LOCUS       sequence-376434-        5451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nlp-12 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY31
FEATURES             Location/Qualifiers
     source          1..5451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             810..830
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             852..872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3042..3064)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3164..3183
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3261..3279)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3347..3451
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3452..3520,3521..4312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3670..3689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4483..5071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4972..4991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5225..5242
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5359..5380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5395..5400,5401..5418,5419..5425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5433..5449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5438..5451,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cgtcaagctc
      181 agagcaacaa aggaggattc ttgggaagca cggtgagttt ttcgcccttt ttctggcaat
      241 aaaaacttat tttctatttt ccagatcaca ttcacaattc tcttcgtctt catcgccgtc
      301 ttcgcctact tcttgatcga gaacgccgag cagttgaagc tcgtggccga gacgaatccg
      361 gaggatacta tttaaattat tttttaaatt tctatttttt tctttgactg ttgaaatcgc
      421 tatttttacc cctctccctc ccccatcact ctttttcttt aaatcctttc accccatttt
      481 catcacttca tttggataaa cgctatgaat ctctctctca gtttttacga gcccatcaac
      541 tccagaagct aaccaaaatg gtagggggga aaggcgtggt ccccccggct ccgttgggaa
      601 atatgacgaa tctgtagttt actgatgggg gggggggtgg tataaaagcc cgacacctcg
      661 actcggcgaa acttatttca attgcctccg acaaacccgg gattggccaa aggacccaaa
      721 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      781 gggtaccgag ctcagaaaaa atgaccgctc caaagaagaa gcgcaaggtt ccagtcgaga
      841 agatgactgc cccaaaaaag aaacgtaagg tcccgcgggt aagtttaaac agttcggtac
      901 taactaacca tacatattta aattttcagc gcgctgtgtc aaaaggagaa gagcttatta
      961 aggagaatat gcgttccaag ctctacctcg agggatccgt caacggacac caattcaagt
     1021 gcacccacga gggagaggga aagccatacg agggaaagca aaccaaccgt atcaaggtcg
     1081 tcgagggagg accactccca ttcgccttcg acatcctcgc cacccacttc atgtacggat
     1141 ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtct
     1201 tcatcaagta cccagccgac ctcccagact acttcaagca atccttccca gagggattca
     1261 cctgggagcg tgtcatggtc ttcgaggacg gaggagtcct caccgccacc caagacacct
     1321 ccctccaaga cggagagctc atctacaacg tcaaggtccg tggagtcaac ttcccagcca
     1381 acggaccagt catgcaaaag aagaccctcg gatgggagcc atccaccgag accatgtacc
     1441 cagccgacgg aggactcgag ggacgttgcg acaaggtaag tttaaacatg attttactaa
     1501 ctaactaatc tgatttaaat tttcaggccc tcaagctcgt cggaggagga cacctccacg
     1561 tcaacttcaa gaccacctac aagtccaaga agccagtcaa gatgccagga gtccactacg
     1621 tcgaccgtcg tctcgagcgt atcaaggagg ccgacaacga gacctacgtc gagcaatacg
     1681 agcacgccgt cgcccgttac tccaacctcg gaggaggaat ggacgagctc tacaagccta
     1741 ggataccggt gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca
     1801 agaccgttac gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact
     1861 ccgtctacat ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag
     1921 ccatgtctat catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat
     1981 cccgtcttgc tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg
     2041 tccgtctgat ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg
     2101 ttaccaagta cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc
     2161 aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt
     2221 tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc
     2281 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     2341 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     2401 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     2461 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     2521 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     2581 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
     2641 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
     2701 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
     2761 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
     2821 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
     2881 ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt
     2941 aaatgacaga atacattccc aatataccaa acataactgt ttcctactag tcggccgtac
     3001 gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3061 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3121 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     3181 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3241 cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3301 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3361 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3421 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3481 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3541 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3601 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3661 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3721 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3781 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3841 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3901 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3961 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4021 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4081 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4141 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4201 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4261 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4321 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4381 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4861 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5041 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5101 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5161 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5221 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5281 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5341 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5401 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
