LOCUS       sequence-376591-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) 
            with NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Sekiguchi R, Mehlferber MM, Matsumoto K, Wang S
  TITLE     Efficient Gene Knockout in Salivary Gland Epithelial Explant 
            Cultures.
  JOURNAL   J Dent Res. 2023 Feb;102(2):197-206. doi: 10.1177/00220345221128201.
            Epub 2022 Nov 10.
  PUBMED    36366748
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1177/00220345221128201"; journalName: "J Dent Res"; date: 
            "2023-02"; volume: "102"; issue: "2"; pages: "197-206"
COMMENT     Sequence Label: 
            pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..234
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     189..209
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             252..432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    479..604
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1097..1330
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1515..1559
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1857..1974
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2025..2273
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2025..2045
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2196..2215
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2295..2370
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2432..2643
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2671..2718
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2719..3411
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2849..2867)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3117..3136
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3421..3477
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3478..3876
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3892..4480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3945..3965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4363..4374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4552..4785
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4811..4828)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4817..5041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5087..5515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5174..5193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5384..5405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5524..5544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5529..5858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5709..5844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5771..5790
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5906..5953
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5972..6346
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6476..6597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6513..6532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6567..6586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6646..6662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6646..6662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6659..6681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6670..6686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6694..6700,6701..6718,6719..6724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6739..6760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6877..6894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7048..7636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7128..7147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7807..8598,8599..8667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8430..8449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8668..8772)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8847..8866)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9038..9383,1..34)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
       61 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      121 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      181 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
      241 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      301 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      361 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      421 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      481 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      541 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      601 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
      661 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
      721 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
      781 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
      841 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
      901 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
      961 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1021 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1081 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1141 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1201 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1261 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1321 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1381 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1441 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1501 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1561 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1621 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1681 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1741 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1801 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     1861 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1921 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1981 tattacaggg acagcagaga tccagtttgg ttaattaagg taccgagggc ctatttccca
     2041 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     2101 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     2161 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     2221 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgaagcag
     2281 ggccaaattg tggggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2341 caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa
     2401 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2461 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     2521 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2581 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2641 caggaccggt tctagagcgc tgccaccatg aagcgacctg ccgccacaaa gaaggctgga
     2701 caggctaaga agaagaaagt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     2761 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     2821 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     2881 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcatcaag
     2941 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     3001 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     3061 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     3121 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     3181 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg
     3241 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     3301 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     3361 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatccggc
     3421 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     3481 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     3541 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     3601 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     3661 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     3721 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     3781 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     3841 gaattgctgc cctctggtta tgtgtgggag ggctaaacgc gttaagtcga caatcaacct
     3901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4261 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4441 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4561 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4621 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4681 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4741 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     4801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5041 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5101 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5161 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5221 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5281 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5341 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5401 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5461 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5521 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5581 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5701 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5761 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5821 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5881 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     5941 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     6001 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     6061 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     6121 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     6181 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     6241 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     6301 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     6361 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6421 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6481 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6541 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6601 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6661 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7801 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7861 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7921 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7981 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8041 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8101 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8161 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8221 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8281 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8341 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8401 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8461 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8521 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8581 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8641 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8701 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8761 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     8821 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8881 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8941 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     9001 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     9061 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     9121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     9181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     9241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     9301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     9361 ttatgggact ttcctacttg gca
//
