LOCUS       sequence-376549-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 E457K
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..249
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        61..257
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      108..243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             289..891
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(289..308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     785..805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    934..1051
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1189..2367
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1420..2358
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2315..2335
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2387..2411
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4158..4182)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4190..4231
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4282..4870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4335..4355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4753..4764)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4942..5175
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5253..5374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5290..5309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5344..5363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5414..5549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5569..5588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5570..5588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5598..5615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5598..5614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5607..5629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5756..6211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5843..5862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6053..6074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6237..6341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6342..6410,6411..7202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6560..6579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7373..7961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7862..7881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8115..8132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8249..8270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8285..8290,8291..8308,8309..8315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8323..8339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8328..8350
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8347..8363
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8347..8363
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8382..8402
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8384..8402
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8430..8656
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8657..8837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8884..9009
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9502..9735
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9920..9964
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
       61 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
      181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
      241 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
      301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
      361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
      421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
      481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
      541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
      601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
      661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
      721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
      781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
      841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
      901 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      961 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     1021 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     1081 actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg
     1141 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     1201 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     1261 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     1321 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     1381 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     1441 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     1501 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     1561 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     1621 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     1681 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     1741 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     1801 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     1861 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     1921 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     1981 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     2041 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     2101 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     2161 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     2221 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     2281 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     2341 ttttttcttc catttcaggt gtcgtgaggc tagcatcgat tgatcaacaa gtttgtacaa
     2401 aaaagttggc atgagtagta gtttaggaaa agaaaaagac tctaaagaaa aagatcccaa
     2461 agtaccatca gccaaggaaa gagaaaagga ggcaaaagcc tctggaggtt ttgggaaaga
     2521 gagcaaagaa aaagaaccta agaccaaagg gaaagatgcc aaagatggaa agaaggactc
     2581 cagtgctgcc caaccagggg tggcattttc agttgacaat acgatcaaac ggccaaaccc
     2641 agcacctggg actagaaaaa aatccagcaa tgcagaggtg attaaagagc tcaacaaatg
     2701 ccgggaagag aattcaatgc gtttggactt atccaagaga tctatacaca tattgccatc
     2761 atcaatcaaa gagttgactc aattaacaga actttattta tacagtaaca aattgcagtc
     2821 cctcccagca gaggtgggat gtttagtaaa tctcatgaca ctggctctaa gtgaaaattc
     2881 acttaccagt ttgcctgact ctcttgataa cttgaagaag ctgcggatgc ttgatttacg
     2941 gcataataaa ctgagagaaa ttccttcagt ggtgtatagg ctggattctc tcaccactct
     3001 ttatcttcga tttaatcgta taactactgt ggaaaaggac atcaaaaact tgtcaaaact
     3061 cagcatgctt agcattcgag agaacaaaat taaacaacta cctgctgaaa ttggtgaatt
     3121 atgtaatctc atcacgctgg atgtagctca caatcaactt gaacaccttc caaaggagat
     3181 tggaaactgt acacagataa ccaaccttga cttgcagcac aatgaactgc tagacctccc
     3241 agatactata ggaaacctgt ccagtttaag tcgtcttggt ctgagatata acagactgtc
     3301 agcaataccc agatcattag caaaatgcag tgcacttgaa gaattaaatt tagagaacaa
     3361 taacatttct actttaccag agagtctttt atcaagtctt gtgaaactga atagtttgac
     3421 cttagctaga aattgcttcc agttgtatcc agtgggtggt ccatctcagt tttctaccat
     3481 ctattccctc aacatggaac acaatcgaat caacaaaatt ccatttggaa ttttctccag
     3541 agcaaaagta ttaagtaagc tgaatatgaa ggacaatcag ttaacatcac ttcccttgga
     3601 ttttggaact tggaccagta tggtagaatt gaatttagcc actaatcagc tcacaaagat
     3661 ccctgaggat gtgtctggtc tcgtttctct tgaggttctt atcttatcaa acaatcttct
     3721 aaagaagctt ccccatggtc ttggaaacct taggaagtta agagagttgg atctagaaaa
     3781 gaacaaattg gaatccttgc caaatgaaat tgcatatctt aaggatttac agaaattagt
     3841 cttgacaaac aaccagttga ccactcttcc cagaggcatt ggtcacctta ctaatctcac
     3901 acatctgggc cttggagaga acctacttac tcaccttcct gaagaaattg gtacactgga
     3961 gaacctagaa gaactgtatt tgaatgacaa ccccaacctg catagccttc cctttgagct
     4021 ggcactctgc agcaagcttt caatcatgag tattgagaac tgtccactca gtcaccttcc
     4081 acctcagatt gttgctgggg ggccttcttt catcattcag ttcttaaaga tgcagggtcc
     4141 atatcgtgcc atggtctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     4201 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtaccg gttaagtcga
     4261 caatcaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     4321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4681 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4801 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4861 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4921 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4981 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5041 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5101 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5161 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     5221 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5521 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     5581 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5641 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5701 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     5761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6181 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8221 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8281 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8341 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     8401 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     8461 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     8521 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     8581 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     8641 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8701 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8761 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8821 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8881 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8941 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9001 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9061 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9121 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9181 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9241 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9301 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     9361 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9421 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9481 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9541 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9601 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9661 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9721 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9781 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9841 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9901 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9961 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    10021 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    10081 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
    10141 gcattgcatc tcaatt
//
