcat ataaaataat ttaattttga
     1981 ttaaaattca agaaatacta aatgtgatcg taagctatta actaaataca atttcgtgtt
     2041 gaatattttc aaagtcagaa agttaataca aatcatcttg cctgttcctg attttttaca
     2101 aataacaaat ctaggaatcg agacaaaaaa ccaaatctat attcaaatgg tcactgacaa
     2161 atttttgaat acaaatccaa tcaaaacaac tataaagagt tggattcgat tcattcagtt
     2221 cttgttttga tcaacagagc tttgcaaact gagtgatttt tagattgaga aagaaatgag
     2281 ttagtagaag tattagtttt taagaatttt agccgcaacg tattttgtat ggttattttg
     2341 atgaattatt aaaaattaac atttttctgg ccaatttgat atttacgtat aaattatatt
     2401 gaatgtagca tatcaaaaat attcaaaaat gttcaataat aaaatcaatg ttaaatccgc
     2461 tggcgccaaa atccctccga atcccctgaa ttgtggattg actaagagta aaaggaagag
     2521 aaacgaaaaa gggaggacga cttaatcccg aacaatattt tttatgatga agagcgcata
     2581 gggccgtcgt cagttctcac actggatttc actttttacc gcctctcttt tgtcgtgaag
     2641 agtctcagaa tccagatgta aaatcctctc caatctattt attaaacata gctcagtctt
     2701 caatctttta attataaaca ccgttttagc acatgagctc ttctgcctcg aaacccttta
     2761 atttcgacaa aagagcaaat gctccgatcg taaaattggt agtttcaccc accaaaccta
     2821 ctcattttgc gtcgacacca aacttgtctc ctcgcttttt ttcttcccag ctaccttact
     2881 catttcacca gttttcgcta ttgagaagaa gatttgtttt atcatatttt gaaaatttat
     2941 tagcgtttct ttcagttcca gaaaccatct gagcccggga ttggccaaag gacccaaagg
     3001 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tgcttggagg
     3061 gtaccgagct cagaaaaaat gaccgctcca aagaagaagc gcaaggttcc agtcgagaag
     3121 atgactgccc caaaaaagaa acgtaaggtc ccgcggatgt cagagcttat taaggagaat
     3181 atgcatatga aactgtacat ggaaggaacc gtggacaacc atcactttaa gtgcacttcg
     3241 gagggggaag gcaaaccgta cgagggcacc cagaccatgc gaatcaaggt ggtcgaggga
     3301 ggcccactgc ccttcgcctt cgacattctg gtaagtttaa acatatatat actaactaac
     3361 cctgattatt taaattttca ggctactagc ttcctgtacg gcagcaagac ttttattaat
     3421 cacacccagg gcatcccgga cttcttcaaa cagtcgttcc ctgagggctt cacatgggag
     3481 cgtgtgacca catacgaaga cggtggcgtt ctcaccgcta cccaggacac cagcctccag
     3541 gacggttgcc tcatatataa tgtggtaagt ttaaacagtt cggtactaac taaccataca
     3601 tatttaaatt ttcagaagat ccggggagta aacttcacat ccaatggtcc ggttatgcag
     3661 aagaaaacac tcggctggga ggccttcacc gagacgctgt atccggctga cggaggcctg
     3721 gaaggtcgaa acgacatggc cctgaagctc gtcggcggta gccatctcat cgcaaacgct
     3781 aagaccacat atagatccaa gaaaccggta agtttaaaca tgattttact aactaactaa
     3841 tctgatttaa attttcaggc taagaacctt aaaatgccgg gagtctacta tgttgactac
     3901 agactggaac ggatcaagga ggccaacaac gagacctacg tcgagcagca cgaggttgca
     3961 gttgccagat actgcgacct cccgagcaaa ctgggtcaca agcttaatcc taggataccg
     4021 gtgggtgaag acccaccaaa gccatctgcc aagggagcca agaaggccgc caagaccgtt
     4081 acgaagccaa aggacggaaa gaagagacgt catgcccgta aggaatcata ctccgtctac
     4141 atctaccgtg tcctcaagca agttcatcca gacactggag tttcctccaa agccatgtct
     4201 atcatgaact cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc atcccgtctt
     4261 gctcactaca acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc tgtccgtctg
     4321 atccttccag gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc cgttaccaag
     4381 tacacttcca gcaagtagga attccaactg agcgccggtc gctaccatta ccaacttgtc
     4441 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     4501 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     4561 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     4621 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     4681 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     4741 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     4801 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     4861 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     4921 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     4981 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     5041 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     5101 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     5161 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     5221 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     5281 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5341 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5401 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     5461 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     5521 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5581 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5641 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5701 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5761 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5821 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5881 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5941 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6001 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6061 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6121 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6181 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6241 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6301 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6361 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6421 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6481 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6541 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6601 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6661 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6721 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6781 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6841 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6901 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6961 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7021 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7081 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7141 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7201 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7261 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7321 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7381 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7441 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7501 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7561 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7621 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7681 ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
