LOCUS       sequence-37660-e         549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Mihaylova MM, Vasquez DS, Ravnskjaer K, Denechaud PD, Yu RT, Alvarez
            JG, Downes M, Evans RM, Montminy M, Shaw RJ
  TITLE     Class IIa histone deacetylases are hormone-activated regulators of 
            FOXO and mammalian glucose homeostasis.
  JOURNAL   Cell. 2011 May 13;145(4):607-21.
  PUBMED    21565617
REFERENCE   2  (bases 1 to 549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-05-13"; volume: "145(4)"; pages: "607-21"
COMMENT     Sequence Label: pENTR/U6 HDAC5 shRNA
FEATURES             Location/Qualifiers
     source          1..549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(255..354)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(371..390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(372..390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(395..411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(513..532)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgg ctcagacagg tgagaaagac gaatctttct cacctgtctg
      241 agcctttttt ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt
      301 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
      361 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
      421 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
      481 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
      541 ggaaacgtc
//
