LOCUS       sequence-376542-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 T411A
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..237
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        49..245
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      96..231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             277..879
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(277..296)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     773..793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    922..1039
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1177..2355
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1408..2346
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2303..2323
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2375..2399
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4146..4170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4178..4219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4270..4858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4323..4343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4741..4752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4930..5163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5241..5362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5278..5297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5332..5351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5402..5537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5557..5576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5558..5576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5586..5603)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5586..5602)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5595..5617)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5744..6199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5831..5850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6041..6062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6225..6329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6330..6398,6399..7190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6548..6567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7361..7949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7850..7869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8103..8120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8237..8258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8273..8278,8279..8296,8297..8303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8311..8327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8316..8338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8335..8351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8335..8351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8370..8390
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8372..8390
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8418..8644
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8645..8825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8872..8997
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9490..9723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9908..9952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
       61 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      241 gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg
      301 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
      361 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
      421 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
      481 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
      541 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
      601 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
      661 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
      721 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
      781 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
      841 cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca
      901 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      961 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1021 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
     1081 gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     1141 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     1201 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     1261 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     1321 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     1381 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     1441 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     1501 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     1561 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     1621 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     1681 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     1741 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     1801 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1861 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1921 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1981 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     2041 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     2101 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     2161 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     2221 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     2281 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     2341 tttcaggtgt cgtgaggcta gcatcgattg atcaacaagt ttgtacaaaa aagttggcat
     2401 gagtagtagt ttaggaaaag aaaaagactc taaagaaaaa gatcccaaag taccatcagc
     2461 caaggaaaga gaaaaggagg caaaagcctc tggaggtttt gggaaagaga gcaaagaaaa
     2521 agaacctaag accaaaggga aagatgccaa agatggaaag aaggactcca gtgctgccca
     2581 accaggggtg gcattttcag ttgacaatac gatcaaacgg ccaaacccag cacctgggac
     2641 tagaaaaaaa tccagcaatg cagaggtgat taaagagctc aacaaatgcc gggaagagaa
     2701 ttcaatgcgt ttggacttat ccaagagatc tatacacata ttgccatcat caatcaaaga
     2761 gttgactcaa ttaacagaac tttatttata cagtaacaaa ttgcagtccc tcccagcaga
     2821 ggtgggatgt ttagtaaatc tcatgacact ggctctaagt gaaaattcac ttaccagttt
     2881 gcctgactct cttgataact tgaagaagct gcggatgctt gatttacggc ataataaact
     2941 gagagaaatt ccttcagtgg tgtataggct ggattctctc accactcttt atcttcgatt
     3001 taatcgtata actactgtgg aaaaggacat caaaaacttg tcaaaactca gcatgcttag
     3061 cattcgagag aacaaaatta aacaactacc tgctgaaatt ggtgaattat gtaatctcat
     3121 cacgctggat gtagctcaca atcaacttga acaccttcca aaggagattg gaaactgtac
     3181 acagataacc aaccttgact tgcagcacaa tgaactgcta gacctcccag atactatagg
     3241 aaacctgtcc agtttaagtc gtcttggtct gagatataac agactgtcag caatacccag
     3301 atcattagca aaatgcagtg cacttgaaga attaaattta gagaacaata acatttctac
     3361 tttaccagag agtcttttat caagtcttgt gaaactgaat agtttgacct tagctagaaa
     3421 ttgcttccag ttgtatccag tgggtggtcc atctcagttt tctaccatct attccctcaa
     3481 catggaacac aatcgaatca acaaaattcc atttggaatt ttctccagag caaaagtatt
     3541 aagtaagctg aatatgaagg acaatcagtt aacatcactt cccttggatt ttggaacttg
     3601 gaccagtatg gtagaattga atttagccgc taatcagctc acaaagatcc ctgaggatgt
     3661 gtctggtctc gtttctcttg aggttcttat cttatcaaac aatcttctaa agaagcttcc
     3721 ccatggtctt ggaaacctta ggaagttaag agagttggat ctagaagaga acaaattgga
     3781 atccttgcca aatgaaattg catatcttaa ggatttacag aaattagtct tgacaaacaa
     3841 ccagttgacc actcttccca gaggcattgg tcaccttact aatctcacac atctgggcct
     3901 tggagagaac ctacttactc accttcctga agaaattggt acactggaga acctagaaga
     3961 actgtatttg aatgacaacc ccaacctgca tagccttccc tttgagctgg cactctgcag
     4021 caagctttca atcatgagta ttgagaactg tccactcagt caccttccac ctcagattgt
     4081 tgctgggggg ccttctttca tcattcagtt cttaaagatg cagggtccat atcgtgccat
     4141 ggtctgccca actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct
     4201 cctcggtctc gattctacgt agtaatgaac tagtaccggt taagtcgaca atcaacgcgt
     4261 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4321 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4381 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4441 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4501 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4561 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4621 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4681 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4741 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4801 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     4861 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4921 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4981 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5041 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5101 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5161 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5221 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5341 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5401 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5461 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5521 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5581 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5641 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5701 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5941 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6001 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6061 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6121 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6181 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7021 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7201 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7261 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7501 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8161 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8221 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8281 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8341 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8401 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8461 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8521 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     8581 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8641 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8701 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8761 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8821 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8881 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8941 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9001 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9061 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9121 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9181 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9241 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9301 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9361 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9421 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9481 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9541 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9601 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9661 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9721 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9781 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9841 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9901 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9961 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10021 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10081 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
    10141 aattagtcag caacca
//
