LOCUS       sequence-376441-        7717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  glr-2 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY90
FEATURES             Location/Qualifiers
     source          1..7717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3082..3102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3124..3144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5308..5330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5430..5449
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5527..5545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5613..5717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5718..5786,5787..6578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5936..5955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6749..7337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7238..7257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7491..7508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7625..7646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7661..7666,7667..7684,7685..7691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7704..7717,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc aatgttgccg
      181 ctaaagtaat aaatatttaa tattagagta aaaataataa gagtttttag gaagagcatg
      241 ttcgtcgagg acaactggct gatgtttgtc tagatactcc attatgtaat ggacatacaa
      301 ctggtatgga tatcctgtgg acaggaacac caatggtcac aatgccttgt gagttttcag
      361 cttttattac agagacttaa tattttgtga tctttgcgtc aaaaataccc ggttttgacc
      421 aagggtttca tcgaaatttt cgattctatt tttcgaattt tatatctaga aaaattcgaa
      481 aaaaaaaatg ctttgaaaat aaaaaagaag tcgatttttc gattttttta aaagaaaaaa
      541 taatacaatt ttaaaatcaa attttgttta tgttcaagaa caaaaatttt taattaatga
      601 gaaaaaaaca aaagaaaagt ttattttctt ctcaacaaat aatggatcaa gttttttttt
      661 ttttggaaaa ttgaatattt ttgacatatc ggaaattctg aaaatctaaa aacggaaaat
      721 ttaaactttt caaaaaatat tgttttttcg attgttgtga aaaatcgaag aatggacatc
      781 attggtcatg acacgacatt gatgaaaatt ctacactaac gtaactttga aattacagta
      841 ctctttaaaa gcgcgcgcct ttacttattt aacaaaaatt tgtcgtgtcg agaccgggta
      901 ccgtattctt accgaaaaat cactgttttt ttatgtgctt ttcattttaa tagctgagaa
      961 aatgaaataa aataaaattt cagtggaatc attggcttct cgtgtggcaa cctcccagct
     1021 ttacgctctc ggagttccag aattagttgc taaaacaagg caggaatatg tttcaattgc
     1081 agttcgactt ggaactgacg ctgatcagtg agtttatttc tagtctcaca gaggttcaac
     1141 atttttccac tttcagcttg gcgaacatgc gtgcaaaagt atggatggct cgtacctcat
     1201 caacattatt cgatgtgaaa cagtattgtc atgatatgga agatcttttg ggacaaatgt
     1261 ggaaacgata tgaaagtgga atgcccattg atcatatcac taataatacg gaaacgccac
     1321 acggcttgtg aatagatttt cgaaggattt ttaaaaaatt gtatattttg tataattttc
     1381 acaaatgatt caatgcattt catttctctt caagattttc catattggaa accaaatgat
     1441 ttttttgctc atttctttct ttctttgcgt attactcctc agtgctcttt ctacaatatc
     1501 tcgctccaaa tccccttctc caccgttcct cataatctcc attgggtaaa ttctttgtga
     1561 tttcattttg tacgaagaat tttcaatttt cccatccatc gtcctatagc cgttcttatc
     1621 aaagacataa gatccgtggt tcttgttttg tggatctctt catcactcta atcactctta
     1681 agaattttat tcctccctcc gtagtttttc ttttctaatt tatttttgtt ctataaactg
     1741 aagaaagaaa aacgtgccat tcattttcct ttctcgtgtt taatttttag tagaatttta
     1801 agttttaaga tcccctttgt taaaccatat tggtttccct ttcccgttta ctctttagtt
     1861 ttcttgaaat attcggtttt gtatgcaact tgatcactta ctcaattcct tctttttctt
     1921 gattttgctt tcccgtttta ataaatttct aaaaacatac ctaatgtaaa ttaaattcaa
     1981 taatgaaaat aataattatc ataaatggaa aaagaaacac ctctactgaa aatcgacttg
     2041 tgggaagtag aagtgttacc gacaagagac aacaatagta atacttctgt tcaaattttc
     2101 ccatatgtac gttctgttat caaaaacgta caagcagaaa caactcacct tctcgggcaa
     2161 ttaataaaaa taattccaac cttttttttt tgatggcatg tgcaattaga atgcatctag
     2221 tgaggtaggc cacagtatat ccaattttaa agtgacatag agatagtggg aatctcgtct
     2281 aaatacactc atatagtcta atatgacata ataaaacact acacaaattt tcagtcaacg
     2341 ttctggtaat atgccaaatt ttccaaaaat agtccaagac tttttgagat cttataaata
     2401 tgaaaattga gtcaacttct gttttccatc taactggtga cagattattt ggtatattca
     2461 cttttttttg aaaactctta caatgatgat ccttaaatga tccttaatct gaaagggttt
     2521 catgtaaaaa tcaacggctc tcatcgactt ttaattccat attttccttt cattttcaat
     2581 ccaactctat cattttcaca tcatccccaa aaccttctct tttccaacca atttaaccgt
     2641 ggggacgaat ggatgggtcc tccggaaaac gaggcagaaa attgaaaaag tggtgctcct
     2701 cctcttcttt caccccttct gtgttccaga gaatccgcgc aagaagcaat ttgagaagca
     2761 ggagaaagta agagaagaag aagaagaaga ctacgaagag tagaaagaag aagaagctgt
     2821 cattttcaat tttgggaata gacgactccg cagcgactcc tttaactcat tttctaacct
     2881 actcgtctca ttttgccaat ttacgaaatg aaatgagaat tatattttaa actggtatga
     2941 agcagagtta actctgtaaa aagcgaaccc gggattggcc aaaggaccca aaggtatgtt
     3001 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg gagggtaccg
     3061 agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga gaagatgact
     3121 gccccaaaaa agaaacgtaa ggtcccgcgg atgtcagagc ttattaagga gaatatgcat
     3181 atgaaactgt acatggaagg aaccgtggac aaccatcact ttaagtgcac ttcggagggg
     3241 gaaggcaaac cgtacgaggg cacccagacc atgcgaatca aggtggtcga gggaggccca
     3301 ctgcccttcg ccttcgacat tctggtaagt ttaaacatat atatactaac taaccctgat
     3361 tatttaaatt ttcaggctac tagcttcctg tacggcagca agacttttat taatcacacc
     3421 cagggcatcc cggacttctt caaacagtcg ttccctgagg gcttcacatg ggagcgtgtg
     3481 accacatacg aagacggtgg cgttctcacc gctacccagg acaccagcct ccaggacggt
     3541 tgcctcatat ataatgtggt aagtttaaac agttcggtac taactaacca tacatattta
     3601 aattttcaga agatccgggg agtaaacttc acatccaatg gtccggttat gcagaagaaa
     3661 acactcggct gggaggcctt caccgagacg ctgtatccgg ctgacggagg cctggaaggt
     3721 cgaaacgaca tggccctgaa gctcgtcggc ggtagccatc tcatcgcaaa cgctaagacc
     3781 acatatagat ccaagaaacc ggtaagttta aacatgattt tactaactaa ctaatctgat
     3841 ttaaattttc aggctaagaa ccttaaaatg ccgggagtct actatgttga ctacagactg
     3901 gaacggatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt tgcagttgcc
     3961 agatactgcg acctcccgag caaactgggt cacaagctta atcctaggat accggtgggt
     4021 gaagacccac caaagccatc tgccaaggga gccaagaagg ccgccaagac cgttacgaag
     4081 ccaaaggacg gaaagaagag acgtcatgcc cgtaaggaat catactccgt ctacatctac
     4141 cgtgtcctca agcaagttca tccagacact ggagtttcct ccaaagccat gtctatcatg
     4201 aactcttttg tcaacgatgt cttcgagcgt attgctgctg aagcatcccg tcttgctcac
     4261 tacaacaagc gttccacaat ctcatcccgc gaaattcaga ccgctgtccg tctgatcctt
     4321 ccaggagagc ttgccaagca cgccgtgtct gagggaacca aggccgttac caagtacact
     4381 tccagcaagt aggaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt
     4441 caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg
     4501 agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact
     4561 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     4621 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     4681 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     4741 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     4801 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     4861 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     4921 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     4981 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     5041 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     5101 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     5161 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     5221 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     5281 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     5341 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     5401 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     5461 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     5521 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5581 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7501 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7621 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatt
//
