LOCUS       sequence-376541-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 V64G
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     376..396
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    525..642
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        780..1958
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1011..1949
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1906..1926
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1978..2002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3749..3773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3781..3822
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3873..4461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3926..3946)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4344..4355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4533..4766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4844..4965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4881..4900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4935..4954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5005..5140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5160..5179)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5161..5179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5189..5206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5189..5205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5198..5220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5347..5802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5434..5453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5644..5665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5828..5932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5933..6001,6002..6793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6151..6170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6964..7552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7453..7472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7706..7723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7840..7861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7876..7881,7882..7899,7900..7906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7914..7930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7919..7941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7938..7954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7938..7954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7973..7993
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7975..7993
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8021..8247
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8248..8428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8475..8600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9093..9326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9511..9555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9786..9996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9808..10004
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9855..9990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(10036..10156,1..482)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10036..10055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
       61 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
      121 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
      181 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
      241 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
      301 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
      361 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
      421 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt
      481 aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
      541 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      601 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      661 agcagagatc cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt
      721 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
      781 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      841 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      901 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      961 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     1021 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     1081 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     1141 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     1201 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     1261 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     1321 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     1381 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     1441 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     1501 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     1561 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     1621 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     1681 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     1741 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1801 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1861 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1921 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca
     1981 agtttgtaca aaaaagttgg catgagtagt agtttaggaa aagaaaaaga ctctaaagaa
     2041 aaagatccca aagtaccatc agccaaggaa agagaaaagg aggcaaaagc ctctggaggt
     2101 tttgggaaag agagcaaaga aaaagaacct aagaccaaag ggaaagatgc caaagatgga
     2161 aagaaggact ccagtgctgc ccaaccaggg ggcgcatttt cagttgacaa tacgatcaaa
     2221 cggccaaacc cagcacctgg gactagaaaa aaatccagca atgcagaggt gattaaagag
     2281 ctcaacaaat gccgggaaga gaattcaatg cgtttggact tatccaagag atctatacac
     2341 atattgccat catcaatcaa agagttgact caattaacag aactttattt atacagtaac
     2401 aaattgcagt ccctcccagc agaggtggga tgtttagtaa atctcatgac actggctcta
     2461 agtgaaaatt cacttaccag tttgcctgac tctcttgata acttgaagaa gctgcggatg
     2521 cttgatttac ggcataataa actgagagaa attccttcag tggtgtatag gctggattct
     2581 ctcaccactc tttatcttcg atttaatcgt ataactactg tggaaaagga catcaaaaac
     2641 ttgtcaaaac tcagcatgct tagcattcga gagaacaaaa ttaaacaact acctgctgaa
     2701 attggtgaat tatgtaatct catcacgctg gatgtagctc acaatcaact tgaacacctt
     2761 ccaaaggaga ttggaaactg tacacagata accaaccttg acttgcagca caatgaactg
     2821 ctagacctcc cagatactat aggaaacctg tccagtttaa gtcgtcttgg tctgagatat
     2881 aacagactgt cagcaatacc cagatcatta gcaaaatgca gtgcacttga agaattaaat
     2941 ttagagaaca ataacatttc tactttacca gagagtcttt tatcaagtct tgtgaaactg
     3001 aatagtttga ccttagctag aaattgcttc cagttgtatc cagtgggtgg tccatctcag
     3061 ttttctacca tctattccct caacatggaa cacaatcgaa tcaacaaaat tccatttgga
     3121 attttctcca gagcaaaagt attaagtaag ctgaatatga aggacaatca gttaacatca
     3181 cttcccttgg attttggaac ttggaccagt atggtagaat tgaatttagc cactaatcag
     3241 ctcacaaaga tccctgagga tgtgtctggt ctcgtttctc ttgaggttct tatcttatca
     3301 aacaatcttc taaagaagct tccccatggt cttggaaacc ttaggaagtt aagagagttg
     3361 gatctagaag agaacaaatt ggaatccttg ccaaatgaaa ttgcatatct taaggattta
     3421 cagaaattag tcttgacaaa caaccagttg accactcttc ccagaggcat tggtcacctt
     3481 actaatctca cacatctggg ccttggagag aacctactta ctcaccttcc tgaagaaatt
     3541 ggtacactgg agaacctaga agaactgtat ttgaatgaca accccaacct gcatagcctt
     3601 ccctttgagc tggcactctg cagcaagctt tcaatcatga gtattgagaa ctgtccactc
     3661 agtcaccttc cacctcagat tgttgctggg gggccttctt tcatcattca gttcttaaag
     3721 atgcagggtc catatcgtgc catggtctgc ccaactttct tgtacaaagt ggttgatatc
     3781 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc
     3841 ggttaagtcg acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     3901 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3961 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4021 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4081 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4141 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4201 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4261 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4321 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4381 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4441 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     4501 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4561 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4621 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4681 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4741 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     4801 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     4861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     4981 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5101 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5161 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5221 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5281 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5341 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5401 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5461 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5521 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5581 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5641 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5701 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5761 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     5821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7621 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7681 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7741 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7801 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7861 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7921 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7981 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     8041 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8101 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     8161 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8221 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8281 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8341 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8401 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8461 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8521 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8581 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8641 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8701 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8761 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8821 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8881 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8941 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9001 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9061 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9121 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9181 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9241 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9301 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9361 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9421 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9481 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9541 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9601 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9661 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9721 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9781 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
     9841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9961 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
    10021 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    10081 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    10141 accgtcgatc cggacc
//
