LOCUS       sequence-376445-        8116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-8 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY83
FEATURES             Location/Qualifiers
     source          1..8116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3517..3537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5707..5729)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5829..5848
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5926..5944)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6012..6116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6117..6185,6186..6977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6335..6354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7148..7736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7637..7656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7890..7907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8024..8045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8060..8065,8066..8083,8084..8090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8098..8114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8103..8116,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tcttcgtgat
      181 ggagttcgca gcaatgagtg ctcaaatgga gtctgcatga aaatgatttt ccaggaaaaa
      241 agcggtgaga tagatcaaaa ttaattgttt attttatttt ctttttactt attttaggaa
      301 tcaatcatgt ttggagaagt tgcataccaa atgcaaaaga acaaatccga tcagactgta
      361 caaaaattac tagtggagaa ggtatgtaat attcaaattg ttccatataa taaaaaaaaa
      421 atatatatat aactttcagg aaaccttgaa gtttgcacat gcgatggtaa tttatgcaac
      481 aatgacgtca atttaaatct aatactaata attatgtttt ctgcagctgt gttattatta
      541 tgatgagttg ttcattaaac ctacatatcg gaatcaaatc aaacttagat ctctattctt
      601 ttcattttcc atcggtttta tatagaactt ttgccgaagt attttgattg cataccaaat
      661 aaatgttttt acgttgttgg aaatgtttat taaaatttaa aggaacacag aatagccaaa
      721 gtgtttttaa tttccaaatt tttttttact cctcgaacag tacccttgaa cttcagctcg
      781 ctattttgaa tattcctgtc acgaagtcgg tgaatacaaa gattcactcg ctcacgactt
      841 attaataaat ctaaggattt attaacaaat gattaaatgt aaagattgat tcattgttat
      901 tacgtaacac ttgctcttca acactcaaca tcatctcttc aatcaacggc aacaaccatg
      961 cagaaggacg gcgtcagaaa ccccgattca aactaccgcc aaaaggacga ttcaaggcgc
     1021 atgaggtctc gccacgacac atacaacaac gactgcgacc gtactcttgt catcatggcg
     1081 cagcgcacat tggcctatat cgtcagtctt catcggtctt caccaccgca gcacgtgccc
     1141 gacggtggca cgtttaaatg aatgttgagt gttagtgatt caaggttttc cccttgaatc
     1201 actcgtaata aatgagttaa ttaaataaga gtcttttgtt agttcctatc agtacctcat
     1261 gggccggaaa ccactccgaa acaattccca taattttatt gttttccatt ttccaggaaa
     1321 aaaaacagtt ctttgacatt ttcaagccaa aaactatgtg tgttccgtct taagaccatg
     1381 cattggaaat tacgttcgtc actatttttg tgtgtatgtg ttttggattc ctcttctcac
     1441 atttgcggtc acaacagtat cgttcatcgg tttttatgtt gtgataaaat agagaaaaag
     1501 taaaacgagc atatttttga tagcggaaga aaaattttga gattacatat aacaaagcgg
     1561 aaagtgatta tcaaatgtca aattatcaaa taatttggaa gcttttcaat tcaatataca
     1621 gggttgattt tcaataggtg gtgcaagtaa aaaagaaata ctgtatttat atttaaagat
     1681 aaatacattt tgtttaaaca cgtaaatatt gtttcaagct ggccgcatat caaaataaaa
     1741 ccgtacaaaa actttccagt ttttcttaat tttcagtcaa ggttttagta aaatagcgag
     1801 gcgacgaaaa tatataactt tccgagaagt tcagagcagt taataattat tatcaacttt
     1861 ttacagaatg gtttacattt tttcaacatt aattatagtt aaaggacaat acatttttca
     1921 aaatattcac tgtaaatatg aatgaatact tttggaaaat aatcaaaaag ctgactgact
     1981 actgttaaac aatggcggag tgataaacta gcaaattcac ttattatgca cataattata
     2041 atgcaggata agatgactga ctttcaagac attcactatt taatgattag acttccattt
     2101 ttgaccatcc cattttgaca taagaactcc ttgatatttt gttctctttg aaaagtcttg
     2161 ttttactcta ctttaaatga attcattttc gtcatggacg atctcttcaa aaagtatgtg
     2221 ggcaccttta ctttgaatac acgcaatctt caccgtagtt tcatgttgct cgaatgaata
     2281 ttcaatgatt ggagggcaag atttatgaag agattgccgt gaaatcattt tgcgaggcga
     2341 atcatcgagg aagcaactga ttcgtgatga ataatttgcg cggtggataa gtttttggtt
     2401 tcaaagtacc cccccccccc ccccgagaag aggtggaaaa aagttgaaga attagatgaa
     2461 ggagaagtcc aagtgttacg tcaacaattt acaaatgcat agagttggta taattagttt
     2521 cggagggtgt cggtggctaa atttatctgt tagttacctc ccagcatgaa ttccgtgtat
     2581 tcactgtgtt cagtccctcg actgctcggt tatacagctg atagatagac gaggaagaag
     2641 aaaacacaca aaaacgtaaa gtgggcctct ttaaaaaaat ttaaaaaaca aaataaggac
     2701 acaaaaatat aaatatgcat tgttttcatt tttcaaaacg agggtagctt cgagaaattt
     2761 ccattttcaa atcaaacgaa ttgctacaac aatgtcaaaa tgttaaaatt tttcttggtg
     2821 gtactgtatg actctaaagt ttgatctgga aaaatttgtt ttttcgtaga taacgtttct
     2881 tacctaaaaa gcggttttta acctgatagt tgagataacc tggtttatac aataaatttt
     2941 caaatgagat aatttttaat aactttaaaa aaaaattttt ttatatcgtt ggttttcgaa
     3001 acttgatttt cagcaaattt ttaaaatttg aatcaacttg ctctcaaaga cacgtttatt
     3061 gtcttacgac ttttaaaacc ggagtaaatt taatttttcg atgaatcaat ttcagataaa
     3121 tctcagaaca agcactttac tgtttcttac ttgttttatg caatttacca tttcaagttt
     3181 tctatgcccc cgcgttcttt taccaaacgc cttccagtgg acactctaga atttcttcag
     3241 cacaagaagg cgtggcctct gaaaaggcgg agcaaagtgc gcggagccca agttagtgga
     3301 gaagacggac cagtatataa gccacgagca gtgctcagtc cacacttttc aagtagaaaa
     3361 cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac
     3421 attttcagga ggacccttgc ttggagggta ccgagctcag aaaaaatgac cgctccaaag
     3481 aagaagcgca aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg
     3541 cgggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagcgcgct
     3601 gtgtcaaaag gagaagagct tattaaggag aatatgcgtt ccaagctcta cctcgaggga
     3661 tccgtcaacg gacaccaatt caagtgcacc cacgagggag agggaaagcc atacgaggga
     3721 aagcaaacca accgtatcaa ggtcgtcgag ggaggaccac tcccattcgc cttcgacatc
     3781 ctcgccaccc acttcatgta cggatccaag gtaagtttaa acatatatat actaactaac
     3841 cctgattatt taaattttca ggtcttcatc aagtacccag ccgacctccc agactacttc
     3901 aagcaatcct tcccagaggg attcacctgg gagcgtgtca tggtcttcga ggacggagga
     3961 gtcctcaccg ccacccaaga cacctccctc caagacggag agctcatcta caacgtcaag
     4021 gtccgtggag tcaacttccc agccaacgga ccagtcatgc aaaagaagac cctcggatgg
     4081 gagccatcca ccgagaccat gtacccagcc gacggaggac tcgagggacg ttgcgacaag
     4141 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca ggccctcaag
     4201 ctcgtcggag gaggacacct ccacgtcaac ttcaagacca cctacaagtc caagaagcca
     4261 gtcaagatgc caggagtcca ctacgtcgac cgtcgtctcg agcgtatcaa ggaggccgac
     4321 aacgagacct acgtcgagca atacgagcac gccgtcgccc gttactccaa cctcggagga
     4381 ggaatggacg agctctacaa gcctaggata ccggtgggtg aagacccacc aaagccatct
     4441 gccaagggag ccaagaaggc cgccaagacc gttacgaagc caaaggacgg aaagaagaga
     4501 cgtcatgccc gtaaggaatc atactccgtc tacatctacc gtgtcctcaa gcaagttcat
     4561 ccagacactg gagtttcctc caaagccatg tctatcatga actcttttgt caacgatgtc
     4621 ttcgagcgta ttgctgctga agcatcccgt cttgctcact acaacaagcg ttccacaatc
     4681 tcatcccgcg aaattcagac cgctgtccgt ctgatccttc caggagagct tgccaagcac
     4741 gccgtgtctg agggaaccaa ggccgttacc aagtacactt ccagcaagta ggaattccaa
     4801 ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg
     4861 tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc
     4921 atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc
     4981 tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct
     5041 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag
     5101 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
     5161 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa
     5221 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     5281 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
     5341 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
     5401 aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag
     5461 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
     5521 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
     5581 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
     5641 actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     5701 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     5761 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     5821 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5881 gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc
     5941 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6001 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6061 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6121 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6181 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6241 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6301 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6361 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6421 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6481 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6541 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6601 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6661 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6721 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6781 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6841 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6901 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6961 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7021 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7261 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7921 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7981 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8041 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8101 tgagcggata acaatt
//
