LOCUS       sequence-376587-       11288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of cAMP sensor in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11288)
  TITLE     Expression of cAMP sensor
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Epac-SH225
FEATURES             Location/Qualifiers
     source          1..11288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2184..2205)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(2340..2852,2853..2855,2856..2858))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     2533..2552
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2792..2813
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(2869..2893)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      3701..4156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3788..3807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3998..4019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4186..4202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4186..4202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4199..4221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4210..4226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4234..4240,4241..4258,4259..4264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4279..4300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4417..4434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4588..5176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4668..4687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5347..6138,6139..6207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5970..5989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6208..6312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6380..6398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6423..6638
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6639..7442
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      7553..8899
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     9320..9342
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9334..9351
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9335..9351
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(9363..9550)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     9471..9495
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     9698..9717
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9698..9716
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9779..9803
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9812..11242)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cgctggttgt caatgacctt cagctgctgg acataagccc aggtgctggc tggactccgg
       61 gtgctcaggg actgctccga gcatgtggaa atcctcgcca cctggctgtc ctcgtggagg
      121 tgggaaactc ggcttctgag tggtgagaga ggcacagggt tgtggcttcg gcagtggtgc
      181 agcatccgcg cggctctggc catcattctc atcttctcaa agttgatgag attctccact
      241 agtgtgtggt ttccctcatg aatggcggcc atgtctttga gaagaagggg catgaagggg
      301 atgacaggag gggagagctt ggcgagggcc agtcggtata cccggtggtt ccatgaggga
      361 tccagcagcc tctcgagggc ggagtacagc ttccggactt tgtggggcag ccgctcccag
      421 gtgtgggcta ggcggctgat ggccgagttg ctgaggccaa acatgacggc aaagaaggaa
      481 ttgagattct tctgctcctt gaggtgggcc gccagcttaa tgaacttcct gagcagctgg
      541 gcccgggggc cgggcacggg gcagagacac agctcggtgg ccacccagta ctgcagctca
      601 ttgaagcggc gcatgaagcg ctccaggttg gcggtggtga catcccgcag atgctggggg
      661 cccagcacat agtggatcag ctccacctgg tggatactgt tgaagaggct ccagtcgtgg
      721 tccgtcagct ggcctgccag gtccttggca ctcaccaggt ccagcccctc agcagagccc
      781 acagtgggcc ccagctggtc agggtgtggg atcagctcat gcgcttcctg tgggttgaca
      841 acaaagagac gctcattgag ccccagagat gtggccacac cacgggcatc tggctgcagg
      901 ccaatggcat cacctgcaga attgaccttc accagcacct gccccttggt ccagccatcc
      961 tcctgggcca acgctgccat cacctctctc acggaggctg tcacaggcag ctgcagggtc
     1021 aacactgagt ggtctggccg gcagatgtca taggggactt tatccccaac ttggatggca
     1081 cagctgctgc caggaagggg ctcgtcctgg ttggggagcc aaacaggcaa gttccgggcc
     1141 ttcatctgag gagatgcatt cccacagcca ttctccaacc tgtggcatcg ccgcctctct
     1201 ggccactgct ccctcagcag gttgctgagt cgggtgtccc tgcccaccag gtctgagagt
     1261 ttctggagga agctggtggc cacagggtca gtgtggagca tggagccata cagggccacc
     1321 cactggctga ccagccgcaa gatctgctgc ctcttgttgc agacgtaggt gctgcgctcc
     1381 tgctcgctgc cacccgcagg ctccacatgg aagtggtgca gaagggcagc gcagagttgg
     1441 gcgctgggca tgaagaccct gtgggtcagg aggaagtcgc tgaggaatgt ctctgttggg
     1501 tcatgagcac tggaatctgg tcccatggcc tccaacagaa gctctaggat cttctctggg
     1561 gtgccagaca tcactgtata ccggttcctg cctggggttg ggggtcggga agggccggcg
     1621 ccctgagagg ctctctccag caccagcacc actttgccat gttcttccag ccgcatggtc
     1681 tttgcctcca catccttgat gatacggttg aagtcctgct tgtccacacg caggaaatga
     1741 cagttgtctt ctcgcaggat gatggtggct gcccggggtg catcattcac cagagccagc
     1801 tgtccaaaat catctccctc atgcagggtg gtcaccagcc ccttgccatg ggtcaccacg
     1861 ttgacagatc ccttccagat aatgtaccac gaagtgccct tgtccccctg gctgaacaac
     1921 acggtccctg ccttgctgtg tggttcaaag agcagaacag ccgctaattc tcgcttcacc
     1981 gagttggaga ggtgggccac agccttgatg tgcagcagct cctcaaagat gaggtccagc
     2041 tcttcatccg tgcgctgacc tgggggcttt cgaagtgcca cagtgagcag ggcgtcaggc
     2101 ccccgctggg agagcagggc cacagcttcg gccaactcct cctccatctc atgagttccc
     2161 acgggctgat atcgggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     2221 ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
     2281 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc
     2341 tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg
     2401 atatagacgt tgtcgctaaa gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     2461 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     2521 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     2581 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     2641 gcgaagcact gcacgcccca ggacagggtg gtcacgaggg tgggccaggg cacgggcagc
     2701 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
     2761 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     2821 accaccccgg tgaacagctc ctcgcccttg ctcaccattg cggctagcag cctgcttttt
     2881 tgtacaaact tgtgatggcc gcgaattcac tagtcgaggc tggggtatat tttttttctt
     2941 tcttttgaat gtgtgtataa aagagagaaa aaatggttat tttccaatta attaattatt
     3001 aacgattgtt aaagaaaaaa tttatcaacg aggttgatag aaaaaaaaag tttttgtaga
     3061 tatagaagaa aaaaagattt tcactattgg tgttatagga aagaaagaga aaaaatttaa
     3121 gagaaattag atgttagacg ataatgatag gacatttgaa aataatacaa tatttggcct
     3181 ttttatataa tcgaaactaa tggaggggag cataagcggt acccaccatc gaccacgttt
     3241 cgtcaccggt cataattgga ataaacaaaa caaacaaacg aactaaagag tgtgagagta
     3301 acaatttcca cagaggtaaa aaaaaaaaaa aggaaaacag aaacaaaaaa agctcatagt
     3361 tctactatag cttcttcaga ctttctgtga acgagggaaa aagacaacaa aaaagcaatg
     3421 gataaacggt ctcgatcgag gcctggaagt gccgcattag gaaatacaga tgcaatgcgc
     3481 gcgagtttac gaaattctcg caggagtttc tccggccatg tgcggctggt tctacgtctc
     3541 gtgtatgggt ttatttctgc ggtaattatc gccggatctc cgcttcgaga ccgggtttgt
     3601 tttcaaatta ccggaaaagg tcaggcaaaa cagagtgatg tatgggtgat cccagcaatg
     3661 cctgtttatg tttctccgtt tcaggaagct tggcgaatgg acgcgccctg tagcggcgca
     3721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3961 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4021 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4081 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4141 ttaacgctta caatttcctg atgcggtaag cttggcgtaa tcatggtcat agctgtttcc
     4201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4321 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4381 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5221 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5281 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5341 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5401 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5461 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5521 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5581 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5641 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5701 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5761 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5821 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5881 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5941 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6001 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6061 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6121 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6181 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6241 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6301 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6361 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattagc
     6421 ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct tgaaattttt
     6481 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     6541 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     6601 tgcacagaac aaaaacatgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     6661 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     6721 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     6781 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     6841 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     6901 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     6961 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     7021 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     7081 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     7141 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     7201 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     7261 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     7321 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     7381 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     7441 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     7501 aattatatca gttattaccc aattctcatg tttgacagct tatcatcgga tcgatccaat
     7561 atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat
     7621 agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat
     7681 atcacaggag gtactagact acctttcatc ctacataaat agacgcatat aagtacgcat
     7741 ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc
     7801 agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga
     7861 agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagctag
     7921 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca
     7981 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca
     8041 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc
     8101 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg
     8161 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga
     8221 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga
     8281 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca
     8341 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa
     8401 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt
     8461 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca
     8521 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat
     8581 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag
     8641 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc
     8701 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag
     8761 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc
     8821 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat
     8881 gaagcacaga tgcttcgtta acaaagatat gctattgaag tgcaagatgg aaacgcagaa
     8941 aatgaaccgg ggatgcgacg tgcaagatta cctatgcaat agatgcaata gtttctccag
     9001 gaaccgaaat acatacattg tcttccgtaa agcgctagac tatatattat tatacaggtt
     9061 caaatatact atctgtttca gggaaaactc ccaggttcgg atgttcaaaa ttcaatgatg
     9121 ggtaacaagt acgatccgat tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9181 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9241 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9301 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattggca
     9361 tgccggtaga ggtgtggtca ataagagcga cctcatgcta tacctgagaa agcaacctga
     9421 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt
     9481 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata
     9541 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca
     9601 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca
     9661 aacctctggc gaagaagtcc aaagctggat ccgcgcgtaa tacgactcac tatagggcga
     9721 attgggtacc gggccccccc tcgaggtcga cctgcagcgg ccatcatgaa ttaactacac
     9781 cactttgtac aagaaagctg ggtgcggccg cttacttgta cagctcgtcc atgccgtaca
     9841 ggaacaggtg gtggcggccc tcggagcgct cgtactgttc cacgatggtg tagtcctcgt
     9901 tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta gccgggcagt tgcacgggct
     9961 tcttggccat gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca
    10021 gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg
    10081 cctcccagcc catggtcttc ttctgcatta cggggccgtc gggggggaag ttggtgccgc
    10141 gcatcttcac cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca
    10201 ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
    10261 gcttcttgta atcggggatg tcggcggggt gcttcacgta cgccttggag ccgtacatga
    10321 actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct
    10381 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
    10441 ggccgttcat ggagccctcc atgcgcacct tgaagcgcat gaactctttg atgacggcca
    10501 tgttgttgtc ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccggtgccat
    10561 gccccaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt
    10621 cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagttgca
    10681 cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca
    10741 gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg
    10801 tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg
    10861 tgccgcgcat cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca
    10921 cggtcaccag accgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
    10981 aggacagctt cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt
    11041 acatgaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
    11101 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
    11161 actcgtggcc gttcatggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga
    11221 cctcctcgcc cttgctcacc atgagctcgc ttgctggctc cagctctcgg gagaggcggg
    11281 agagttcc
//
