LOCUS       sequence-376438-        7221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-13 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY27
FEATURES             Location/Qualifiers
     source          1..7221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2580..2600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2622..2642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4812..4834)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4934..4953
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5031..5049)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5117..5221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5222..5290,5291..6082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5440..5459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6253..6841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6742..6761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6995..7012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7129..7150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7165..7170,7171..7188,7189..7195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7203..7219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7208..7221,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcgggccca aattgtgcct
      181 cctgatgctg aatcctcaat ccatttctcc atacttcctt ttcacttcct ccatttggcg
      241 gaaggtgggc ggtcctcccg ccgaaacttg atctcttttt tatttttaag aagatccttt
      301 atgtttttta tattgacgtt tacttttttt ttggaagaaa gtgggtgaac tacaatcaga
      361 ttatttttat gctccgcgaa aatttaatca tgatcaaatg tgatatatga tgataatttg
      421 ggaataaaaa agtggaggtg gaacataatt tgtgttctta cggactataa tttatttgat
      481 ataaaacatc attatttcac ttgttgcagt caaaatgttc agacttgcaa ccgctttttt
      541 ctttaacccc atttaaatag gtcattttga aactgaaatt ttagatatca atgtaacaat
      601 tttcagaaaa tcatgaaaat gagaaaataa cactgaatct caattttttc taactagccg
      661 tttattaatt aaaactgtag tagcatttta gtcggcattt tgtgtcaaaa caaattcatt
      721 cttgtatttt ttttttaatg cagtcaatat cttagtaaat tgccaaatat acgaaaccta
      781 tggagcgttt ctgaggaatt cagaacacta gaacactgtt caatccttgc tacatttaaa
      841 tacagggtgg gccaaaagta tggtaacacg tttgcagctc tataacttta ctagtggaga
      901 gagttattac atcaatttgt acaaggtttg ttctccataa aatactgacc agatgaacgt
      961 tttgaatttt caaaatattc ccatccgcat cgctgcaggc cttcagaact tttgagatca
     1021 cgacctttat ggcacgcaag tcggcattct gcgggatttc tacatttttc tcaaggaatc
     1081 catcaagtgt cttacaagtt tttatagttc aaagaatgct cttggtctag agatttttta
     1141 aaaacaaaaa tccaaataat ttctatcacg gtatttctgt catggactgc ggcagtaggt
     1201 taagtcttga atggcatagt cgccagaaag atggatgtga gcggcgagta gcagtatgca
     1261 gaaaaattca ttttagtatt tttcgagacg ttttgacctg actttccaga atttttttag
     1321 aaaattgagc tcgaatgatt ttgtgggtcg ttacgccttg cgaaaccaaa aaaactgagt
     1381 aaatttcgca aagttgaatt catgaaaaat tcaaaggttc tggcgccagt gtcgacacag
     1441 aaaatttttg tatgctacaa aaacaatcaa aaacgcttct tcacttcttt aactttaaat
     1501 ttaacataaa aatcatacat ctgaaaatta ttggcgtgcg tgggaggtgt gtttgtgtgg
     1561 gaagtgccgc gtgccaaatt accgtttttt cgaaagggtt cgccaaattt tcgaggtgat
     1621 aaagatcgca ccttattggg aaacattaca aattttgagt caaattgaag gaaatttatt
     1681 cagctatcag tgtttgatag aagaatgtgc gtatgacgtg gcagtcgcgc gaaaagctca
     1741 tgtaaaagaa cgcttcatgt gttaccatac ttttggccca ccctgtataa atcgtgaaaa
     1801 cataattaga ttataaaagt aaaagtcgcc cggtttcgat tatggttggc aaaatctgag
     1861 taatcgctac ttttttttga ttgcaattaa aaataaattc tgacaccttt taaaaacatg
     1921 taccatgata ttttttaaac gatttcgcga cttggactac atcttttgaa ctccaatatt
     1981 atcagttccg aagtgtgatg ttgatgttaa cataatgaca tttacaacgt attctgattt
     2041 ctttgaatat cttctcgaac tctcgagaca catctgttca gattccagtt gacttgtcac
     2101 atttacgcat ttccctcctc cattctttta ttttgtttct atttcttgcg taaatctgat
     2161 taaaatcgag aagtaggcgc gcattttctg tcttcacctt ttgactataa aagtactcta
     2221 caaatattga acaacacttt ttaaaagttg tagtagttat tggccatcag gaattaggag
     2281 gtcagttgca aaatcatgca tcttttagga ttaaaagctt aaatcttgct cattcatttc
     2341 ctgtcttatt ttgaagtttt tctctcttcc aacgcctccc actgtgacta ctatcatcta
     2401 ttattatctt gtagggattc ctcaatttcc agacaccctc aaactcggcc ccgttccgat
     2461 tcgaacccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
     2521 agaacatttt caggaggacc cttgcttgga gggtaccgag ctcagaaaaa atgaccgctc
     2581 caaagaagaa gcgcaaggtt ccagtcgaga agatgactgc cccaaaaaag aaacgtaagg
     2641 tcccgcgggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagc
     2701 gcgctgtgtc aaaaggagaa gagcttatta aggagaatat gcgttccaag ctctacctcg
     2761 agggatccgt caacggacac caattcaagt gcacccacga gggagaggga aagccatacg
     2821 agggaaagca aaccaaccgt atcaaggtcg tcgagggagg accactccca ttcgccttcg
     2881 acatcctcgc cacccacttc atgtacggat ccaaggtaag tttaaacata tatatactaa
     2941 ctaaccctga ttatttaaat tttcaggtct tcatcaagta cccagccgac ctcccagact
     3001 acttcaagca atccttccca gagggattca cctgggagcg tgtcatggtc ttcgaggacg
     3061 gaggagtcct caccgccacc caagacacct ccctccaaga cggagagctc atctacaacg
     3121 tcaaggtccg tggagtcaac ttcccagcca acggaccagt catgcaaaag aagaccctcg
     3181 gatgggagcc atccaccgag accatgtacc cagccgacgg aggactcgag ggacgttgcg
     3241 acaaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggccc
     3301 tcaagctcgt cggaggagga cacctccacg tcaacttcaa gaccacctac aagtccaaga
     3361 agccagtcaa gatgccagga gtccactacg tcgaccgtcg tctcgagcgt atcaaggagg
     3421 ccgacaacga gacctacgtc gagcaatacg agcacgccgt cgcccgttac tccaacctcg
     3481 gaggaggaat ggacgagctc tacaagccta ggataccggt gggtgaagac ccaccaaagc
     3541 catctgccaa gggagccaag aaggccgcca agaccgttac gaagccaaag gacggaaaga
     3601 agagacgtca tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc ctcaagcaag
     3661 ttcatccaga cactggagtt tcctccaaag ccatgtctat catgaactct tttgtcaacg
     3721 atgtcttcga gcgtattgct gctgaagcat cccgtcttgc tcactacaac aagcgttcca
     3781 caatctcatc ccgcgaaatt cagaccgctg tccgtctgat ccttccagga gagcttgcca
     3841 agcacgccgt gtctgaggga accaaggccg ttaccaagta cacttccagc aagtaggaat
     3901 tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc
     3961 cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt
     4021 tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     4081 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
     4141 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     4201 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     4261 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
     4321 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     4381 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     4441 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     4501 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
     4561 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
     4621 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
     4681 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
     4741 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat
     4801 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4861 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4921 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     4981 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat
     5041 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5461 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5941 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6061 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6121 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6181 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6901 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6961 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7021 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7081 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7141 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7201 aattgtgagc ggataacaat t
//
