LOCUS       sequence-376491-       12414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PALI1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Zhang Q, Agius SC, Flanigan SF, Uckelmann M, Levina V, Owen BM, 
            Davidovich C
  TITLE     PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an
            allosteric activation of catalysis.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4592. doi: 10.1038/s41467-021-24866-3.
  PUBMED    34321472
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1):4592. doi"; pages: " 
            10.1038/s41467-021-24866-3"
COMMENT     Sequence Label: PALI1_pHIVEGFP
FEATURES             Location/Qualifiers
     source          1..12414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2624..3802
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2855..3793
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3750..3770
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3841..3864
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8536..8556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(8576..9150,9151..9153,9154..9162)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8743..8760)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8970..8989
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9163..9165,9166..9168,9169..9882)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9163..9165,9166..9168,9169..9879)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9208..9229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9469..9488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9816..9837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9894..9910)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9895..9911)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10420..10600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10662..11250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10742..10761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11421..12212,12213..12281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12044..12063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12282..12386)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagcg tgaggctccg gtgcccgtca
     2641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2701 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2821 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2881 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2941 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3001 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3061 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3121 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3181 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3241 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3301 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3361 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3421 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3481 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3541 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3601 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3661 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3721 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3781 tcttccattt caggtgtcgt gagcggccgc tgagttaact attctagagt acccaccatg
     3841 gactacaaag acgatgacga caagatgcag cgaatgatcc aacaatttgc tgctgaatat
     3901 acctcaaaaa atagctctac tcaggacccc agccagccca atagcacaaa gaaccaaagc
     3961 ctgccgaaag catctccagt caccacctct cccacggctg caactactca gaaccctgtg
     4021 ctcagcaaac ttctcatggc tgaccaagac tcacctctgg accttactgt cagaaagtct
     4081 cagtcagaac ctagcgaaca agacggtgta cttgatctgt ccactaagaa aagtccatgt
     4141 gctggcagca cttccctgag ccactctcca ggctgctcca gtactcaagg gaacggtgag
     4201 aactcaacag aggcaaaagc agtagattct aacaatcagt cgaagtcccc actggagaaa
     4261 tttatggtca aactgtgtac tcatcatcaa aagcaattca ttcgtgttct gaacgacctg
     4321 tacactgaat ctcaaccagg cactgaggac ctgcagcctt ctgattcggg agcaatggat
     4381 gtatccactt gcaatgctgg ctgtgcccag ctcagcacca aacataagga aaaagatgct
     4441 ctgtgtctcg atatgaagtc ttctgcttct gtagatttgt tcgtagactc gtcagactct
     4501 cacagccctc tacacttgac ggaacagacc ccgaagaagc ctcctcctga gataaaccct
     4561 gtagatggaa gagagaatgc cttgactgtt gtccagaaag attcctctga acttccaacc
     4621 actaaatcga attctattaa tagcagttca gtggatagtt tcactccggg atacctcact
     4681 gcatctaatt gttcctcagt gaacttccac cacatcccta aaatcttgga ggggcagacc
     4741 actggacaag agcaagacac aaatgtgaac atatgtgagg atggtaaaga ccatatgcag
     4801 agttcagctt tagtagaaag tctaattaca gtaaaaatgg cagctgagaa tagtgaggaa
     4861 ggcaatacct gtattattcc tcaaagaaat ttgttcaaag ctttatcaga agaggcttgg
     4921 aactcagggt ttatggggaa ctcatctaga actgctgaca aagagaatac tttacagtgt
     4981 ccaaaaacac ctttgcgcca ggatttagag gcaaatgaac aagatgcaag gccaaagcaa
     5041 gagaaccatc ttcactctct gggaagaaat aaggtgggtt accatttaca tcccagtgat
     5101 aagggccagt ttgatcattc caaagatggt tggttaggcc ccggccctat gccagctgta
     5161 cacaaagcgg caaatggaca ctcaagaacc aagatgatat caacctccat caagacagct
     5221 cggaaaagta aaagggcatc agggctgagg ataaatgatt atgataacca gtgtgatgtt
     5281 gtttatatca gtcaaccaat aacagaatgc cactttgaga atcaaaaatc aatattatct
     5341 tctcggaaaa cagccagaaa gagtactcga ggatactttt tcaatggtga ctgttgtgag
     5401 ctgccaactg ttcgtacact ggccagaaat ttacactccc aggaaaaagc aagctgctca
     5461 gcattggcat cagaggcagt tttcactcct aagcagaccc ttacaattcc agcccctaga
     5521 catacagtag atgtgcagct tcccagagaa gacaaccctg aagaacctag caaggaaatc
     5581 acctctcacg aggaaggagg tggagacgtt tcacctcgaa aagaacctca agagcctgag
     5641 gtttgcccca caaagattaa gccgaacctg agcagctccc ctaggtcaga ggaaacgaca
     5701 gcctccagcc tggtgtggcc tctccctgct caccttcctg aagaggacct gccagaaggt
     5761 ggctccacag tctcagctcc cacagcaagt gggatgtctt ctcctgaaca caaccaacca
     5821 ccagttgcac tgttggatac ggaggagatg agtgtacccc aggactgtca cctccttccc
     5881 tccactgaaa gcttttccgg gggagtcagt gaagatgtca tttctaggcc tcattctcct
     5941 cctgaaatag tcagtagaga agaaagtcct cagtgctcag aaaatcagag ttccccaatg
     6001 ggcttggagc cccccatgag tctgggaaag gctgaggaca accaaagcat cagtgctgag
     6061 gttgagtctg gagacaccca ggagctaaat gtcgacccac tcttgaagga aagcagcact
     6121 tttactgatg aaaaccccag tgaaactgag gaaagtgagg cagcaggtgg tataggaaaa
     6181 ttagagggag aggacggtga tgtaaaatgc ctgtcagaaa aagacacgta tgatacaagc
     6241 attgactcac tcgaagagaa tttggacaag aagaaaaaag gtaaaaaatt ccctgaggcc
     6301 tctgataggt gcctaagaag tcaactttcg gattcttcct ctgctgacag atgcctaaga
     6361 aatcagagtt cagattcttc ctcagcttgt cttgaaatca aagttcctaa aaatcctagt
     6421 gcaaaacgtt caaaaaaaga agggcaccct ggtgggacaa cacctaaggg ccttctacct
     6481 gacagtttcc acacggaaac tctggaggac acagaaaagc caagtgtcaa tgaacgcccc
     6541 tctgagaaag atgctgagca ggagggcgaa ggcgggggga tcatcaccag gcagactttg
     6601 aaaaacatgc tggacaaaga agtcaaggag ttacgaggag agattttccc cagcagggac
     6661 cccataacca cagctggaca gccactgcct ggagagagat tggaaatcta tgttcagtct
     6721 aaaatggatg agaagaatgc tcatatcccc tcagaaagta ttgcttgtaa gagggaccca
     6781 gaacaggcaa aagaagagcc agggcatatt cccacacagc atgtggagga ggctgtgaat
     6841 gaggtagaca acgaaaacac ccagcagaaa gatgatgaga gtgatgcccc atgcagctct
     6901 cttgggttgt cgagtagtgg aagtggtgat gctgctaggg caccaaaatc ggtgccaagg
     6961 cctaaaagat tgacctcttc aacctacaac ctaagacacg ctcattctct gggctccttg
     7021 gatgcttcaa aagtgacttc agaaaaggaa gctgcacaag taaaccccat aatgccaaag
     7081 gaaaatggag cttcagagag tggagacccc ctagatgagg acgatgttga caccgtggta
     7141 gatgaacagc caaagtttat ggaatggtgt gctgaggagg agaaccaaga gctcatcgcc
     7201 aacttcaatg cccagtacat gaaagttcag aagggctgga tccagttgga gaaagaagga
     7261 cagccaacac caagagcaag gaacaaatca gataaactga aagagatttg gaaaagcaag
     7321 aaaaggtcac ggaaatgtag gagttcattg gagagtcaga agtgttctcc tgttcagatg
     7381 ctctttatga caaactttaa attatctaat gtttgtaaat ggttcttaga gacaactgaa
     7441 acccggtctc tagtcattgt gaagaagctc aatactcgcc ttccaggaga cgttccccct
     7501 gtcaagcatc ctcttcagaa atacgctcct tccagcctat atcccagttc actacaggct
     7561 gagcgcttga aaaagcactt gaagaaattt cctggagcta cccctgctaa gaataattgg
     7621 aaaatgcaga agctctgggc caaatttcga gagaatcctg atcaagtgga gccagaagat
     7681 ggcagtgatg tcagccccgg ccctaattct gaagacagca tagaggaagt caaggaagat
     7741 agaaacagtc atcctccagc aaacctgccc actccagcca gtacccggat tcttagaaaa
     7801 tattccaata ttcgaggaaa gctcagagcc cagcaacgtt taatcaagaa tgagaaaatg
     7861 gaatgcccag atgctctggc tgtggaaagt aagccaagtc gtaagagcgt atgcatcaac
     7921 cctctgatgt cccccaagct tgccctgcaa gtggatgcag atgggtttcc tgttaagccc
     7981 aagagtactg aaggaatgaa gggaaggaag gggaagcagg tgtctgaaat cttgcctaaa
     8041 gcagaagttc agagtaaacg caagagaaca gaaggcagca gccctccaga tagtaagaac
     8101 aaggggccta cggtgaaagc cagcaaagaa aagcatgctg atggagccac caaaacccct
     8161 gctgccaaga ggccagctgc aagggacaga agcagccaac cccccaaaaa gacgtctttg
     8221 aaagagaata aagtgaagat ccctaaaaag tccgctggga agagctgccc tccctccagg
     8281 aaagaaaaag agaatacaaa caaaaggcct tcccagtcta ttgcctcgga aacactgacg
     8341 aaacctgcaa aacagaaggg ggccggtgaa tcctcttcaa ggcctcagaa agccacgaat
     8401 aggaagcaga gtagtggaaa gactcgggcc agaccctcaa cgaaaacccc agagagcagt
     8461 gcagctcaga gaaagcgaaa gctgaaggca aagctggact gttcgcacag caaacggagg
     8521 cggctggatg caaagccaaa aaagaagaga aaggtataat aggggctagg atccgcccct
     8581 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     8641 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     8701 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     8761 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     8821 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     8881 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     8941 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9001 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     9061 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     9121 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     9181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     9241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     9301 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     9361 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     9421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     9481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     9541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     9601 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     9661 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     9721 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     9781 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     9841 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg catcgatacc
     9901 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     9961 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
    10021 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10081 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
    10141 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
    10201 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
    10261 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
    10321 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
    10381 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
    10441 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10501 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10561 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
    10621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11221 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    11281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    11341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11401 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11461 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11521 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11581 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11641 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11701 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11761 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11821 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11881 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11941 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    12001 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12061 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12121 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12181 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12241 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
