LOCUS       sequence-376551-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 F66V
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             46..90
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        321..531
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        343..539
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      390..525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             571..1173
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(571..590)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1067..1087
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1216..1333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1471..2649
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1702..2640
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2597..2617
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2669..2693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4440..4464)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4472..4513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4564..5152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4617..4637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5035..5046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5224..5457
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5535..5656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5572..5591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5626..5645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5696..5831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5851..5870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5852..5870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5880..5897)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5880..5896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5889..5911)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6038..6493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6125..6144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6335..6356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6519..6623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6624..6692,6693..7484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6842..6861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7655..8243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8144..8163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8397..8414
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8531..8552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8567..8572,8573..8590,8591..8597)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8605..8621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8610..8632
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8629..8645
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8629..8645
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8664..8684
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8666..8684
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8712..8938
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8939..9119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9166..9291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9784..10017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
       61 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
      121 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
      181 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
      241 cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg
      301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag
      361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      541 agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc
      601 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
      661 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
      721 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
      781 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
      841 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
      901 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
      961 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     1021 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     1081 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     1141 tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc
     1201 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     1261 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     1321 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     1381 ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
     1441 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     1501 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     1561 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     1621 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     1681 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     1741 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     1801 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     1861 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     1921 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     1981 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     2041 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     2101 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     2161 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     2221 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     2281 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     2341 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     2401 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     2461 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     2521 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     2581 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     2641 gtgtcgtgag gctagcatcg attgatcaac aagtttgtac aaaaaagttg gcatgagtag
     2701 tagtttagga aaagaaaaag actctaaaga aaaagatccc aaagtaccat cagccaagga
     2761 aagagaaaag gaggcaaaag cctctggagg ttttgggaaa gagagcaaag aaaaagaacc
     2821 taagaccaaa gggaaagatg ccaaagatgg aaagaaggac tccagtgctg cccaaccagg
     2881 ggtggcagtg tcagttgaca atacgatcaa acggccaaac ccagcacctg ggactagaaa
     2941 aaaatccagc aatgcagagg tgattaaaga gctcaacaaa tgccgggaag agaattcaat
     3001 gcgtttggac ttatccaaga gatctataca catattgcca tcatcaatca aagagttgac
     3061 tcaattaaca gaactttatt tatacagtaa caaattgcag tccctcccag cagaggtggg
     3121 atgtttagta aatctcatga cactggctct aagtgaaaat tcacttacca gtttgcctga
     3181 ctctcttgat aacttgaaga agctgcggat gcttgattta cggcataata aactgagaga
     3241 aattccttca gtggtgtata ggctggattc tctcaccact ctttatcttc gatttaatcg
     3301 tataactact gtggaaaagg acatcaaaaa cttgtcaaaa ctcagcatgc ttagcattcg
     3361 agagaacaaa attaaacaac tacctgctga aattggtgaa ttatgtaatc tcatcacgct
     3421 ggatgtagct cacaatcaac ttgaacacct tccaaaggag attggaaact gtacacagat
     3481 aaccaacctt gacttgcagc acaatgaact gctagacctc ccagatacta taggaaacct
     3541 gtccagttta agtcgtcttg gtctgagata taacagactg tcagcaatac ccagatcatt
     3601 agcaaaatgc agtgcacttg aagaattaaa tttagagaac aataacattt ctactttacc
     3661 agagagtctt ttatcaagtc ttgtgaaact gaatagtttg accttagcta gaaattgctt
     3721 ccagttgtat ccagtgggtg gtccatctca gttttctacc atctattccc tcaacatgga
     3781 acacaatcga atcaacaaaa ttccatttgg aattttctcc agagcaaaag tattaagtaa
     3841 gctgaatatg aaggacaatc agttaacatc acttcccttg gattttggaa cttggaccag
     3901 tatggtagaa ttgaatttag ccactaatca gctcacaaag atccctgagg atgtgtctgg
     3961 tctcgtttct cttgaggttc ttatcttatc aaacaatctt ctaaagaagc ttccccatgg
     4021 tcttggaaac cttaggaagt taagagagtt ggatctagaa gagaacaaat tggaatcctt
     4081 gccaaatgaa attgcatatc ttaaggattt acagaaatta gtcttgacaa acaaccagtt
     4141 gaccactctt cccagaggca ttggtcacct tactaatctc acacatctgg gccttggaga
     4201 gaacctactt actcaccttc ctgaagaaat tggtacactg gagaacctag aagaactgta
     4261 tttgaatgac aaccccaacc tgcatagcct tccctttgag ctggcactct gcagcaagct
     4321 ttcaatcatg agtattgaga actgtccact cagtcacctt ccacctcaga ttgttgctgg
     4381 ggggccttct ttcatcattc agttcttaaa gatgcagggt ccatatcgtg ccatggtctg
     4441 cccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
     4501 tctcgattct acgtagtaat gaactagtac cggttaagtc gacaatcaac gcgttaagtc
     4561 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4921 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     5161 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5221 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5281 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5341 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5401 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5461 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5521 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5581 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5641 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5821 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5881 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5941 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6001 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6481 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6601 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6661 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6721 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6781 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6841 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6901 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6961 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7021 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7081 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7141 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7201 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7261 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7321 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7381 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7441 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8341 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8401 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8461 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8521 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8581 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8641 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8701 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     8761 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     8821 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     8881 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9181 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9241 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9301 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9361 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9421 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9481 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9541 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9601 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9661 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9721 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9781 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9841 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9901 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9961 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10021 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10081 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10141 aattaacaat tacaca
//
