LOCUS       sequence-376543-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 R223F
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2089..2315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2316..2496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2543..2668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3161..3394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3579..3623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3854..4064
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3876..4072
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3923..4058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4104..4706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(4104..4123)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4600..4620
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4749..4866
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5004..6182
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5235..6173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6130..6150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6202..6226
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7973..7997)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8005..8046
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8097..8685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8150..8170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8568..8579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8757..8990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9068..9189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9105..9124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9159..9178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9229..9364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9384..9403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9385..9403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9413..9430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9413..9429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9422..9444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9571..10026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9658..9677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9868..9889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10052..10156
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     2881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3781 caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc
     3841 cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat
     3901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc
     4081 tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     4141 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     4201 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     4261 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg
     4321 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     4381 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     4441 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     4501 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     4561 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     4621 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     4681 tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc
     4741 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4801 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4861 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc
     4921 aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
     4981 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     5041 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     5101 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     5161 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     5221 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     5281 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     5341 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     5401 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     5461 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     5521 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     5581 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     5641 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     5701 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     5761 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     5821 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     5881 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     5941 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     6001 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     6061 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     6121 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     6181 gaggctagca tcgattgatc aacaagtttg tacaaaaaag ttggcatgag tagtagttta
     6241 ggaaaagaaa aagactctaa agaaaaagat cccaaagtac catcagccaa ggaaagagaa
     6301 aaggaggcaa aagcctctgg aggttttggg aaagagagca aagaaaaaga acctaagacc
     6361 aaagggaaag atgccaaaga tggaaagaag gactccagtg ctgcccaacc aggggtggca
     6421 ttttcagttg acaatacgat caaacggcca aacccagcac ctgggactag aaaaaaatcc
     6481 agcaatgcag aggtgattaa agagctcaac aaatgccggg aagagaattc aatgcgtttg
     6541 gacttatcca agagatctat acacatattg ccatcatcaa tcaaagagtt gactcaatta
     6601 acagaacttt atttatacag taacaaattg cagtccctcc cagcagaggt gggatgttta
     6661 gtaaatctca tgacactggc tctaagtgaa aattcactta ccagtttgcc tgactctctt
     6721 gataacttga agaagctgcg gatgcttgat ttacggcata ataaactgag agaaattcct
     6781 tcagtggtgt ataggctgga ttctctcacc actctttatc ttcgatttaa tcgtataact
     6841 actgtggaaa aggacatcaa aaacttgtca aaactcagca tgcttagcat tttcgagaac
     6901 aaaattaaac aactacctgc tgaaattggt gaattatgta atctcatcac gctggatgta
     6961 gctcacaatc aacttgaaca ccttccaaag gagattggaa actgtacaca gataaccaac
     7021 cttgacttgc agcacaatga actgctagac ctcccagata ctataggaaa cctgtccagt
     7081 ttaagtcgtc ttggtctgag atataacaga ctgtcagcaa tacccagatc attagcaaaa
     7141 tgcagtgcac ttgaagaatt aaatttagag aacaataaca tttctacttt accagagagt
     7201 cttttatcaa gtcttgtgaa actgaatagt ttgaccttag ctagaaattg cttccagttg
     7261 tatccagtgg gtggtccatc tcagttttct accatctatt ccctcaacat ggaacacaat
     7321 cgaatcaaca aaattccatt tggaattttc tccagagcaa aagtattaag taagctgaat
     7381 atgaaggaca atcagttaac atcacttccc ttggattttg gaacttggac cagtatggta
     7441 gaattgaatt tagccactaa tcagctcaca aagatccctg aggatgtgtc tggtctcgtt
     7501 tctcttgagg ttcttatctt atcaaacaat cttctaaaga agcttcccca tggtcttgga
     7561 aaccttagga agttaagaga gttggatcta gaagagaaca aattggaatc cttgccaaat
     7621 gaaattgcat atcttaagga tttacagaaa ttagtcttga caaacaacca gttgaccact
     7681 cttcccagag gcattggtca ccttactaat ctcacacatc tgggccttgg agagaaccta
     7741 cttactcacc ttcctgaaga aattggtaca ctggagaacc tagaagaact gtatttgaat
     7801 gacaacccca acctgcatag ccttcccttt gagctggcac tctgcagcaa gctttcaatc
     7861 atgagtattg agaactgtcc actcagtcac cttccacctc agattgttgc tggggggcct
     7921 tctttcatca ttcagttctt aaagatgcag ggtccatatc gtgccatggt ctgcccaact
     7981 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     8041 tctacgtagt aatgaactag taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc
     8101 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8161 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8221 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8281 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8341 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8401 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8461 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8521 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8581 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8641 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     8701 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8761 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8821 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8881 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8941 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     9001 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     9061 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9181 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     9241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9361 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     9421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     9601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9721 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9781 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9901 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9961 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    10021 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    10081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10141 ttgaaaaagg aagagt
//
