LOCUS       sequence-376432-        6425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpa-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY30
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1784..1804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1826..1846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4016..4038)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4138..4157
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4235..4253)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4321..4425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4426..4494,4495..5286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4644..4663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5457..6045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5946..5965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6199..6216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6333..6354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6369..6374,6375..6392,6393..6399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6407..6423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6412..6425,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cgtttcctgg
      181 aacttgtgaa gaatttttct gcaacaacgt ggtaaataat aaaagctttc caaataagtt
      241 atcaggtcgt cgccaggaaa ttgctaggaa attgctaggt ttcaaggttg tcagaaagta
      301 gaaaaagttt gacaacgtat ttaaatttca aattatgcct aaagaataaa tatctagact
      361 ttatgaattt ttttttctat gtacctttaa accctcacaa atatatcccc ttgttctctg
      421 gttacgttga cttttttact atataatttt cgaaaaacct tgtcttgagt atcatcttac
      481 tttgtttgtc aatagtaatt tttctttcat ttttctttac aatatttcgt ttcccgaaca
      541 tttgaaatat aagccaaatt ttggttttca ttgagaaaac tctacatttc ttgaaaaaaa
      601 actacctgaa tttccagtaa tttctcatct tcatataatt ctaaaaacta aatttttaat
      661 gtcatgagag aattgaaaag tagttgggga aattaaattt cacctgcagt tgagagagta
      721 tagaaggact caacgcttac attctttatg acgtatcagc ttgaataagt atttttccag
      781 acttatatca gactaagttg aaaaacgggc agtactttta tttaatttat tcttgtgtac
      841 tttgtttctt tgagatcaag ttataagcac tattttataa ccaactttta aatattgtta
      901 tatttttctg ggaagtgaat tgattaaata agttttaaat ctttgagaag ttagcaatac
      961 aaatataatg tgagataatt gtgaacaaga atttaatatt gcttttgaat ctgtattaaa
     1021 caaacgatga aatattagaa gttaaatttg aacattgtgg tacaatacta ataaatgaaa
     1081 tacggttggt attaagtaaa atatgagtca gtatatccct ttaaaatcca tttttatttt
     1141 aaaatctgta tatttctgtg aagcctgccg ctgtttatat gttaaaaagg aaggttttac
     1201 tttctcaaat acttaatatc aggtttcata caatttttga aaaaaaaacg acaaatcttc
     1261 agttgtgctt ttttctttct ccatcttgtc atcatttcat cattttcaat gtattctata
     1321 attatttcca gctgccatta aatgcaaccc actttaatat ttttcttcct tttttccgtc
     1381 tgttccgaaa cccttgaaat cgactttttc attcactgaa ttcactttct ctgcctcgag
     1441 ttgtttatgc ggcgaaaaaa aatctgtaga ggtatacgaa aaagctaatt aggtggtttg
     1501 ccgtctagta atactcgatt atatatttct tgtcgtcatc gttaccactg ctctccattt
     1561 tctctcctca ctcacaaaaa cttgaatatt cttcgcttcc tgttgtgatc agtgaaaatt
     1621 cgtctgattt cttcaatatt attcaatgtc atttattcga agacttcagc ccgggattgg
     1681 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     1741 gacccttgct tggagggtac cgagctcaga aaaaatgacc gctccaaaga agaagcgcaa
     1801 ggttccagtc gagaagatga ctgccccaaa aaagaaacgt aaggtcccgc gggtaagttt
     1861 aaacagttcg gtactaacta accatacata tttaaatttt cagcgcgctg tgtcaaaagg
     1921 agaagagctt attaaggaga atatgcgttc caagctctac ctcgagggat ccgtcaacgg
     1981 acaccaattc aagtgcaccc acgagggaga gggaaagcca tacgagggaa agcaaaccaa
     2041 ccgtatcaag gtcgtcgagg gaggaccact cccattcgcc ttcgacatcc tcgccaccca
     2101 cttcatgtac ggatccaagg taagtttaaa catatatata ctaactaacc ctgattattt
     2161 aaattttcag gtcttcatca agtacccagc cgacctccca gactacttca agcaatcctt
     2221 cccagaggga ttcacctggg agcgtgtcat ggtcttcgag gacggaggag tcctcaccgc
     2281 cacccaagac acctccctcc aagacggaga gctcatctac aacgtcaagg tccgtggagt
     2341 caacttccca gccaacggac cagtcatgca aaagaagacc ctcggatggg agccatccac
     2401 cgagaccatg tacccagccg acggaggact cgagggacgt tgcgacaagg taagtttaaa
     2461 catgatttta ctaactaact aatctgattt aaattttcag gccctcaagc tcgtcggagg
     2521 aggacacctc cacgtcaact tcaagaccac ctacaagtcc aagaagccag tcaagatgcc
     2581 aggagtccac tacgtcgacc gtcgtctcga gcgtatcaag gaggccgaca acgagaccta
     2641 cgtcgagcaa tacgagcacg ccgtcgcccg ttactccaac ctcggaggag gaatggacga
     2701 gctctacaag cctaggatac cggtgggtga agacccacca aagccatctg ccaagggagc
     2761 caagaaggcc gccaagaccg ttacgaagcc aaaggacgga aagaagagac gtcatgcccg
     2821 taaggaatca tactccgtct acatctaccg tgtcctcaag caagttcatc cagacactgg
     2881 agtttcctcc aaagccatgt ctatcatgaa ctcttttgtc aacgatgtct tcgagcgtat
     2941 tgctgctgaa gcatcccgtc ttgctcacta caacaagcgt tccacaatct catcccgcga
     3001 aattcagacc gctgtccgtc tgatccttcc aggagagctt gccaagcacg ccgtgtctga
     3061 gggaaccaag gccgttacca agtacacttc cagcaagtag gaattccaac tgagcgccgg
     3121 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
     3181 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
     3241 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     3301 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     3361 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     3421 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     3481 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     3541 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3601 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3661 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3721 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3781 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3841 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3901 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     3961 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4021 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4081 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4141 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4201 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     4261 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4321 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4381 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4441 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4501 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4561 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4621 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4681 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4741 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4801 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4861 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4921 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4981 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5041 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5101 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5161 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5221 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5281 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5581 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6121 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6181 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6241 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6301 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6361 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6421 caatt
//
