LOCUS       sequence-376486-        6315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the sequence for  the ATOH1 gene (human).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Zhang Q, Agius SC, Flanigan SF, Uckelmann M, Levina V, Owen BM, 
            Davidovich C
  TITLE     PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an
            allosteric activation of catalysis.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4592. doi: 10.1038/s41467-021-24866-3.
  PUBMED    34321472
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1):4592. doi"; pages: " 
            10.1038/s41467-021-24866-3"
COMMENT     Sequence Label: ATOH1_pUC18
FEATURES             Location/Qualifiers
     source          1..6315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4690..4707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4690..4706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4915..4934)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5034..5056
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5094..5112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5180..5284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5285..5353,5354..6145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5503..5522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aattcgagct cggtacccgc
     1021 ggagtttcct aaaagacgcc aacgttctcg cgatcggatg cacataactg ggtatgttta
     1081 tacaatatat aaaccttaaa gttccttgtt ttctaacaat tccatagtca aaagttatcc
     1141 actttggaag cactaatttt gcgtttcatt gtgcattttg gaaaacaact tcggaagact
     1201 aaatctccca cacttgagtg cgcaacacac aaggatggat gcccagggcg taaaataaat
     1261 taaacccaaa ccagtgacag agaagatgcg acgttttagc agcgtattgg cagcagggaa
     1321 ctagaataat gatgccatat acgggtacct acaattatac cgtatctatc taaaggggga
     1381 gcagaaggtg ggcggaaagg acacaatttt aacagaaagt tttcatcaaa caatatggac
     1441 cgtaggaagt ttggaacagc ggcggcattt gcaaaccatc aaatgtagct gccatctgaa
     1501 gccaagtcgt tgctaaacaa cgaccatcgc agagcaaaaa ttaaagggtg cggggatctt
     1561 taaccgcggg gcttcccgcg cactgggaaa gggcagtttt gtttctgtct cagttttcag
     1621 gcttctgtca ccttcctaac ttgcctcatc cgagtcactg taatgggaat ggggggaaaa
     1681 ttccccgtcg cttctgtggg accgaggcga agttttgctg ttttcctcct gcactggctg
     1741 caagatgctc ccggggagag aaggagacaa attcttttgc gccatcatcg ctgtcagggc
     1801 gctgtcctcg aaagtcgaga agtgcagagc gtccagctgc accgagtacc ctcccgcaga
     1861 agctggggct gagaagcgag tccggcaact cccgggcggg gtcggccgct ggctccctcc
     1921 ggaagcctgc tgagccccag ctgcggtcgc gttgcccgcg cccccttcat aggaggccgc
     1981 ggtgcgaagg tggtggtggt cgcttttgca ggaggctgga ggcggcggtg gctgttcccc
     2041 tccgctgggc gtttgtagca gctcggacaa ggcgttgatg tagatttggg ccatctgcag
     2101 ggtctcatat ttggacagct tcttgtcgtt gttgaacgac gggataacat tgcgcagctg
     2161 gtcgaaggcg tggttcagcc catgcatcct gcgccgctcc ctggcgttgg ctgctagccg
     2221 tctctgcttc tgcaccccat tcacctgttt gctggaaggg gcccgttggc ggctgcagcc
     2281 cagctcgtct accaccaccc cgcctttcag cttgcacagc tgttcccgca ctttcaccgg
     2341 cccggggctc ttgctgctgc tggcaccgcc gctgctcctc cttaccagct ccccccggcc
     2401 gtccacctcg tcccggggcg cagcggcctc tgaggcaccc agctccgggg aatgtagcaa
     2461 atactgggcg gcgcgtgccg tgcagatgcc ctgcaaagtg ggagccagcc aggcgcgtgg
     2521 gtcggtgctg tccaggaggg acagctcagg cgggtagacg ggatgctctc tcgcctgcaa
     2581 agttgcaggt ggctgcggcg gcggcggcgg ttgcgggaga tgatgcggct ggggctggcg
     2641 atggtggtct cccaactcct tcacttcagc ccactcttct gcatgcagca ggcgggacat
     2701 tgcactgcaa aggctcggac gctcaggatc ggaggcagca ctgggacacc agctcaacca
     2761 aaaaaaaggc cctttccggg tctcttctgc aacgtcttat ttttttttcc ctcctcgacc
     2821 ctcccccacc cactcggaga tcacacacca ggtcgcgtgc aactatgctt ctccggttgc
     2881 tgaaggcgct gcgccccttt aaaaagcggc acgcgccact cagtctcccc ccgctcctct
     2941 cccaaccccc tcctcgcgcc ggtcgcgtgt cttctcagcc aatggagggc gcgggcaggc
     3001 gcgtgcgagc agccaatcaa agagttttta caccccgaga aaagttcttg tgtgcagagg
     3061 actcccaagc ctgctcaggt tttttttttt tttccttctt ttaaattcgt cagattcata
     3121 tgttaattgc agggctgtaa ttcgactccg gtgtgtgtca ccaccccggc ggctggggtg
     3181 cgggagcggt gcggagccgg ctctcggctg gtgcccaagc tgactcgggt cagatgtgca
     3241 cgaccgcggg cggctctggg cgattctgga cccgccaggc gcccagaagc cactcaaagc
     3301 agagagaagg ccgcctaccc tgtgctgccc ccaagctagg atcacagctc ccacttctga
     3361 tgcccaagca tttacgattt ctcatgtata ataggatgtt tgtatttatg tatgcaggta
     3421 tttataaatc tctcatgcat gttcatagat atataactgc tacatgacgt ccatcattta
     3481 agattgctgt tggaataaag ggggaaaacc cactcgtctc agagtatgtt tcttactttt
     3541 gctccatctt ttcatttttt acttcagatc tcttagttcg acttcgtcat atgatctttt
     3601 tcttaatgga aaatagagct gcggtgtagt gatagcacac caaattccag atcccccaat
     3661 tctctctgtc ccctttcccc agcaccctgc ggccaattag gattgtaatc tgcctaaatc
     3721 tctccgttga gtgtaattta gggccagaat attttacggt attatgcaga gtttcaacac
     3781 acacagtagg actcctcatt aaatgcccag ccaggggagg gtgcccaaag taaaatgaaa
     3841 ggcgcctgca ttccctgcaa tccttaacac tttcaaggca cagaagtccc aagttaccta
     3901 cccttttgcc tcgatcgccc tctctttact gcgggctcgt ggcttcctca gtttaacctc
     3961 cttccgcccc tccgtttagt tagggtgggc ttcccctttc cagcccccac ccccacccca
     4021 ctccgcccca tacaatcagg gaggtccttg gcaagggggg gaggccaatt cccaggtgaa
     4081 cgccccctcc ggtcctatta gcctcggcct agtctattca cagcaaagag ccaatcagaa
     4141 gctcattggc cctgggcgcg ccccagcttg cgcccagcgc gccctggctc tggctccggg
     4201 cgctctcctg caccgcccct ggggcgcggg agtgcgatgc aacctgttga tgctactgtt
     4261 gcttttactt tcccagtggc tgtaatcccg aaaggagtct catcctctag gagagatgtt
     4321 aaaaaagagc ccaagagata gctccctgga atatgtcttt gcaatgtcca gaggcagttg
     4381 aatgttgttt tcgcaaacat ttgccggggc ttgatctaac ctgtcatcta gaactccttg
     4441 ggcagtagat tttctgggac tggactggac atatcctata aagatacgga atggcagaat
     4501 agggtgctaa aatgatgttt aagacattat tcttcacctg tttcttcttc aacctcgtct
     4561 gtttgctggt gtctaggaat agtcttgggc gtatcttacg tgccagaaac ttcagagctt
     4621 tattcttgtg tgaatgtttg ggctctgtgg ggatcctcta gagtcgacct gcaggcatgc
     4681 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     4861 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4921 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4981 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5041 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5101 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5281 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5341 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5401 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5461 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5521 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5581 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5641 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5701 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5761 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5821 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5881 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5941 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6001 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6061 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6301 gatcaaagga tcttc
//
