LOCUS       sequence-376431-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-26 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY19
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5609..5629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5651..5671
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7835..7857)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7957..7976
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8054..8072)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8140..8244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8245..8313,8314..9105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8463..8482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9276..9864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9765..9784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10018..10035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10152..10173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10188..10193,10194..10211,10212..10218)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10226..10242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10231..10244,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aatgatctct
      181 caggttgtac attttctatc ctcttttttt tactattgtt tctcaactaa tttacagcaa
      241 ttgtaaaagt aataaaaagt ttatgtgacc ttttgtgcgt cgcgttttga aacttattag
      301 caattttaac atagtgagtt gttttaagat accctgtgtt ttggaatcaa acattgtgtt
      361 ttctgaaaaa caaactttga aatggatact ctaatttgca acaacaaatt tgtaatttta
      421 gctttggcga cctattttag ttttgttcca aaacattact attcgaaaag cccacgcaaa
      481 taagttgtgt tttcgaccac taaacgactg aagaatcaca tatccacttg taagattttt
      541 aaaccctgat aaacttctta gctttaaatc agcggtacct taattaactt tttaaaaagc
      601 ttgaaacact tccttgacta aaatcagctg ttgacctcta ctgcaacgtg tttagattca
      661 atgccaccat ttttcacatt tataagactt tttgtagtgt ttttgtgtta aataatcact
      721 ttccataact ttcatcaagt aattagaagg aagcaaagat ttttcatttt aattgtctgt
      781 ccattaaaat cttgtttcat aagattttgc aatttatgca aaaaaacttt caaaatttga
      841 acatgtttct aaaacagcaa aacatcagta tacgttcaaa ttagaaaatc ttgcaaaaag
      901 tgaaacaatt gtaaagttta ccacactttt ttttggcaat ttagccattt ggaagtgtat
      961 tcaaattttt ttaagtcgga gttttcgaga aaataccaaa ttgaaccatt tttgacaatt
     1021 acaattctac aattatcaaa tagaaaaact ttgtcaaaaa acataaattt ttctacaaac
     1081 tcataaaaat ataaggattt ttgattatcg gtcccgggta ttgttttttg aagataattt
     1141 gatgaatcgg cactttcggc aaatgccaac tctcaaaaat gcaattattg ctcaatgttg
     1201 gtataaaaca aaatattcaa atttaatgaa aatactgaaa cttaaaattt taaattgcat
     1261 ttttaaactt gaaataatgt tttaaaatat gtttttaagt ttcgaaaaac tctcatgaaa
     1321 ttttcttttc atactgtgta acgaccacta catattttta aattatgatc ccgaccagac
     1381 ctgtgtggca ttcggaattt ttcggcataa cggcataacg gcatatgccg ccaaaaattt
     1441 tttcggcata tcggcatatg ccgttatgct gtccattttt catttcggca taacggcatt
     1501 ttcggcatat gccgtgaatt ttaaaatcgg catttcggca tatgccgcgc tccaaaaacc
     1561 ggcattatgc cggcattcgg cattcggcat ttcggcattt tgaactattg tccagttaca
     1621 aaaaaatcaa caatatttga atgttttcaa tcaaacttcc atgtgctttt tatcattacc
     1681 aatatctaaa aaatagtatt cgtctgattt tggtatgtga cgtagcacta caggtatgca
     1741 caattttttg cttttgacaa gatattgagt tggggttact ggttagatgt ttttatgttt
     1801 ttgtaggagt actttaatgc gaccaacagc tatgaagccc cctccacttg agctgtatag
     1861 ggcatctgca aacttttggg aaactaatat tgacatcgga tgcagcatac caaaaaacct
     1921 ttgtatctac aattttatac tcacatttca aaattgaagg caaaagagca aaattttgaa
     1981 aagtcgaccg tccaaatgct atgaatccgt cagaaggtgc tcatgcgact tacatattag
     2041 atatatttca ttcgatctta tatgtaagtc caatgagcaa cttctaacgg atcattagtg
     2101 agattttcga gaatttcaca caaaattctc attttcttcg caaaacatta aattttccat
     2161 tttcaaaata atatttaatg gagtactctg gtagtgtact gtttgttttt tatatcatgg
     2221 aaattactga aaacgtgcaa aaaaaattgt aagtatgtta aagggggagt acagtttgtg
     2281 ggtatttttc ttaaatagac taaaactggt ccaaaaccac agaatttcat aatgagactt
     2341 cacaaaaaat ttccaaaaat tttttatggc cggtcaaatt ttcgaaaaaa aaagagccaa
     2401 attttgctaa aatcaaaaaa tttcgcgcac ttttctttgt cacagctgct gggcaatttg
     2461 tgaaaatttt ttgcgaagtc tcattatgaa attcggtggt tttggaccag ttttagtcta
     2521 tttaaacaaa atacccacaa actctactcc ccctttaatg tacacgcctt gaaacatatt
     2581 ggtgcatttt cagacaattt tcgagtcaag ccaaaattgt tgcagaaatt ttccaaatgt
     2641 tcgagcaaaa agcttcaaga aatctcaaaa atggaacaat ctgcgtagaa accttactat
     2701 ttgcataatg ccgtcataat gtcggcatta tgccgttatg ccggacattt tatttttcgg
     2761 cataacgtca ttttcggcat ttgccgtgaa ttttcaaatc ggcataacgg catatgccgt
     2821 gctccaaaaa ccggcattat gccggcatcg acatatgccg aatgccgaat gccgcacagg
     2881 tctgatcccg agagttctct catttggaga atatcaaaat ttccatttca tttattgtaa
     2941 caacattaaa agaaatttga cctagcaatg gaggcggtaa gaaattgcag aaaaacgagg
     3001 ttgtactaag aaaccaggta attcaatatg cgtgatctca ttttacaatt aaagctcatc
     3061 aaagtggtgt gggaaatgcg caatgtgctt catccatttg ttacatttcg ttgctcattt
     3121 gatgtttttt ttctagcgat cgaaccgcgt aggtttttgg ggccacgttt tcaggaaaat
     3181 gacttcagaa taagaattaa gacgtaaagt gaatttatta ttttgtcttc atggcgtcgt
     3241 gtttgttttc cattgaatag caatgatttt agtattgctg acttcattat ttgatttact
     3301 ccttcatcag atctattttc ttagaagtat caaaacttta gttaatagta aaattatggt
     3361 agacaagatg tatttacact ctcttccaaa atattgtggg cctctgcaga tatattttgc
     3421 agtactcgta aaataataaa gtttgtatag atcgttatat tcaagtacct aataacttcc
     3481 cctaacttga tttgagaagt gcacttttat gggattcttg cgtgattttg gttccactat
     3541 tgttactgag tgagttgata ttcgaagaag ttccacataa acattccctc tataaaatta
     3601 acttataaaa gtcgttttct ttaaattttc tctccctgca ttttcttgca gttaggtaat
     3661 gttggtaatt gtgtagtata atctggcccg agcatattca ccattttgaa tttttagtgt
     3721 tcctttacaa attaagttta ggattggtgt ttttctaacc ctaaatatgc aaataaattt
     3781 cacgttttca ttaaaggttt gataacgtat tggaagtatt ggaagtattt ggaagtatta
     3841 ggtatacttc actaatactc taatgttata ttaatcttgt aaaggctgaa catgctagct
     3901 ctgaccttcc agtgcatgtc attgattgtg atagtgtact tcaatattca attttcaatc
     3961 acgtaatcaa atagacggct ataaccagat gataatacag accctatttc acttccaaat
     4021 agaatagact attgtgacca atctacattt ctcccagtat tggaagtatt tggaagtatt
     4081 aggtatactt cactaatact ctaatgttat attaatcttg taaaggctga acttgctagc
     4141 tctgaccttc cagtgcatgt cattgattgt gatagtgtac ttcaatattc gactaaaaat
     4201 gacatgagtc gataactcaa ttttcaatca cgtaatcaaa tagacggcta taaccagatg
     4261 ataatacaga ccctatttca cttccaaata gaatagacta ttgtgaccaa tctacattct
     4321 cccactattt tttgatgatt tcaataccta aattaatact tctaagcaac gagacacatc
     4381 atacttgtaa tgattcatca atcgtatgaa tcatagtcaa gcttcaagat tttttttgtt
     4441 ttaattaaga aaagttggcg gcctttgcga aaccatatat gagcattcaa tcaatttgtc
     4501 aggattcaag aagctcacta ttgtgagaaa ctgcgcagtt tggatttcac catttccatc
     4561 atgaacataa aattttatga cccgtacgtc atttttgagc cctggaacgc atcacctttt
     4621 gttcgccggg ttttatggcc caacgtgagc ttctgttatt tccgggtaac agaatgccga
     4681 aaaaggatcc gaaaggagga gcacggtggg gtttgtcatg atcggttggt atgctccacc
     4741 tactttttcc atttatattt cgtatagaaa ctgctaattt ccccgtcatc cccccccccc
     4801 cccccacttg ctcctccttc cacactttag tctcttcata cgatcatcat tgaaattctt
     4861 cacgagaaac aattgtagga atgaactcct cgtcatttct attttatatt ttccctcttt
     4921 gccctgaaaa caaaccaaat cagttaatag caatctcact ggtattagtt gttgtttttt
     4981 ttccagtgaa ctcctaattc ctaaaacaca gacttactca ctttttctca caattttctt
     5041 tctatcctaa aaaaacgaaa gtgtaaaatg cgacgacttc tttattttgt ttccgagctg
     5101 cgctgagctc tcggcagaca tcacattgcc tcctatgttt taattaattt ctctatgaaa
     5161 gtatgaagac aaaagagaag tatctaattg ggatatcttc agatccttga aactatactt
     5221 ttgcccattt ttatcctttt taagagcaag gaagtaatca tgagaaatat actagatcgc
     5281 ctatgtaaat aggaacgaaa tttgacatgt tgaaacttcc tcgttggttc ctactatgta
     5341 tattttggga agccacataa tagaaaaact aggaaccatt tcaagtttcc ctaatccccc
     5401 cgtttttatc ttccaaattt aaaacaaatt gagtctaact atattttaat atatttttga
     5461 aggtaacccg tcaagaacac ttacagtttt cacacccggg attggccaaa ggacccaaag
     5521 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttgcttggag
     5581 ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag cgcaaggttc cagtcgagaa
     5641 gatgactgcc ccaaaaaaga aacgtaaggt cccgcggatg tcagagctta ttaaggagaa
     5701 tatgcatatg aaactgtaca tggaaggaac cgtggacaac catcacttta agtgcacttc
     5761 ggagggggaa ggcaaaccgt acgagggcac ccagaccatg cgaatcaagg tggtcgaggg
     5821 aggcccactg cccttcgcct tcgacattct ggtaagttta aacatatata tactaactaa
     5881 ccctgattat ttaaattttc aggctactag cttcctgtac ggcagcaaga cttttattaa
     5941 tcacacccag ggcatcccgg acttcttcaa acagtcgttc cctgagggct tcacatggga
     6001 gcgtgtgacc acatacgaag acggtggcgt tctcaccgct acccaggaca ccagcctcca
     6061 ggacggttgc ctcatatata atgtggtaag tttaaacagt tcggtactaa ctaaccatac
     6121 atatttaaat tttcagaaga tccggggagt aaacttcaca tccaatggtc cggttatgca
     6181 gaagaaaaca ctcggctggg aggccttcac cgagacgctg tatccggctg acggaggcct
     6241 ggaaggtcga aacgacatgg ccctgaagct cgtcggcggt agccatctca tcgcaaacgc
     6301 taagaccaca tatagatcca agaaaccggt aagtttaaac atgattttac taactaacta
     6361 atctgattta aattttcagg ctaagaacct taaaatgccg ggagtctact atgttgacta
     6421 cagactggaa cggatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggttgc
     6481 agttgccaga tactgcgacc tcccgagcaa actgggtcac aagcttaatc ctaggatacc
     6541 ggtgggtgaa gacccaccaa agccatctgc caagggagcc aagaaggccg ccaagaccgt
     6601 tacgaagcca aaggacggaa agaagagacg tcatgcccgt aaggaatcat actccgtcta
     6661 catctaccgt gtcctcaagc aagttcatcc agacactgga gtttcctcca aagccatgtc
     6721 tatcatgaac tcttttgtca acgatgtctt cgagcgtatt gctgctgaag catcccgtct
     6781 tgctcactac aacaagcgtt ccacaatctc atcccgcgaa attcagaccg ctgtccgtct
     6841 gatccttcca ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa
     6901 gtacacttcc agcaagtagg aattccaact gagcgccggt cgctaccatt accaacttgt
     6961 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     7021 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     7081 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     7141 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     7201 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     7261 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     7321 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     7381 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     7441 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     7501 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     7561 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     7621 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     7681 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     7741 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     7801 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7921 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7981 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     8041 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     8101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10141 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10201 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
