LOCUS       sequence-376435-        8201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY21
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3566..3586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3608..3628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5792..5814)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5914..5933
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6011..6029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6097..6201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6202..6270,6271..7062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6420..6439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7233..7821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7722..7741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7975..7992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8109..8130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8145..8150,8151..8168,8169..8175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8183..8199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8188..8201,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cacccacgga
      181 cttaacgttc taagtctgta ctgataaata tttattaatg gaagaaaagg caaactaaaa
      241 acaaaatcgt cgttcgtttt accacctata attttttgaa attcatttat attcaaggtt
      301 catagttcag aggatcctca aatgttgaaa aacatttttt tccaaaaatc aaaacctccg
      361 ctatcacata ctaaaaatat gctgatgtaa agttatcaat catcatcatg aatcctagaa
      421 tttaaaaatt caaaacactg atctactcac gtattctgag gtcttcttct cttcaaaaaa
      481 aaaaccttca ataatactga acgttcaagt tcacatcgcc atgcttattt gctgtgtcgc
      541 gcactccaat ttctagacgc cttcccacta aaaataagag tggagtgaga gaatgaacga
      601 tgaaaattat tccggtgaaa gaaccttgtg ttgcaaagcg gaaccaaaca aaaaatagaa
      661 cttcttaaat agaaaaagtt ctttaacgat tgattgcaac gtcatcataa gcgtcgtgtg
      721 ctctcaccgt gctcctcttt tttctctaat tccagaatgt gatggaaaga cgacctcatt
      781 cactttttcg ccttcttttc tactcttact gctcattttt tagtttgact aaatgcatgc
      841 gctggttttt gagtcaaatt tcagaataac ttccgtttcc ttttctcaat aagatatatc
      901 taatatagaa gtccaaaaaa aatttagtta tacgtcaatt attaatagaa cttgttataa
      961 ttttggatta tattacaaaa aaccatacta aggctgtaat atttacccaa gatcaatgga
     1021 atattttctc attgccacaa aaaatcacaa gccccgaaat actgtcgaaa acattttatt
     1081 agcatgcaag atattttatg gatatttttt tgcaagtatt tctgcaatac ttccaaccaa
     1141 aaatggttgc cttcgtgcct gtcattctgc cttttgaaga tcccctgtaa ttctatttga
     1201 gttttttctt ctattttttg taaaaatttc aaattttgaa acacgtgccc tttccctgta
     1261 aaactctaaa tttagctttg atctagtaag tagaaaaatt agtcgataaa ataatttaag
     1321 gaaactgtgt tggttctgaa aacggatttc aatgttttaa aatttttttc cgggaatatc
     1381 aataattttc tatatcacta aacgtcatat attcgttcaa tttaacttta atttttttca
     1441 aacccttttt gctcttgcca tttagttaaa ataaatttta ctgtaatttt caaagtagaa
     1501 atttgattgg cacactaata caggaaatgc gattacattc ctgaattatt agggttttat
     1561 tttccttgaa actatttaaa atgttcattt ttgttccaca atttcttccc atgctccgtc
     1621 caaatattta agttgttttt ggtttgttac ttttgtcctc ccgaccgctt ttcggttacg
     1681 gattataatc gagtcggaca taacccttcc ttctttgctt tcttgaacac ttcttctcat
     1741 ttttggtttt tatttataaa ctttctttcg tcatgatcat ctaaacatgt catacacaga
     1801 aagagcacac caagggcagg gttaatcatt aatttcttgg aattcttcag ctattttggt
     1861 tggaaaatga aagttgaaaa tgtttgtgcc gcttcagatt tttagaactt tagttttccc
     1921 tagaatttcc atgtttccca cgagggacac aaggcattag ttattatttt cactttccgt
     1981 gctctgtgtg gagatctgtc tttgaatgta cttaacgggt tcactttttg aggccaactt
     2041 ttttcccgtt ttcgatgaga aatttaaaaa tttacattag gaaaactctc ttacttcggg
     2101 cccaagaata aggtggatca acgtttggta agaaagtttt gctatacttg ggtttgcctg
     2161 agttttgcca acttttcatc caggttaacg aagacaaaaa aaacattaga acatacgatt
     2221 aggttaatac atctgggttt tttcaatttt ccaaaccatc tggatgagac tcctttttaa
     2281 tatattcaaa attattagtc cataacaaaa agtgcgaagt aacctaaaaa aaacacttaa
     2341 aaactcaaat gtagagacca acaaaaagtt tccagcagag tcccgtgcca tgtgattcaa
     2401 aatgccaaaa caacctattt tcggtagttc tttcaggttg ctaaatgttc caagaagttc
     2461 accagaaaga taaaaattgg aagatttttc tgtagaaaat tatattttgt agaaatgtaa
     2521 ttacttttcg acaaataaaa aagtcgtact ttctttttaa cttttccacc tgattatttt
     2581 ttttcgaaag cttatggtaa cttaaaggta tcaaaattaa agaataccaa actattgaaa
     2641 aagttgacac atttttgatt aaacacaaga caacaaaatc taatgagcag tggatgtgca
     2701 gaattgaaaa aggtatctgt caaaaaatat aataatgact cttttgtgga gtcattatta
     2761 tattttatat tcaattgttc atgtcaataa acctatatat atcagaatga ctcttattcc
     2821 tttattttaa tattataaca gtaatcccta aacataattg tttccctaat aaatgacata
     2881 attcaatgat gcgtcatgat aaaagttgcc ctaatgtcta gtcattggtt cccattttta
     2941 tttttcttat tcaatttccc cactgaaacc tgtcaattat acatgttccg tctatagaac
     3001 ccttcacttt ttccaattca tttcctcaat ttgacatcac ccggaagcct aaatcttcca
     3061 aaatctctaa aattggagca aacgaaagtc gtgaagattt agaagacgtc aggcatgtgt
     3121 ggcttctatt tatattctat ttactgtcga cctcttcctt gaaaatctgt ttaaaagttg
     3181 gcgtctcttt ctaaccagtt tatcgtttgg ttggttaacg tctcctccgg aaaatttgct
     3241 cgaacttttg tctttactcc ttttttgttg ttctgacgca tctgttcacc ccatctcaaa
     3301 gcttctcgct taccgccttt aattcaatgg tctccggatt cgcatgaatc ttgtttctcc
     3361 gtttacaccc tctcgtcttt tttctgtgtg ctctgttctt atgctcccta cgaacttaac
     3421 caacacatat ttccaaaaaa tgatttctag acccgggatt ggccaaagga cccaaaggta
     3481 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt
     3541 accgagctca gaaaaaatga ccgctccaaa gaagaagcgc aaggttccag tcgagaagat
     3601 gactgcccca aaaaagaaac gtaaggtccc gcggatgtca gagcttatta aggagaatat
     3661 gcatatgaaa ctgtacatgg aaggaaccgt ggacaaccat cactttaagt gcacttcgga
     3721 gggggaaggc aaaccgtacg agggcaccca gaccatgcga atcaaggtgg tcgagggagg
     3781 cccactgccc ttcgccttcg acattctggt aagtttaaac atatatatac taactaaccc
     3841 tgattattta aattttcagg ctactagctt cctgtacggc agcaagactt ttattaatca
     3901 cacccagggc atcccggact tcttcaaaca gtcgttccct gagggcttca catgggagcg
     3961 tgtgaccaca tacgaagacg gtggcgttct caccgctacc caggacacca gcctccagga
     4021 cggttgcctc atatataatg tggtaagttt aaacagttcg gtactaacta accatacata
     4081 tttaaatttt cagaagatcc ggggagtaaa cttcacatcc aatggtccgg ttatgcagaa
     4141 gaaaacactc ggctgggagg ccttcaccga gacgctgtat ccggctgacg gaggcctgga
     4201 aggtcgaaac gacatggccc tgaagctcgt cggcggtagc catctcatcg caaacgctaa
     4261 gaccacatat agatccaaga aaccggtaag tttaaacatg attttactaa ctaactaatc
     4321 tgatttaaat tttcaggcta agaaccttaa aatgccggga gtctactatg ttgactacag
     4381 actggaacgg atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggttgcagt
     4441 tgccagatac tgcgacctcc cgagcaaact gggtcacaag cttaatccta ggataccggt
     4501 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     4561 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     4621 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     4681 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     4741 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     4801 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     4861 cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     4921 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     4981 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     5041 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     5101 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     5161 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     5221 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     5281 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     5341 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     5401 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     5461 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     5521 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     5581 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     5641 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     5701 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     5761 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5821 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5881 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     5941 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6001 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6061 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6181 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6241 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6301 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6361 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6421 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6481 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6541 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6601 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6661 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6721 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6781 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6841 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6901 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6961 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7021 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7081 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7141 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7321 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7441 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7561 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7621 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7681 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7741 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7801 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7861 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7921 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7981 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8041 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8101 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8161 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
