LOCUS       sequence-37657-e         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Mihaylova MM, Vasquez DS, Ravnskjaer K, Denechaud PD, Yu RT, Alvarez
            JG, Downes M, Evans RM, Montminy M, Shaw RJ
  TITLE     Class IIa histone deacetylases are hormone-activated regulators of 
            FOXO and mammalian glucose homeostasis.
  JOURNAL   Cell. 2011 May 13;145(4):607-21.
  PUBMED    21565617
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-05-13"; volume: "145(4)"; pages: "607-21"
COMMENT     Sequence Label: pENTR/U6 HDAC4 shRNA
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(242..341)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(358..377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(359..377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(382..398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(382..398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(500..519)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccggtac aatctctctg ccaaatcgaa atttggcaga gagattgtac cttttttcta
      241 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      301 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
      361 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
      421 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      481 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
      541 ccgcgat
//
