LOCUS       sequence-376537-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 R182E
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     256..277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        440..544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(545..613,614..1405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(763..782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1576..2164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2065..2084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2318..2335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2452..2473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2488..2493,2494..2511,2512..2518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2526..2542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2531..2553
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2550..2566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2550..2566
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2585..2605
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2587..2605
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2633..2859
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2860..3040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3087..3212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3705..3938
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4123..4167
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4398..4608
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4420..4616
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4467..4602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4648..5250
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(4648..4667)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5144..5164
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5293..5410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5548..6726
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5779..6717
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6674..6694
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6746..6770
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8517..8541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8549..8590
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8641..9229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8694..8714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9112..9123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9301..9534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9612..9733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9649..9668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9703..9722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9773..9908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9928..9947)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9929..9947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9957..9974)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9957..9973)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9966..9988)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10115..10156,1..414)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
       61 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      121 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      181 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      241 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      301 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      361 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
      421 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      481 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      541 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      601 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      661 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      721 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      781 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      841 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      901 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      961 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1021 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1081 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1141 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1201 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1261 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1321 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1381 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1441 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1501 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1561 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1621 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1681 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1741 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1801 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1861 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1921 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1981 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2041 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2101 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2161 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2221 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2281 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2341 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2401 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2461 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2521 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2581 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     2641 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     2701 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     2761 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     2821 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     2881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     2941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     3061 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     3121 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     3181 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     3241 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     3301 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     3361 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     3421 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     3481 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     3541 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     3601 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     3661 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     3721 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     3781 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     3841 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     3901 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     3961 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     4021 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     4081 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     4141 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     4201 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     4261 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     4321 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa
     4381 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa
     4441 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4621 tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4681 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4741 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4801 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4861 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4921 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4981 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5041 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5101 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5161 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5221 tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt
     5281 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     5341 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     5401 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggc
     5461 ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     5521 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     5581 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     5641 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5701 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5761 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     5821 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     5881 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     5941 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     6001 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     6061 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     6121 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     6181 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     6241 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     6301 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     6361 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     6421 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     6481 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     6541 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     6601 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     6661 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     6721 tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca tgagtagtag
     6781 tttaggaaaa gaaaaagact ctaaagaaaa agatcccaaa gtaccatcag ccaaggaaag
     6841 agaaaaggag gcaaaagcct ctggaggttt tgggaaagag agcaaagaaa aagaacctaa
     6901 gaccaaaggg aaagatgcca aagatggaaa gaaggactcc agtgctgccc aaccaggggt
     6961 ggcattttca gttgacaata cgatcaaacg gccaaaccca gcacctggga ctagaaaaaa
     7021 atccagcaat gcagaggtga ttaaagagct caacaaatgc cgggaagaga attcaatgcg
     7081 tttggactta tccaagagat ctatacacat attgccatca tcaatcaaag agttgactca
     7141 attaacagaa ctttatttat acagtaacaa attgcagtcc ctcccagcag aggtgggatg
     7201 tttagtaaat ctcatgacac tggctctaag tgaaaattca cttaccagtt tgcctgactc
     7261 tcttgataac ttgaagaagc tgcggatgct tgatttacgg cataataaac tggaggaaat
     7321 tccttcagtg gtgtataggc tggattctct caccactctt tatcttcgat ttaatcgtat
     7381 aactactgtg gaaaaggaca tcaaaaactt gtcaaaactc agcatgctta gcattcgaga
     7441 gaacaaaatt aaacaactac ctgctgaaat tggtgaatta tgtaatctca tcacgctgga
     7501 tgtagctcac aatcaacttg aacaccttcc aaaggagatt ggaaactgta cacagataac
     7561 caaccttgac ttgcagcaca atgaactgct agacctccca gatactatag gaaacctgtc
     7621 cagtttaagt cgtcttggtc tgagatataa cagactgtca gcaataccca gatcattagc
     7681 aaaatgcagt gcacttgaag aattaaattt agagaacaat aacatttcta ctttaccaga
     7741 gagtctttta tcaagtcttg tgaaactgaa tagtttgacc ttagctagaa attgcttcca
     7801 gttgtatcca gtgggtggtc catctcagtt ttctaccatc tattccctca acatggaaca
     7861 caatcgaatc aacaaaattc catttggaat tttctccaga gcaaaagtat taagtaagct
     7921 gaatatgaag gacaatcagt taacatcact tcccttggat tttggaactt ggaccagtat
     7981 ggtagaattg aatttagcca ctaatcagct cacaaagatc cctgaggatg tgtctggtct
     8041 cgtttctctt gaggttctta tcttatcaaa caatcttcta aagaagcttc cccatggtct
     8101 tggaaacctt aggaagttaa gagagttgga tctagaagag aacaaattgg aatccttgcc
     8161 aaatgaaatt gcatatctta aggatttaca gaaattagtc ttgacaaaca accagttgac
     8221 cactcttccc agaggcattg gtcaccttac taatctcaca catctgggcc ttggagagaa
     8281 cctacttact caccttcctg aagaaattgg tacactggag aacctagaag aactgtattt
     8341 gaatgacaac cccaacctgc atagccttcc ctttgagctg gcactctgca gcaagctttc
     8401 aatcatgagt attgagaact gtccactcag tcaccttcca cctcagattg ttgctggggg
     8461 gccttctttc atcattcagt tcttaaagat gcagggtcca tatcgtgcca tggtctgccc
     8521 aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     8581 cgattctacg tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac
     8641 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8701 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8761 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8821 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8881 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8941 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9001 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     9061 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9121 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9181 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     9241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9301 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     9541 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9601 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9721 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     9781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9841 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9901 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9961 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10021 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10081 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    10141 gcggcgggtg tggtgg
//
