LOCUS       sequence-376471-        9796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: SYMPK
FEATURES             Location/Qualifiers
     source          1..9796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4753..4777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4902..4943
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5398..5415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5404..5628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5761..5780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5912..5959
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5967..6989
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5967..6989
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7119..7240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7210..7229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7289..7305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7289..7305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7302..7324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7313..7329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7337..7343,7344..7361,7362..7367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7382..7403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7520..7537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7691..8279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7771..7790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8450..9241,9242..9310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9073..9092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9311..9415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9490..9509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     1321 ccatgaccca gctctacaag gtggccctgc agtggatggt aaagtcacgg gtcattagcg
     1381 agctacagga ggcctgctgg gacatggtat ctgccatggc gggggacatc atcctgctat
     1441 tggactctga caatgacggc atccgcaccc acgccatcaa gtttgtggag ggcctcattg
     1501 tcaccctgtc accccgcatg gctgactcag agataccccg acgccaggag catgatatca
     1561 gcctggaccg catccctcgt gaccacccct acatccagta caacgtgcta tgggaagagg
     1621 gcaaggcagc cttggagcag ctgcttaagt tcatggtgca ccctgccatc tcctccatca
     1681 acctgaccac agcgctgggc tcccttgcca atatcgcccg ccagagaccc atgttcatgt
     1741 ctgaggtgat ccaggcctat gaaactctgc atgccaacct gcccccgacg ctggccaaat
     1801 cgcaggtgag cagtgtgcgt aagaatctga agctgcacct gttgagtgtg ctgaagcacc
     1861 cggcttcctt ggagttccag gcccagatca ccaccctgct ggtggacctg ggcacacctc
     1921 aggccgagat cgcccgcaac atgccgagca gcaaggacac ccgcaagcgg ccccgcgatg
     1981 actcggactc cacactcaag aagatgaagc tggagcccaa cctgggggag gacgatgagg
     2041 acaaagactt ggagccaggc ccatcgggga cctcgaaggc ctcagcgcag atctccggcc
     2101 agtcagacac ggacatcaca gctgagttcc tgcagcctct gctgacgcct gataatgtgg
     2161 ctaatctggt cctcatcagc atggtgtacc tacccgaggc catgccagcc tccttccagg
     2221 ccatctacac ccccgtggag tcagcaggca cggaagccca gatcaagcac ctggctcggc
     2281 tcatggccac acagatgaca gctgccggac tgggaccagg tgtagagcag accaaacagt
     2341 gcaaggagga gcccaaggag gagaaggtgg tgaagacaga gagcgtcctg atcaagcggc
     2401 gcctgtcagc ccagggccaa gccatctcgg tggtgggttc cctgagctcc atgtcccccc
     2461 tggaggaaga ggcaccgcag gccaagagga ggccagagcc cattatccct gtcactcagc
     2521 cccggctggc aggcgctggt gggcgcaaga aaattttccg tctcagcgac gtgctgaagc
     2581 cccttaccga tgcccaggtg gaagccatga agctgggcgc tgtgaagcgg atcctgcggg
     2641 ctgagaaggc tgtggcctgc agcggggcag cccaggtccg cataaagatc ctggccagcc
     2701 tggtgacaca gttcaactcg ggcctgaagg cggaggtcct gtccttcatc ctggaggatg
     2761 tgcgggcccg cctggacctg gccttcgcct ggctctacca ggagtacaac gcctacctgg
     2821 ccgcaggtgc ctcgggctcc ctggacaagt atgaggactg cctcatccgc ctgttgtctg
     2881 gcctgcagga gaaaccagac cagaaggatg ggatcttcac caaggttgtg ctggaggcgc
     2941 cactcatcac agagagtgcc ctggaggtgg tccgcaagta ctgcgaggat gagagtcgca
     3001 cctatctggg catgtccaca cttcgagacc tgatcttcaa gcgcccgtcc cgccagttcc
     3061 agtacctgca tgtcctcctc gacctcagct cccatgagaa ggacaaggtg cgctcccagg
     3121 ccctgctgtt catcaaacgc atgtatgaga aggagcagct gcgggagtat gtggagaaat
     3181 ttgccctcaa ctacctgcag ctcctggtgc accccaaccc accgtctgtg ctgtttggag
     3241 ctgacaagga cacagaggtg gcagcaccct ggacggagga gacagtgaag cagtgtctgt
     3301 acctctacct ggccctcctg cctcagaacc acaagctgat ccacgaactg gcggccgtgt
     3361 acactgaagc catcgccgac atcaagcgga cggtgctgag ggtcattgag cagccgatcc
     3421 gaggaatggg catgaactcc ccggagctgc tcctgctggt ggaaaattgt cccaagggag
     3481 cagagacact ggtcacgaga tgtctgcaca gcctcacaga caaagtccca ccctccccag
     3541 agctggtgaa gcgggtccgg gatctctacc acaagcgact gccagacgtc cgcttcctca
     3601 tcccggtgct caatgggctg gagaagaaag aggtgatcca ggccctgcct aaactcatca
     3661 aactcaaccc catcgtggtg aaggaagtct tcaaccgcct gctgggcacc cagcatggtg
     3721 agggaaactc agccttgtcc ccgctgaacc ctggagagct cctgatcgca ttacacaaca
     3781 ttgactccgt gaagtgcgac atgaaatcca tcatcaaagc caccaacctg tgctttgcgg
     3841 agcggaacgt gtacacgtca gaggtgctgg ccgtggtgat gcagcagctg atggagcaga
     3901 gccccctgcc catgctgctc atgaggaccg tcatccagtc cctgaccatg tacccccgcc
     3961 tggggggctt cgtcatgaac atcctgtccc gcctcatcat gaagcaggtg tggaagtacc
     4021 ccaaggtgtg ggagggcttc atcaagtgct gccagcgcac aaagccccag agcttccagg
     4081 tcatcctgca gctgccgccc cagcagctgg gagccgtctt tgacaagtgc ccagagctcc
     4141 gggagcccct gctggcccat gtccgctcct tcacccccca ccagcaagct cacatcccta
     4201 actccatcat gaccatcttg gaggccagcg gcaagcagga gccagaggcc aaggaggcgc
     4261 ctgcggggcc cttggaggag gatgatctgg agcccctgac cttggccccg gccccagcac
     4321 cccggccccc tcaggacctc atcggcctgc gactggccca ggagaaggcc ttaaagcggc
     4381 agctggagga ggaacagaag ctgaagccgg gaggagtggg agccccctcc tcttcctccc
     4441 cctctccctc tccgtcggcc cggccaggcc cgcccccgtc tgaggaagcc atggatttcc
     4501 gggaggaggg gcctgagtgc gagaccccgg gcatcttcat cagcatggat gacgactcgg
     4561 ggctgaccga ggccgcgctg ttggactcta gtctcgaggg ccccctaccc aaggagacgg
     4621 cagcgggcgg gctgaccttg aaggaggagc ggagccccca gaccctcgca cctgttggag
     4681 aagatgctat gaagactccc agcccggctg ccgaggacgc cagggaaccc gaggccaagg
     4741 ggaacagctt ggacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     4801 gctcgagtct agagggcccg cggttcgaaa ccagcggagg cggcggaagc gggggaggag
     4861 gcagcggcgg aggcggcagc gggggcggcg gaagcgctac cggtaagcct atccctaacc
     4921 ctctcctcgg tctcgattct acgcgtaccg gtatggcttc taactttact cagttcgttc
     4981 tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacgggg
     5041 tcgctgaatg gatcagctct aactcgcgat cacaggctta caaagtaacc tgtagcgttc
     5101 gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaagtggcaa
     5161 cccagactgt tggtggtgaa gagcttcctg tagccggatg gagatcttac ttaaatatgg
     5221 aactaaccat tccaattttc gccacgaatt ccgactgcga gcttattgtt aaggcaatgc
     5281 aaggtctcct aaaagatgga aacccgattc cctcggccat cgcggccaac tccggcatct
     5341 acggaggtgg aggtggatct cgtacgggtt agtaatgagt ttaaacccgc tgatcagcct
     5401 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5461 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5521 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5581 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5641 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5701 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5761 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5821 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5881 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     5941 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     6001 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     6061 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     6121 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     6181 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     6241 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     6301 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     6361 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     6421 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     6481 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     6541 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     6601 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     6661 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     6721 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     6781 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     6841 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     6901 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     6961 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
     7021 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7081 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7261 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8101 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8761 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9421 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     9481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9541 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9601 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9661 acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa aagctttgca
     9721 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9781 gaaaggagtg ggaatt
//
