LOCUS       sequence-376436-        6085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter driving panneuronal nuclear TagRFP-T expression
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY11
FEATURES             Location/Qualifiers
     source          1..6085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1689..1709
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1734..1754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1776..1796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3676..3698)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3798..3817
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3895..3913)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3981..4085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4086..4154,4155..4946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4304..4323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5117..5705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5606..5625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5859..5876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5993..6014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6029..6034,6035..6052,6053..6059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6067..6083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6072..6085,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgctgtgtct ctccgtccta
      181 tccactatct ttcatccaga ttcccggttt tcccgagaaa aagtgagaga gagaagaggc
      241 tactgcgcgg cgacactggc tgcggacacc ctcactcctc tacttgtctc catcgatttt
      301 cgctggttcg tcgaagcctc gtcgccaaca caacttcttc ttctttttcg tcctcttctt
      361 ttctgccaca cgcttgcaca gcttcttctt ctcattttac gctgtctact tttttcggag
      421 gggccatacc cccacacaca gctcctttta tttggcttct cttcttcttc ttgtccatat
      481 acttcttaaa agtctcgaac ttctctctct cactctccat caatttcttt atggctaccg
      541 cttgcgctct gccgcttccg aagaaagaag gcgttggcag agctctcaat tctatttttt
      601 ctcgccgtag gctatcgatt tctcagcttc tcttctctct tcctcactcc accaccacca
      661 cttcgggctt cttcttttct tcattttcgt cttcttcttc atcctcttct ttttttttca
      721 gttccccctc actcctcccc ttatatgcgc gtgcgagagg ggtgcaaaag cagcgcgcat
      781 ccgagaattg aaacgagaaa acggagacgc agcagcagtt cgtcctcaga aaaatagcca
      841 gtaaaaagag aaaaagatag agagagacct atcgcatttt attttcaaat tgcaattcct
      901 gcaattcatg tgtgcgtgtg cccattttca attcttcccg ttatttttca gaccattacc
      961 aacgttcttt ctagatcttg agacaatctt ccttctgctc aatcgttcgt cgtgaagacg
     1021 gcatcgacga cgacgacgta gagggccggg actcttccca ctgaaactca atacttttgc
     1081 atatttcaca ttctcttcct ccgttggttc accaaaagag aaaagggacc gacatgaaaa
     1141 ggaaacgaaa gcttaaaaga gacggaaaat cgatgtacta gagtgctcgg aagtagttga
     1201 gaggcgcaga gagagagctt tcattcagaa gacacacgac gttattcctc gcaacgcctt
     1261 cttcttttta acaatcttca atacatttta gtgaactttc ttttatttct gtttcattta
     1321 aaaatagtct taaatttcac tatatttaat ttttttcttt tattatctgg aatgtttcag
     1381 ctttcaacat gccattaaag agtgtgctcc acgagccgga gagacgcaga tgggcatgag
     1441 aagaagtcga taagaaagga gggtgccgtg ctccctcaac gtcgtctgct ccatcgccga
     1501 atttgtgctc tttcgtcgag agagctcttc actttgaact ggccaggatc cccgggattg
     1561 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     1621 ggacccttgg agggtaccgg ttttcccagt cacgacgcgt ggtaccgagc tcagaaaaaa
     1681 tgaccgctcc aaagaagaaa cgcaaagtac cgagctcaga aaaaatgacg gcacctaaaa
     1741 agaaacgcaa agtaccggtc gccgagaaga cagccccaaa aaagaagcgt aaagttccac
     1801 gcgtcatggg agtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1861 agcgcgcttc caagggagag gagctcatca aggagaacat gcacatgaag ctctacatgg
     1921 agggaaccgt caacaaccac cacttcaagt gcacctccga gggagaggga aagccatacg
     1981 agggaaccca aaccatgcgt atcaaggtaa gtttaaacat atatatacta actaaccctg
     2041 attatttaaa ttttcaggtc gtcgagggag gaccactccc attcgccttc gacatcctcg
     2101 ccacctcctt catgtacgga tcccgtacct tcatcaacca cacccaagga atcccagact
     2161 tcttcaagca atccttccca gagggattca cctgggagcg tgtcaccacc tacgaggacg
     2221 gaggagtcct caccgccacc caagacacct ccctccaaga cggatgcctc atctacaacg
     2281 tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagatcc
     2341 gtggagtcaa cttcccatcc aacggaccag tcatgcaaaa gaagaccctc ggatgggagg
     2401 ccaacaccga gatgctctac ccagccgacg gaggactcga gggacgtacc gacatggccc
     2461 tcaagctcgt cggaggagga cacctcatct gcaacttcaa gaccacctac cgttccaagg
     2521 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aagccagcca
     2581 agaacctcaa gatgccagga gtctactacg tcgaccaccg tctcgagcgt atcaaggagg
     2641 ccgacaagga gacctacgtc gagcaacacg aggtcgccgt cgcccgttac tgcgacctcc
     2701 catccaagct cggacacaag ctcaacggaa tggacgagct ctacaaggcc taggataccg
     2761 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
     2821 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
     2881 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     2941 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     3001 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     3061 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     3121 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     3181 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     3241 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     3301 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     3361 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     3421 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     3481 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     3541 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     3601 ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     3661 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3721 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3781 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3841 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     3901 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6061 gtggaattgt gagcggataa caatt
//
