LOCUS       sequence-376440-        5702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY20
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1067..1087
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1109..1129
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3293..3315)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3415..3434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3512..3530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3598..3702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3703..3771,3772..4563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3921..3940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4734..5322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5223..5242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5476..5493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5610..5631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5646..5651,5652..5669,5670..5676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5684..5700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5689..5702,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg tcgagagcag
      181 caaatcataa atttcatctc ttagaaatgt tttattatgg gaacgcagaa aaatgcgctt
      241 gttgaaatcg atgcaactga gctccactgc taaatgagaa cgatccgcct ttaaacgttg
      301 ggtctcgtta ggtattggcg acaaaaccag caattctaac gttttccatc attttcgccg
      361 atttcaacca atttttcgtc tttgtactat tttcccgttg aaattgatga aaaacctttt
      421 ttgacaaaaa atcttataaa aacaatggaa aatcaccgaa aaaacgggac tcaaagtttg
      481 ccattggagc gcgcttgcac ctcaatttca aagctctcat ttttctgcgt ttccataata
      541 tttaaaaatt ttaatcacaa atctataata tcaaaccccc ttcttcttgt ttccatgtca
      601 ttccttccaa atatgttcat agccaaaaaa tgttcactgt ttttcatctt tttaaaatgt
      661 tttttatctc cgggtttcta ttgttttctc tgttttgtta ataagaacga attttataag
      721 taaatataag taatagtagt acgtaacaca tgaaaatcct tcggaaaaac taatcaaact
      781 aatgcatatt gtacggatgg ataatctagt actgacgtat tttgagtaga cacagtagtt
      841 aatcttaaat ttaagcagct caaatcagat ttcaagtgaa gcggcaacgt ggaagagctc
      901 agttggaaga aatagatatt tcgcagctac tttgcagtcg gagcatcaga aacccgggat
      961 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     1021 gaggaccctt gcttggaggg taccgagctc agaaaaaatg accgctccaa agaagaagcg
     1081 caaggttcca gtcgagaaga tgactgcccc aaaaaagaaa cgtaaggtcc cgcggatgtc
     1141 agagcttatt aaggagaata tgcatatgaa actgtacatg gaaggaaccg tggacaacca
     1201 tcactttaag tgcacttcgg agggggaagg caaaccgtac gagggcaccc agaccatgcg
     1261 aatcaaggtg gtcgagggag gcccactgcc cttcgccttc gacattctgg taagtttaaa
     1321 catatatata ctaactaacc ctgattattt aaattttcag gctactagct tcctgtacgg
     1381 cagcaagact tttattaatc acacccaggg catcccggac ttcttcaaac agtcgttccc
     1441 tgagggcttc acatgggagc gtgtgaccac atacgaagac ggtggcgttc tcaccgctac
     1501 ccaggacacc agcctccagg acggttgcct catatataat gtggtaagtt taaacagttc
     1561 ggtactaact aaccatacat atttaaattt tcagaagatc cggggagtaa acttcacatc
     1621 caatggtccg gttatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     1681 tccggctgac ggaggcctgg aaggtcgaaa cgacatggcc ctgaagctcg tcggcggtag
     1741 ccatctcatc gcaaacgcta agaccacata tagatccaag aaaccggtaa gtttaaacat
     1801 gattttacta actaactaat ctgatttaaa ttttcaggct aagaacctta aaatgccggg
     1861 agtctactat gttgactaca gactggaacg gatcaaggag gccaacaacg agacctacgt
     1921 cgagcagcac gaggttgcag ttgccagata ctgcgacctc ccgagcaaac tgggtcacaa
     1981 gcttaatcct aggataccgg tgggtgaaga cccaccaaag ccatctgcca agggagccaa
     2041 gaaggccgcc aagaccgtta cgaagccaaa ggacggaaag aagagacgtc atgcccgtaa
     2101 ggaatcatac tccgtctaca tctaccgtgt cctcaagcaa gttcatccag acactggagt
     2161 ttcctccaaa gccatgtcta tcatgaactc ttttgtcaac gatgtcttcg agcgtattgc
     2221 tgctgaagca tcccgtcttg ctcactacaa caagcgttcc acaatctcat cccgcgaaat
     2281 tcagaccgct gtccgtctga tccttccagg agagcttgcc aagcacgccg tgtctgaggg
     2341 aaccaaggcc gttaccaagt acacttccag caagtaggaa ttccaactga gcgccggtcg
     2401 ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt
     2461 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg
     2521 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     2581 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     2641 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     2701 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     2761 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     2821 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     2881 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     2941 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     3001 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
     3061 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     3121 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
     3181 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttcctacta
     3241 gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3301 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3361 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     3421 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     3481 ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt
     3541 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3601 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3661 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3721 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3781 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3841 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3901 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3961 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4021 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4081 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4141 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4201 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4261 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4321 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4381 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4441 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4501 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4561 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4621 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4681 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4741 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4801 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4861 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4921 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4981 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5041 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5101 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5161 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5221 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5281 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5341 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5401 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5461 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5521 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5581 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     5641 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     5701 tt
//
