LOCUS       sequence-376539-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2V5 N434D
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     414..434
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    563..680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        818..1996
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1049..1987
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1944..1964
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2016..2040
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3787..3811)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3819..3860
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3911..4499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3964..3984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4382..4393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4571..4804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4882..5003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4919..4938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4973..4992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5043..5178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5198..5217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5199..5217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5227..5244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5227..5243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5236..5258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5385..5840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5472..5491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5682..5703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5866..5970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5971..6039,6040..6831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6189..6208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7002..7590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7491..7510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7744..7761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7878..7899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7914..7919,7920..7937,7938..7944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7952..7968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7957..7979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7976..7992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7976..7992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8011..8031
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8013..8031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8059..8285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8286..8466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8513..8638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9131..9364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9549..9593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9824..10034
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9846..10042
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9893..10028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(10074..10156,1..520)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10074..10093)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
       61 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
      121 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
      181 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
      241 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
      301 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
      361 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
      421 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
      481 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
      541 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      601 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      661 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
      721 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
      781 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      841 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      901 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      961 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     1021 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     1081 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     1141 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     1201 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1261 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1321 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1381 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1441 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1501 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1561 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1621 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1681 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1741 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1801 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1861 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1921 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1981 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     2041 tgagtagtag tttaggaaaa gaaaaagact ctaaagaaaa agatcccaaa gtaccatcag
     2101 ccaaggaaag agaaaaggag gcaaaagcct ctggaggttt tgggaaagag agcaaagaaa
     2161 aagaacctaa gaccaaaggg aaagatgcca aagatggaaa gaaggactcc agtgctgccc
     2221 aaccaggggt ggcattttca gttgacaata cgatcaaacg gccaaaccca gcacctggga
     2281 ctagaaaaaa atccagcaat gcagaggtga ttaaagagct caacaaatgc cgggaagaga
     2341 attcaatgcg tttggactta tccaagagat ctatacacat attgccatca tcaatcaaag
     2401 agttgactca attaacagaa ctttatttat acagtaacaa attgcagtcc ctcccagcag
     2461 aggtgggatg tttagtaaat ctcatgacac tggctctaag tgaaaattca cttaccagtt
     2521 tgcctgactc tcttgataac ttgaagaagc tgcggatgct tgatttacgg cataataaac
     2581 tgagagaaat tccttcagtg gtgtataggc tggattctct caccactctt tatcttcgat
     2641 ttaatcgtat aactactgtg gaaaaggaca tcaaaaactt gtcaaaactc agcatgctta
     2701 gcattcgaga gaacaaaatt aaacaactac ctgctgaaat tggtgaatta tgtaatctca
     2761 tcacgctgga tgtagctcac aatcaacttg aacaccttcc aaaggagatt ggaaactgta
     2821 cacagataac caaccttgac ttgcagcaca atgaactgct agacctccca gatactatag
     2881 gaaacctgtc cagtttaagt cgtcttggtc tgagatataa cagactgtca gcaataccca
     2941 gatcattagc aaaatgcagt gcacttgaag aattaaattt agagaacaat aacatttcta
     3001 ctttaccaga gagtctttta tcaagtcttg tgaaactgaa tagtttgacc ttagctagaa
     3061 attgcttcca gttgtatcca gtgggtggtc catctcagtt ttctaccatc tattccctca
     3121 acatggaaca caatcgaatc aacaaaattc catttggaat tttctccaga gcaaaagtat
     3181 taagtaagct gaatatgaag gacaatcagt taacatcact tcccttggat tttggaactt
     3241 ggaccagtat ggtagaattg aatttagcca ctaatcagct cacaaagatc cctgaggatg
     3301 tgtctggtct cgtttctctt gaggttctta tcttatcaga taatcttcta aagaagcttc
     3361 cccatggtct tggaaacctt aggaagttaa gagagttgga tctagaagag aacaaattgg
     3421 aatccttgcc aaatgaaatt gcatatctta aggatttaca gaaattagtc ttgacaaaca
     3481 accagttgac cactcttccc agaggcattg gtcaccttac taatctcaca catctgggcc
     3541 ttggagagaa cctacttact caccttcctg aagaaattgg tacactggag aacctagaag
     3601 aactgtattt gaatgacaac cccaacctgc atagccttcc ctttgagctg gcactctgca
     3661 gcaagctttc aatcatgagt attgagaact gtccactcag tcaccttcca cctcagattg
     3721 ttgctggggg gccttctttc atcattcagt tcttaaagat gcagggtcca tatcgtgcca
     3781 tggtctgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc
     3841 tcctcggtct cgattctacg tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg
     3901 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3961 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4021 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4081 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4141 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4201 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4261 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4321 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4381 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4441 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4501 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4561 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4801 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4861 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4981 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5161 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     5221 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5281 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5341 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5401 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5461 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5521 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5581 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5641 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5701 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5761 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5821 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5881 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5941 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6001 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6061 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6121 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6181 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6241 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6301 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6361 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6421 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6481 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6541 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6601 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6661 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6721 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6781 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6841 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6901 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7681 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7741 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7801 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7861 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7921 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7981 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8041 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     8101 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     8161 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     8221 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     8281 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8341 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8401 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8461 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8521 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8581 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8641 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8701 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8761 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8821 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8881 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8941 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     9001 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     9061 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     9121 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     9181 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     9241 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     9301 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     9361 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     9421 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     9481 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     9541 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9601 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9661 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9721 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct
     9781 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9841 aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9961 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10021 tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg
    10081 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
    10141 tcgccgccgc gttcgc
//
