LOCUS       sequence-376544-       10742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-/GCCACC-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..745
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(796..1370,1371..1373,1374..1382)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(963..980)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1190..1209
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1391..1400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1397..2107
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1542..1560)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1810..1829
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2120..2708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2173..2193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2591..2602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2853..2886)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2906..3086
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3111..3129)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3112..3129)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3419..3438)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3652..3671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3771..3793
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3831..3849)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3917..4021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4022..4090,4091..4882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4240..4259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5053..5641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5542..5561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5795..5812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5882..5902)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5887..6216
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6067..6202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7350..7415
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7545..7565
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7990..8124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8027..8046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8081..8100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8292..8925
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8972..9097
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9594..9827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10012..10056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10323..10440
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10497..10708
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcgagtaact aactaagcca ccatgggcga attcagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      241 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      481 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagttcga ataagcatgc atctagggcg gccaattccg
      781 cccctctccc ccccccccct ctccctcccc cccccntaac gttactggcc gaagccgctt
      841 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
      901 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
      961 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     1021 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     1081 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     1141 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     1201 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     1261 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     1321 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
     1381 cccgggatcc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     1441 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     1501 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1561 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     1621 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1681 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1741 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1801 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1861 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1921 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1981 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     2041 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     2101 caagtaagcg gccgccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2161 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2221 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2281 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2341 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2401 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2461 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     2521 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2581 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2641 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2701 ctccccgcat cgggcaattc gagctcggta cctttaagac caatgactta caaggcagct
     2761 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     2821 cgaagacaag atgggatcaa ttcaccatgg gaataacttc gtatagcata cattatacga
     2881 agttatgctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     2941 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3001 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3061 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     3121 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     3181 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     3241 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     3301 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     3361 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     3421 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     3481 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     3541 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     3601 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     3661 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3721 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3781 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3841 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4021 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4081 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4141 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4201 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4261 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4321 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4381 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4441 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4501 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4561 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4621 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4681 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4741 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4801 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4861 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4921 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5821 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5881 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5941 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6001 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6061 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6121 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6181 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     6241 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     6301 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     6361 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     6421 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     6481 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     6541 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     6601 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     6661 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     6721 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     6781 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     6841 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     6901 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     6961 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     7021 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     7081 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     7141 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     7201 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     7261 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     7321 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     7381 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     7441 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     7501 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     7561 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     7621 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     7681 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     7741 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     7801 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     7861 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     7921 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     7981 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8101 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     8161 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     8221 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     8281 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     8341 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8401 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8461 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8521 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8581 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8641 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8701 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8941 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9001 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9061 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9121 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9181 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9241 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9301 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9361 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9421 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9481 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9541 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9601 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9661 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9721 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9781 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9841 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9901 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9961 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10021 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    10081 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    10141 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    10201 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    10261 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
    10321 acttttaaaa gaaaaggggg gattgggggg gacagtgcag gggaaagaat agtagacata
    10381 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    10441 atcgatcacg agactagcct cgacaagctt gatcgatggc tccggtgccc gtcagtgggc
    10501 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
    10561 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
    10621 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
    10681 tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gcatccaggc ctaagcttac
    10741 gc
//
