LOCUS       sequence-376536-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 M219V
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     417..434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    551..572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(587..592,593..610,611..617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    625..641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     630..652
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     649..665
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..665
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     684..704
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        686..704
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        732..958
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             959..1139
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1186..1311
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1804..2037
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2222..2266
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2497..2707
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2519..2715
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2566..2701
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2747..3349
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2747..2766)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3243..3263
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3392..3509
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3647..4825
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3878..4816
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4773..4793
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4845..4869
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6616..6640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6648..6689
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6740..7328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6793..6813)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7211..7222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7400..7633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7711..7832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7748..7767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7802..7821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7872..8007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8027..8046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8028..8046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8056..8073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8056..8072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8065..8087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8214..8669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8301..8320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8511..8532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8695..8799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8800..8868,8869..9660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9018..9037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9831..10156,1..263)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
       61 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      121 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      181 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      241 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      301 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      361 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      421 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      481 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      541 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      601 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      661 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
      721 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
      781 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
      841 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
      901 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
      961 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1021 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1081 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1141 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     1201 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     1261 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     1321 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1381 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1441 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1501 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1561 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1621 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1681 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1741 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1801 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1861 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1921 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1981 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2041 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     2101 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2161 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2221 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2281 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2341 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2401 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     2461 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2521 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2581 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2641 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2701 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     2761 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     2821 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     2881 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     2941 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3001 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3061 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     3121 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     3181 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     3241 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     3301 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
     3361 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3421 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3481 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     3541 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
     3601 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     3661 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     3721 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     3781 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     3841 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     3901 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3961 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     4021 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4081 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4141 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     4201 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     4261 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     4321 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     4381 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     4441 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     4501 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     4561 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     4621 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     4681 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     4741 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     4801 ttttcttcca tttcaggtgt cgtgaggcta gcatcgattg atcaacaagt ttgtacaaaa
     4861 aagttggcat gagtagtagt ttaggaaaag aaaaagactc taaagaaaaa gatcccaaag
     4921 taccatcagc caaggaaaga gaaaaggagg caaaagcctc tggaggtttt gggaaagaga
     4981 gcaaagaaaa agaacctaag accaaaggga aagatgccaa agatggaaag aaggactcca
     5041 gtgctgccca accaggggtg gcattttcag ttgacaatac gatcaaacgg ccaaacccag
     5101 cacctgggac tagaaaaaaa tccagcaatg cagaggtgat taaagagctc aacaaatgcc
     5161 gggaagagaa ttcaatgcgt ttggacttat ccaagagatc tatacacata ttgccatcat
     5221 caatcaaaga gttgactcaa ttaacagaac tttatttata cagtaacaaa ttgcagtccc
     5281 tcccagcaga ggtgggatgt ttagtaaatc tcatgacact ggctctaagt gaaaattcac
     5341 ttaccagttt gcctgactct cttgataact tgaagaagct gcggatgctt gatttacggc
     5401 ataataaact gagagaaatt ccttcagtgg tgtataggct ggattctctc accactcttt
     5461 atcttcgatt taatcgtata actactgtgg aaaaggacat caaaaacttg tcaaaactca
     5521 gcgtgcttag cattcgagag aacaaaatta aacaactacc tgctgaaatt ggtgaattat
     5581 gtaatctcat cacgctggat gtagctcaca atcaacttga acaccttcca aaggagattg
     5641 gaaactgtac acagataacc aaccttgact tgcagcacaa tgaactgcta gacctcccag
     5701 atactatagg aaacctgtcc agtttaagtc gtcttggtct gagatataac agactgtcag
     5761 caatacccag atcattagca aaatgcagtg cacttgaaga attaaattta gagaacaata
     5821 acatttctac tttaccagag agtcttttat caagtcttgt gaaactgaat agtttgacct
     5881 tagctagaaa ttgcttccag ttgtatccag tgggtggtcc atctcagttt tctaccatct
     5941 attccctcaa catggaacac aatcgaatca acaaaattcc atttggaatt ttctccagag
     6001 caaaagtatt aagtaagctg aatatgaagg acaatcagtt aacatcactt cccttggatt
     6061 ttggaacttg gaccagtatg gtagaattga atttagccac taatcagctc acaaagatcc
     6121 ctgaggatgt gtctggtctc gtttctcttg aggttcttat cttatcaaac aatcttctaa
     6181 agaagcttcc ccatggtctt ggaaacctta ggaagttaag agagttggat ctagaagaga
     6241 acaaattgga atccttgcca aatgaaattg catatcttaa ggatttacag aaattagtct
     6301 tgacaaacaa ccagttgacc actcttccca gaggcattgg tcaccttact aatctcacac
     6361 atctgggcct tggagagaac ctacttactc accttcctga agaaattggt acactggaga
     6421 acctagaaga actgtatttg aatgacaacc ccaacctgca tagccttccc tttgagctgg
     6481 cactctgcag caagctttca atcatgagta ttgagaactg tccactcagt caccttccac
     6541 ctcagattgt tgctgggggg ccttctttca tcattcagtt cttaaagatg cagggtccat
     6601 atcgtgccat ggtctgccca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     6661 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaccggt taagtcgaca
     6721 atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6781 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6841 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6901 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6961 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7021 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7081 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7141 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7201 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7261 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7321 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7381 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7441 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7501 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7561 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7621 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     7681 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7861 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7921 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7981 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8041 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8101 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8161 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8221 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8281 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8341 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8401 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8461 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8521 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8581 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8641 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9001 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9481 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9661 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9781 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9841 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9901 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9961 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10021 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10081 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10141 tcgggctgaa cggggg
//
