LOCUS       sequence-376553-       10156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 WT G63R
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     389..409
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    538..655
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        793..1971
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1024..1962
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1919..1939
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1991..2015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3762..3786)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3794..3835
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3886..4474
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3939..3959)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4357..4368)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4546..4779
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4857..4978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4894..4913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4948..4967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5018..5153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5173..5192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5174..5192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5202..5219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5202..5218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5211..5233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5360..5815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5447..5466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5657..5678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5841..5945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5946..6014,6015..6806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6164..6183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6977..7565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7466..7485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7719..7736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7853..7874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7889..7894,7895..7912,7913..7919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7927..7943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7932..7954
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7951..7967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7951..7967
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7986..8006
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7988..8006
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8034..8260
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8261..8441
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8488..8613
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9106..9339
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9524..9568
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9799..10009
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9821..10017
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9868..10003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(10049..10156,1..495)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10049..10068)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
       61 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
      121 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      181 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      241 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
      301 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      361 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      421 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
      481 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
      541 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      601 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      661 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggtgcaaaga
      721 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
      781 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      841 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      901 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      961 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     1021 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     1081 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     1141 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     1201 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     1261 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     1321 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     1381 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1441 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1501 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1561 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1621 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1681 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1741 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1801 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1861 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1921 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggctagcat
     1981 cgattgatca acaagtttgt acaaaaaagt tggcatgagt agtagtttag gaaaagaaaa
     2041 agactctaaa gaaaaagatc ccaaagtacc atcagccaag gaaagagaaa aggaggcaaa
     2101 agcctctgga ggttttggga aagagagcaa agaaaaagaa cctaagacca aagggaaaga
     2161 tgccaaagat ggaaagaagg actccagtgc tgcccaacca agagtggcat tttcagttga
     2221 caatacgatc aaacggccaa acccagcacc tgggactaga aaaaaatcca gcaatgcaga
     2281 ggtgattaaa gagctcaaca aatgccggga agagaattca atgcgtttgg acttatccaa
     2341 gagatctata cacatattgc catcatcaat caaagagttg actcaattaa cagaacttta
     2401 tttatacagt aacaaattgc agtccctccc agcagaggtg ggatgtttag taaatctcat
     2461 gacactggct ctaagtgaaa attcacttac cagtttgcct gactctcttg ataacttgaa
     2521 gaagctgcgg atgcttgatt tacggcataa taaactgaga gaaattcctt cagtggtgta
     2581 taggctggat tctctcacca ctctttatct tcgatttaat cgtataacta ctgtggaaaa
     2641 ggacatcaaa aacttgtcaa aactcagcat gcttagcatt cgagagaaca aaattaaaca
     2701 actacctgct gaaattggtg aattatgtaa tctcatcacg ctggatgtag ctcacaatca
     2761 acttgaacac cttccaaagg agattggaaa ctgtacacag ataaccaacc ttgacttgca
     2821 gcacaatgaa ctgctagacc tcccagatac tataggaaac ctgtccagtt taagtcgtct
     2881 tggtctgaga tataacagac tgtcagcaat acccagatca ttagcaaaat gcagtgcact
     2941 tgaagaatta aatttagaga acaataacat ttctacttta ccagagagtc ttttatcaag
     3001 tcttgtgaaa ctgaatagtt tgaccttagc tagaaattgc ttccagttgt atccagtggg
     3061 tggtccatct cagttttcta ccatctattc cctcaacatg gaacacaatc gaatcaacaa
     3121 aattccattt ggaattttct ccagagcaaa agtattaagt aagctgaata tgaaggacaa
     3181 tcagttaaca tcacttccct tggattttgg aacttggacc agtatggtag aattgaattt
     3241 agccactaat cagctcacaa agatccctga ggatgtgtct ggtctcgttt ctcttgaggt
     3301 tcttatctta tcaaacaatc ttctaaagaa gcttccccat ggtcttggaa accttaggaa
     3361 gttaagagag ttggatctag aagagaacaa attggaatcc ttgccaaatg aaattgcata
     3421 tcttaaggat ttacagaaat tagtcttgac aaacaaccag ttgaccactc ttcccagagg
     3481 cattggtcac cttactaatc tcacacatct gggccttgga gagaacctac ttactcacct
     3541 tcctgaagaa attggtacac tggagaacct agaagaactg tatttgaatg acaaccccaa
     3601 cctgcatagc cttccctttg agctggcact ctgcagcaag ctttcaatca tgagtattga
     3661 gaactgtcca ctcagtcacc ttccacctca gattgttgct ggggggcctt ctttcatcat
     3721 tcagttctta aagatgcagg gtccatatcg tgccatggtc tgcccaactt tcttgtacaa
     3781 agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     3841 atgaactagt accggttaag tcgacaatca acgcgttaag tcgacaatca acctctggat
     3901 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3961 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4021 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4081 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4141 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4201 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4261 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4321 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4381 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4441 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     4501 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4561 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4621 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4681 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4741 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4801 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4861 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4921 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4981 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5041 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5101 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     5161 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     5221 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5281 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5341 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5401 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5461 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5521 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5581 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5641 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5701 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5761 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6181 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6481 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7801 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7861 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7921 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7981 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     8041 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     8101 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     8161 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     8221 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     8281 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8341 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8401 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8461 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8521 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8581 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8641 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8701 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8761 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8821 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8881 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8941 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     9001 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9061 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9121 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9181 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9241 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9301 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9361 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9421 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9481 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9541 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9601 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9661 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9721 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     9781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     9841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10021 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    10081 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    10141 cgccacgcgc cacacc
//
