LOCUS       sequence-376523-        8582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Gluc-RMA and EGFP driven by the Tet Response Element 
            (TRE). Plasmid 2 for recording Fos expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Lee S, Nouraein S, Kwon JJ, Huang Z, Wojick JA, Xia B, Corder G, 
            Szablowski JO
  TITLE     Engineered serum markers for non-invasive monitoring of gene 
            expression in the brain.
  JOURNAL   Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x.
  PUBMED    38200117
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x."
COMMENT     Sequence Label: pAAV-TRE-Gluc-RMA-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..8582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(107..126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     316..337
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        500..604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(605..673,674..1465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(823..842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1636..2224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2125..2144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2529..2753
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2820..3353)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3523..3528,3529..3545,3546..3551))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   3891..4020
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4040..4354
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4048..4066
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4084..4102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4119..4137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4155..4173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4191..4209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4226..4244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4262..4280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4327..4351
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4370..4379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4376..4930
                     /codon_start=1
                     /product="enhanced variant of secreted Gaussia luciferase"
                     /label=Gaussia-Dura Luc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGEKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIDDIPEIPGFKDLEPIEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGDD"
     CDS             4376..4930
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGEKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIDDIPEIPGFKDLEPIEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGDD"
     misc_feature    join(5596..6170,6171..6173,6174..6182)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5763..5780)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5990..6009
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6183..6185,6186..6188,6189..6899)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6183..6185,6186..6188,6189..6899)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6228..6249)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6489..6508)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6836..6857
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6909..6929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6960..7548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7013..7033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7431..7442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7575..7594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7576..7594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7620..7754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7783..7923
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7783..7912
                     /label=AAV2 ITR
     polyA_signal    8090..8224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8253..8418
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
       61 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      241 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      301 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      421 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
      481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2401 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2461 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
     2521 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     2581 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     2641 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     2701 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt
     2761 tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt
     2821 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
     2881 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
     2941 ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc
     3001 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
     3061 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
     3121 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
     3181 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
     3241 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
     3301 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     3361 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     3421 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     3481 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     3541 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     3601 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3661 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3721 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     3781 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3841 ttcgcccagg actctagcta tagttctagt ggttggctac agcttgcatg cctgcaggca
     3901 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
     3961 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
     4021 gcggccgcac gcgcgtctcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     4081 tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg
     4141 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     4201 tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta
     4261 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat
     4321 aagcagagct cgtttagtga accgtcagat cgccaccggt gtttaaacgg ccaccatggg
     4381 agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc gaggccaagc ccaccgagaa
     4441 caacgaagac ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg atctcgatgc
     4501 tgaccgcggg aagttgcccg gcgagaagct gccgctggag gtgctcaaag agttggaagc
     4561 caatgcccgg aaagctggct gcaccagggg ctgtctgatc tgcctgtccc acatcaagtg
     4621 cacgcccaag atgaagaagt tcatcccagg acgctgccac acctacgaag gcgacaaaga
     4681 gtccgcacag ggcggcatag gcgaggcgat cgacgacatt cctgagattc ctgggttcaa
     4741 ggacttggag cccatagagc agttcatcgc acaggtcgat ctgtgtgtgg actgcacaac
     4801 tggctgcctc aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga agtggctgcc
     4861 gcaacgctgc gcgacctttg ccagcaagat ccagggccag gtggacaaga tcaagggggc
     4921 cggtgatgac ggctccgtcc cagaagtatc atctgtcttc atcttccccc caaagcccaa
     4981 ggatgtgctc accattactc tgactcctaa ggtcacgtgt gttgtggtag acatcagcaa
     5041 ggatgatccc gaggtccagt tcagctggtt tgtagatgat gtggaggtgc acacagctca
     5101 gacgcaaccc cgggaggagc agttcaacag cactttccgc tcagtcagtg aacttcccat
     5161 catgcaccag gactggctca atggcaagga gttcaaatgc agggtcaaca gtgcagcttt
     5221 ccctgccccc atcgagaaaa ccatctccaa aaccaaaggc agaccgaagg ctccacaggt
     5281 gtacaccatt ccacctccca aggagcagat ggccaaggat aaagtcagtc tgacctgcat
     5341 gataacagac ttcttccctg aagacattac tgtggagtgg cagtggaatg ggcagccagc
     5401 ggagaactac aagaacactc agcccatcat ggacacagat ggctcttact tcgtctacag
     5461 caagctcaat gtgcagaaga gcaactggga ggcaggaaat actttcacct gctctgtgtt
     5521 acatgagggc ctgcacaacc accatactga gaagagcctc tcccactctc ctggtaaatg
     5581 attctagggg atccgcccct ctccctcccc cccccntaac gttactggcc gaagccgctt
     5641 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5701 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5761 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5821 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5881 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5941 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     6001 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     6061 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     6121 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     6181 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6241 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6301 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6361 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     6421 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6481 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6541 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6601 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6661 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6721 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6781 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6841 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     6901 gtggttctcc caagaagaag aggaaagtct aagtttaatt cgatatcaag cttatcgata
     6961 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7021 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7081 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7141 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7201 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7261 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7321 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7381 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7441 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7501 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     7561 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg cagcggccga ccatggccca
     7621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7741 atcatgtctg gatctccgga cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     7801 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     7861 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     7921 aggtctgaga caataaccct gataaatgct tcaataatgt aagcttgtcg agaagtacta
     7981 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     8041 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8101 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8161 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8221 gatctgatca ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa
     8281 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     8341 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     8401 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
     8461 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
     8521 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
     8581 gc
//
