LOCUS       sequence-376442-        7604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gpa-6 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY97
FEATURES             Location/Qualifiers
     source          1..7604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2969..2989
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3011..3031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5195..5217)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5317..5336
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5414..5432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5500..5604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5605..5673,5674..6465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5823..5842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6636..7224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7125..7144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7378..7395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7512..7533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7548..7553,7554..7571,7572..7578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7586..7602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7591..7604,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcgcggccg catccgtact
      181 aaaaagaaac aaagcaaaaa taaaatcact aaggttttgc aaggtttttt ttcattactt
      241 aaaatattat tgtcacaaaa atatacaaat ttccggaaag aaattttaaa ataacattga
      301 ttaactcatg aaattgaatt gttctgaatt ttaattaatt ttcagttttt ttgaacaaga
      361 aatgttttat cgtataatta ttaaaattaa ttgaaaaatt ctacttttgt agtaaaatag
      421 cttaaagttt gaaaaatttc aaaacttggc aacatgctaa aaaaggcaaa acagttttta
      481 ctcattttta gtatatttcg acagaattcc cactagctca gcaccctact tgtgactagc
      541 cgcgactata cctcctttta aaattcatat tatggaaaac ggttttcaaa aatttacata
      601 gtttagtggt tagactttga cccttctata aagtaaatct tactgtaatt ttcgcgttta
      661 catttattga cattcttcat attcacgtta ttatgtattt ggcgagtcca catgtgtaca
      721 tttactctag ttattgcctt cgaaagtctc cacctccccc cttatcagag tttcgcgcct
      781 ttacccgaaa ctccgttttt caattagcaa tggtgtgtcc aacgctagtc ttttctcatt
      841 tttcatcttc aaactgctaa aatcgacaat tgagagcaaa caaaattgtc gaaaatcggt
      901 gagttgtagc ttattggaat acaaattaca gtaagatata ttatacgcta caaaacacat
      961 gcctatgtga gaaaaaatac gattagatca gacacggctt gaatgaattt tgaaatagtg
     1021 aactgataag tttagacact aacgtctaat aaaaaaggtt ttaaagcaag ctgcagaata
     1081 attttgcgat aactaagtat acaaggaaac agcaacatag tgagaagttc tagtaaagat
     1141 gtagtcgaag aaagcgtatt gtctatttaa acaatacggt agaaggaatt actgcacgta
     1201 tatctcaatt actatgcgta cgttctgaac ttttcatact ttttgtttta tattctgaag
     1261 tacggttgag taccgtaaaa tagtatagtc cgacaaatga tagtcctact aaatttcact
     1321 aaaagcatgt cactttctca cttgatgtaa agtttcaacc acaatcagaa taaagtttca
     1381 ttattttttt gaactgatgg aaattgtagg gttttctaaa atatatttta aacactgtac
     1441 tttaggttta ctgtatctat gaacagaaac acactctaaa actttgaagt gattttttaa
     1501 atagttactg aaatgtttaa actaaccttt aaaagtgagc aaaaacaagg agagcatcga
     1561 aattcgcatg atgttggtga atgtcaatgt cagaataagg gaatttgtgt ggcggaccgc
     1621 gaggccgtac cctagtgtct catggcgacg gttgacgttt ttatggcaaa aaacatccac
     1681 gcggcatatg cagcggaata tagaataaaa tacacaaaaa aaaacataaa aacgtaaaaa
     1741 tatgaaaatt gttatggata cgactttcaa atcgaatctt atgattcgta gctattgtgc
     1801 ctaaaacaaa aaaaacgcag ttactcagga cagcaaagtg atcgttaaca cagttgtaaa
     1861 aaattcgaaa acatatctcg tgacgggatt attatcaaaa tgaagtttga tagatcttaa
     1921 aaatttctga attgacgacg taaaatttaa ctcattatgc tggttatcaa agaaaaatac
     1981 tcgaacgtgt tcgttttttt gtttaaaaaa aacatttatt taaaaaaacc tctatataaa
     2041 aaaatgtgtt tgaatagctt tacctggcat tcttttaaat tgatattggt tttaatgaac
     2101 tgtatatttt ccccctagcc ctcaatgttt tatattgcaa aacagaaaat aagtattttc
     2161 ctgaatacct ccatcaattt tcaagttgcg tcaacctcca gatttaatcc cgaattgttt
     2221 ttttttacct ataaaatttg taatctgtga atgcatactt ttgaactgaa gtgccgactt
     2281 ttataggaaa aagatcctta attttcatat gttactcttt atctataata tttattttaa
     2341 ctcttgaatg agggatgaaa ctagttctaa taacttttta ccaattggat aaactttaac
     2401 atttgacgac tcaaaagcct tcagagtata ataatgtgta ttcatttttt aaaatgtttt
     2461 ctgagtttaa gtttttacac tttttaatga gagatcaatt tttaaaagta agaactgcgt
     2521 aacttctttt acaactgtaa actcttgttt ttttgtttcc agttttaaat atgattttag
     2581 acgattctag aaatataatg tttttgtatt atatactgct tgcttctttt gtttattaat
     2641 tttttgtatt tttccaattt gtttaaacaa ttttctcagt tgaatttttt atataacgcg
     2701 tttatagttt tctgattact tggatttgtt aactctcttc tttgattttt tttccatgtt
     2761 atctgtttat tttttcgctt cagcgtttag atttcgccta actcattccg tctctttcgt
     2821 ttcaggtgta ttgactttca atgttatttt gaaggatccc cgggattggc caaaggaccc
     2881 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggag
     2941 ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag cgcaaggttc cagtcgagaa
     3001 gatgactgcc ccaaaaaaga aacgtaaggt cccgcggatg tcagagctta ttaaggagaa
     3061 tatgcatatg aaactgtaca tggaaggaac cgtggacaac catcacttta agtgcacttc
     3121 ggagggggaa ggcaaaccgt acgagggcac ccagaccatg cgaatcaagg tggtcgaggg
     3181 aggcccactg cccttcgcct tcgacattct ggtaagttta aacatatata tactaactaa
     3241 ccctgattat ttaaattttc aggctactag cttcctgtac ggcagcaaga cttttattaa
     3301 tcacacccag ggcatcccgg acttcttcaa acagtcgttc cctgagggct tcacatggga
     3361 gcgtgtgacc acatacgaag acggtggcgt tctcaccgct acccaggaca ccagcctcca
     3421 ggacggttgc ctcatatata atgtggtaag tttaaacagt tcggtactaa ctaaccatac
     3481 atatttaaat tttcagaaga tccggggagt aaacttcaca tccaatggtc cggttatgca
     3541 gaagaaaaca ctcggctggg aggccttcac cgagacgctg tatccggctg acggaggcct
     3601 ggaaggtcga aacgacatgg ccctgaagct cgtcggcggt agccatctca tcgcaaacgc
     3661 taagaccaca tatagatcca agaaaccggt aagtttaaac atgattttac taactaacta
     3721 atctgattta aattttcagg ctaagaacct taaaatgccg ggagtctact atgttgacta
     3781 cagactggaa cggatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggttgc
     3841 agttgccaga tactgcgacc tcccgagcaa actgggtcac aagcttaatc ctaggatacc
     3901 ggtgggtgaa gacccaccaa agccatctgc caagggagcc aagaaggccg ccaagaccgt
     3961 tacgaagcca aaggacggaa agaagagacg tcatgcccgt aaggaatcat actccgtcta
     4021 catctaccgt gtcctcaagc aagttcatcc agacactgga gtttcctcca aagccatgtc
     4081 tatcatgaac tcttttgtca acgatgtctt cgagcgtatt gctgctgaag catcccgtct
     4141 tgctcactac aacaagcgtt ccacaatctc atcccgcgaa attcagaccg ctgtccgtct
     4201 gatccttcca ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa
     4261 gtacacttcc agcaagtagg aattccaact gagcgccggt cgctaccatt accaacttgt
     4321 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     4381 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     4441 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     4501 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     4561 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     4621 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     4681 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     4741 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     4801 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     4861 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     4921 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     4981 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     5041 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     5101 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     5161 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5221 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5281 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     5341 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5401 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5461 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5581 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5641 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5701 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5761 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5821 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5881 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5941 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6001 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6061 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6121 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6181 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6241 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6301 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6361 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6421 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6481 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6781 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7381 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7441 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7501 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7561 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
