LOCUS       sequence-376439-        6191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srab-20 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY24
FEATURES             Location/Qualifiers
     source          1..6191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1556..1576
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1598..1618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3782..3804)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3904..3923
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4001..4019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4087..4191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4192..4260,4261..5052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4410..4429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5223..5811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5712..5731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5965..5982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6135..6140,6141..6158,6159..6165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6178..6191,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg caatgagcaa
      181 agaatccttg aattgcttaa actcattctt aacattcata tcgggctcaa cttttttttg
      241 gcaactcgaa tgtatacagt ctaataaagt gaacactatt aaagaatgtt atacgcttaa
      301 atgtacataa ttcgcatacc acaacacagt ggcccacgta acccataatt ttaaccgaaa
      361 attttggaaa atcctaaata gtttgatttt tattgagaca aacttctact gacattttat
      421 ttttaaaaat ttgaaagaaa aggttgaaaa ataaattttc aagactgcaa aggttgcaat
      481 tatgtattat cagtcctgac tggaaaccag ttgttagaga attttaacaa ttatttttga
      541 agaaatgtgc ctcaaccgag gtgtgcaatt catttgttct ctcacttttg aatacatgtc
      601 tgaatttttc aaaaggagag cgtattagaa gtttatgatt tttataaaga tttttatatt
      661 tttcaaaatg tttaactatt attatttgcc acatttggtg atgtagaaac atgagaactt
      721 acataaaaat aaaaataacc tttatagtgg cagatgtaaa aatgaaaaat tccaaagggt
      781 aagaactaaa ttcgagtttt ctgttctggc caacttgatc tcacccctct ccctgctcct
      841 attttacatt cttctcgtta ttatcacccg ctaaaaagag ctaaatcctt acacgtttgt
      901 gttttagact tttttcaaca agtcctagaa aggtgctaat caccaaatat gcttacgttg
      961 tgttgtgtag attacatttt tgtttctagt aaccacctga aaaaacaatt acttctaaca
     1021 tgaaaaaatg ccgtgatagt tcattagttc aaccacactt caattattgt tcactgcatt
     1081 tcctcacgca gttagccagg ggcagtagtg ggaattaaat tttttgcaga atttgtctaa
     1141 aatctgagat tttgccacgt tttctctttt gggacacagt tttttttcaa agtataagca
     1201 tttcatgcat attttttcgg actttttatt tatttttttt tttttattat ttgcaaaatt
     1261 tggttctgtt tttttgaaac attaacctac cataaaaaaa aacgttttgg taattttttt
     1321 aggatatata tatcataagc aagataccca caaattttaa atttccactt tttaattcta
     1381 cgatatacca caacaacaaa atttttaaaa aattttaaga agcgattgct aattgttcca
     1441 gcccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     1501 cattttcagg aggacccttg cttggagggt accgagctca gaaaaaatga ccgctccaaa
     1561 gaagaagcgc aaggttccag tcgagaagat gactgcccca aaaaagaaac gtaaggtccc
     1621 gcggatgtca gagcttatta aggagaatat gcatatgaaa ctgtacatgg aaggaaccgt
     1681 ggacaaccat cactttaagt gcacttcgga gggggaaggc aaaccgtacg agggcaccca
     1741 gaccatgcga atcaaggtgg tcgagggagg cccactgccc ttcgccttcg acattctggt
     1801 aagtttaaac atatatatac taactaaccc tgattattta aattttcagg ctactagctt
     1861 cctgtacggc agcaagactt ttattaatca cacccagggc atcccggact tcttcaaaca
     1921 gtcgttccct gagggcttca catgggagcg tgtgaccaca tacgaagacg gtggcgttct
     1981 caccgctacc caggacacca gcctccagga cggttgcctc atatataatg tggtaagttt
     2041 aaacagttcg gtactaacta accatacata tttaaatttt cagaagatcc ggggagtaaa
     2101 cttcacatcc aatggtccgg ttatgcagaa gaaaacactc ggctgggagg ccttcaccga
     2161 gacgctgtat ccggctgacg gaggcctgga aggtcgaaac gacatggccc tgaagctcgt
     2221 cggcggtagc catctcatcg caaacgctaa gaccacatat agatccaaga aaccggtaag
     2281 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggcta agaaccttaa
     2341 aatgccggga gtctactatg ttgactacag actggaacgg atcaaggagg ccaacaacga
     2401 gacctacgtc gagcagcacg aggttgcagt tgccagatac tgcgacctcc cgagcaaact
     2461 gggtcacaag cttaatccta ggataccggt gggtgaagac ccaccaaagc catctgccaa
     2521 gggagccaag aaggccgcca agaccgttac gaagccaaag gacggaaaga agagacgtca
     2581 tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc ctcaagcaag ttcatccaga
     2641 cactggagtt tcctccaaag ccatgtctat catgaactct tttgtcaacg atgtcttcga
     2701 gcgtattgct gctgaagcat cccgtcttgc tcactacaac aagcgttcca caatctcatc
     2761 ccgcgaaatt cagaccgctg tccgtctgat ccttccagga gagcttgcca agcacgccgt
     2821 gtctgaggga accaaggccg ttaccaagta cacttccagc aagtaggaat tccaactgag
     2881 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     2941 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     3001 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     3061 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     3121 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     3181 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     3241 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     3301 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     3361 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     3421 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     3481 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     3541 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     3601 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     3661 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     3721 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3781 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3841 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3901 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3961 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     4021 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4081 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4141 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4201 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4261 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4321 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4381 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4441 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4501 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4561 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4621 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4681 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4741 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4801 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4861 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4921 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4981 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5041 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5341 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5881 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5941 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6001 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6061 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6181 ggataacaat t
//
