LOCUS       sequence-376552-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 Q249K
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1539..1749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1561..1757
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1608..1743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1789..2391
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(1789..1808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2285..2305
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2434..2551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2689..3867
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2920..3858
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3815..3835
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3887..3911
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5658..5682)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5690..5731
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5782..6370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5835..5855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6253..6264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6442..6675
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6753..6874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6790..6809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6844..6863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6914..7049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7069..7088)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7070..7088)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7098..7115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7098..7114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7107..7129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7256..7711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7343..7362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7553..7574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7737..7841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7842..7910,7911..8702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8060..8079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8873..9461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9362..9381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9615..9632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9749..9770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9785..9790,9791..9808,9809..9815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9823..9839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9828..9850
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9847..9863
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9847..9863
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9882..9902
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9884..9902
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9930..10156
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
     1801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     1861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     1921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     1981 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     2041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     2101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     2161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     2221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     2281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     2341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
     2401 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2581 actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg
     2641 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     2701 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     2761 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     2821 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     2881 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     2941 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     3001 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     3061 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3121 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3181 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3241 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3301 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3361 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3421 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3481 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     3541 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     3601 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     3661 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3721 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3781 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3841 ttttttcttc catttcaggt gtcgtgaggc tagcatcgat tgatcaacaa gtttgtacaa
     3901 aaaagttggc atgagtagta gtttaggaaa agaaaaagac tctaaagaaa aagatcccaa
     3961 agtaccatca gccaaggaaa gagaaaagga ggcaaaagcc tctggaggtt ttgggaaaga
     4021 gagcaaagaa aaagaaccta agaccaaagg gaaagatgcc aaagatggaa agaaggactc
     4081 cagtgctgcc caaccagggg tggcattttc agttgacaat acgatcaaac ggccaaaccc
     4141 agcacctggg actagaaaaa aatccagcaa tgcagaggtg attaaagagc tcaacaaatg
     4201 ccgggaagag aattcaatgc gtttggactt atccaagaga tctatacaca tattgccatc
     4261 atcaatcaaa gagttgactc aattaacaga actttattta tacagtaaca aattgcagtc
     4321 cctcccagca gaggtgggat gtttagtaaa tctcatgaca ctggctctaa gtgaaaattc
     4381 acttaccagt ttgcctgact ctcttgataa cttgaagaag ctgcggatgc ttgatttacg
     4441 gcataataaa ctgagagaaa ttccttcagt ggtgtatagg ctggattctc tcaccactct
     4501 ttatcttcga tttaatcgta taactactgt ggaaaaggac atcaaaaact tgtcaaaact
     4561 cagcatgctt agcattcgag agaacaaaat taaacaacta cctgctgaaa ttggtgaatt
     4621 atgtaatctc atcacgctgg atgtagctca caataagctt gaacaccttc caaaggagat
     4681 tggaaactgt acacagataa ccaaccttga cttgcagcac aatgaactgc tagacctccc
     4741 agatactata ggaaacctgt ccagtttaag tcgtcttggt ctgagatata acagactgtc
     4801 agcaataccc agatcattag caaaatgcag tgcacttgaa gaattaaatt tagagaacaa
     4861 taacatttct actttaccag agagtctttt atcaagtctt gtgaaactga atagtttgac
     4921 cttagctaga aattgcttcc agttgtatcc agtgggtggt ccatctcagt tttctaccat
     4981 ctattccctc aacatggaac acaatcgaat caacaaaatt ccatttggaa ttttctccag
     5041 agcaaaagta ttaagtaagc tgaatatgaa ggacaatcag ttaacatcac ttcccttgga
     5101 ttttggaact tggaccagta tggtagaatt gaatttagcc actaatcagc tcacaaagat
     5161 ccctgaggat gtgtctggtc tcgtttctct tgaggttctt atcttatcaa acaatcttct
     5221 aaagaagctt ccccatggtc ttggaaacct taggaagtta agagagttgg atctagaaga
     5281 gaacaaattg gaatccttgc caaatgaaat tgcatatctt aaggatttac agaaattagt
     5341 cttgacaaac aaccagttga ccactcttcc cagaggcatt ggtcacctta ctaatctcac
     5401 acatctgggc cttggagaga acctacttac tcaccttcct gaagaaattg gtacactgga
     5461 gaacctagaa gaactgtatt tgaatgacaa ccccaacctg catagccttc cctttgagct
     5521 ggcactctgc agcaagcttt caatcatgag tattgagaac tgtccactca gtcaccttcc
     5581 acctcagatt gttgctgggg ggccttcttt catcattcag ttcttaaaga tgcagggtcc
     5641 atatcgtgcc atggtctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     5701 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtaccg gttaagtcga
     5761 caatcaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     5821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6361 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     6421 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6481 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6661 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     6721 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6781 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6841 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6901 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6961 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7021 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     7081 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7141 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7201 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     7261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7441 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7561 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7681 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9541 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9601 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9661 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9721 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9781 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9841 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9901 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     9961 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
    10021 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
    10081 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
    10141 agctcgatac ataaac
//
