LOCUS       sequence-37653-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Iioka H, Loiselle D, Haystead TA, Macara IG
  TITLE     Efficient detection of RNA-protein interactions using tethered RNAs.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(8):e53. Epub 2011 Feb 7.
  PUBMED    21300640
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(8)"; pages: "e53. Epub 
            2011 Feb 7"
COMMENT     Sequence Label: pcDNA3-tRNA scaffolded SA (tRSA)
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(310..328)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(311..328)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(348..365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    354..578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
       61 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgaa aaaaaaaaaa
      121 gcccggatag ctcagtcggt agagcagcgg cctcgaccag aatcatgcaa gtgcgtaaga
      181 tagtcgcggg tcgaggccgc gtccagggtt caagtccctg ttcgggcgcc actgcagaaa
      241 aaaaaaaaag aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
      301 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      361 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      421 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      481 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      541 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      601 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
//
