LOCUS       sequence-376600-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HDR donor for inserting FOXL2-T2A-tdTomato reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pHDR-FOXL2-T2A-tdTomato-PuroTK
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2229..2282
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2283..3713
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    3714..3745
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        3795..4294
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3830..3853)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4227..4246
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4308..4317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4314..4913
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4813..4833
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4920..5915
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(5937..5954)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5943..6167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6215..6246
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             7492..8064
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          8141..8206
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     rep_origin      complement(8489..9077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8569..8588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgac cggtcgcaca gtcaaggagc cagaagggcc gccgccgagc ccaggcaagg
     1201 gcggtggggg tggcggcggg acagccccgg agaagccgga cccggcgcag aagcccccgt
     1261 actcgtacgt ggcgctcatc gccatggcga tccgcgagag cgcggagaag aggctcacgc
     1321 tgtccggcat ctaccagtac atcatcgcga agttcccgtt ctacgagaag aataagaagg
     1381 gctggcaaaa tagcatccgc cacaacctca gcctcaacga gtgcttcatc aaggtgccgc
     1441 gcgagggcgg cggcgagcgc aagggcaact actggacgct ggacccggcc tgcgaagaca
     1501 tgttcgagaa gggcaactac cggcgccgcc gccgcatgaa gaggcccttc cggccgccgc
     1561 ccgcgcactt ccagcccggc aaggggctct tcggggccgg aggcgccgca ggcgggtgcg
     1621 gcgtggcggg cgccggggcc gacggctacg gctacctggc gccccccaag tacctgcagt
     1681 ctggcttcct caacaactcg tggccgctac cgcagcctcc ctcacccatg ccctatgcct
     1741 cctgccagat ggcggcagcc gcagcggctg cagcagctgc ggctgcagcc gcgggccccg
     1801 gtagccctgg cgcggccgct gtggtcaagg ggctggcggg cccggccgcc tcgtacgggc
     1861 cgtacacacg cgtgcagagc atggcgctgc cccccggcgt agtgaactcg tacaatggcc
     1921 tgggaggccc gccggccgca cccccgcctc cgccgcaccc ccacccgcat ccgcacgcac
     1981 accatctgca cgcggccgcc gcaccgccgc ctgccccacc gcaccacggg gccgccgcgc
     2041 cgccgccggg ccagctcagc cctgccagcc cagccaccgc cgcgcccccg gcgcccgcgc
     2101 ccaccagtgc gccgggcctg cagttcgctt gtgcccggca gcccgagctc gccatgatgc
     2161 attgctctta ctgggaccac gacagcaaga ccggcgcgct gcattcgcgc ctcgatctcg
     2221 gctctggcga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     2281 caatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     2341 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2401 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     2461 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     2521 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2581 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     2641 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     2701 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     2761 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     2821 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     2881 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     2941 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     3001 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     3061 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     3121 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     3181 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     3241 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     3301 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     3361 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     3421 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     3481 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     3541 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     3601 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     3661 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taataacttt
     3721 aaataatgcc aattatttaa agttacatat gagaaagtat aggaacttca gcttgatatc
     3781 gaattaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     3841 agcagccccg ctgggcactt ggcgctacac aagtggcctc tgccctcgca cacattccac
     3901 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     3961 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     4021 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     4081 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     4141 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     4201 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     4261 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaacgcc accatgggga
     4321 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     4381 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     4441 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4501 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4561 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4621 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4681 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4741 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     4801 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     4861 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggatcca
     4921 tgcccacgct actgcgggtt tatatagacg gtcctcacgg gatggggaaa accaccacca
     4981 cgcaactgct ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga
     5041 cttactggca ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc
     5101 gcctcgacca gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga
     5161 taacaatggg catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg
     5221 aggctgggag ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca
     5281 tcgccgccct cctgtgctac ccggccgcgc gataccttat gggcagcatg accccccagg
     5341 ccgtgctggc gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg
     5401 gggcccttcc ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc
     5461 ggcttgacct ggctatgctg gccgcgattc gccgcgttta cgggctgctt gccaatacgg
     5521 tgcggtatct gcagggcggc gggtcgtggc gggaggattg gggacagctt tcggggacgg
     5581 ccgtgccgcc ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg
     5641 acacgttatt taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtaca
     5701 acgtgtttgc ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta
     5761 tcctggatta cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg
     5821 ggatggtcca gacccacgtc accacccccg gctccatacc gacgatctgc gacctggcgc
     5881 gcacgtttgc ccgggagatg ggggaggcta actgagctct agagctcgct gatcagcctc
     5941 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6001 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6061 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6121 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6181 aagaaccagc tggggctcga catactagag tacttaactt taaataatgc caattattta
     6241 aagttaggcg cgccgatatc tagcaccgca tgccggtgca gacggatgcg aggatgcagg
     6301 gacgcgcgac gccggccccg gtcgcagccg acgacgccgc cgccagcctg acctcacacc
     6361 ctctgggccc gcctctggag ccagcgccca gggtccctct gtgctttttc gctttcctaa
     6421 gctcctgtcg ctcctctttg tcccctcagt ttatgtcctc ctgtgctcac ctccctgacc
     6481 tctgtgacct tgcactcccc tggcctgaag ctgcctctct gcgcgctttc tactgggctc
     6541 gtctctttcc ggagccccag cgtctcctgc ccaaattcac cgcggaaagg gcccggggcg
     6601 gaggtgcgac cgggcgtcgg cagcgcagac ctcttggcct tctctcacag gtcggtgcgc
     6661 tcgctctccg cgttccccgc ccgactgccg tgcagtccat ggctagacgc gccggacagg
     6721 actgatggcg ggaccgcgct gcccgagaaa gggacggacc aatacgtgtg tttgctccgc
     6781 gaaccctctt gaagctgttc agaagccgct tgccgcgggg cccactaggc ggggcggggg
     6841 ttgggaccca gcgggagccg gggcagcctg gctccacggc ctgtactcgg tttacaccgc
     6901 gggcgggcgc ggagggaggc tgcgtttcct ccgctatcag tcccgtcgct tcgggcacct
     6961 ccgggccccg gcggctggct aatgttttgt ttgaaagatc ggtggaactt tttaagagag
     7021 tatttaaaaa aaaaaaaagg aaaaaaaatt caccgggcaa ccggggaagt attgtggcct
     7081 tggagtttgc taaatccaaa tatgaaaatc aaaagcttta gtattcctca tcttctcttc
     7141 tggaagattt gcgttagagt ttttgttggg ccttcaaaaa gctgtgttca cctccagatc
     7201 ttgtcagtgt ggttttcaag aggaagcaaa aagcctctcc acccaggcct ggaatgtttc
     7261 cacccaatgt cgagcagtgt ggttttgcaa gaggaagcaa aaagcctctc cacccaggcc
     7321 tggaatgttt ccacccaatg tcgagcaaac cccgcccagc gtcttgtcat tggcgaattc
     7381 gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac
     7441 gcgtgtggcc tcgaacaccg agcgaccctg caggtcctcg ccatggatcc tgatgatgtt
     7501 gttgattctt ctaaatcttt tgtgatggaa aacttttctt cgtaccacgg gactaaacct
     7561 ggttatgtag attccattca aaaaggtata caaaagccaa aatctggtac acaaggaaat
     7621 tatgacgatg attggaaagg gttttatagt accgacaata aatacgacgc tgcgggatac
     7681 tctgtagata atgaaaaccc gctctctgga aaagctggag gcgtggtcaa agtgacgtat
     7741 ccaggactga cgaaggttct cgcactaaaa gtggataatg ccgaaactat taagaaagag
     7801 ttaggtttaa gtctcactga accgttgatg gagcaagtcg gaacggaaga gtttatcaaa
     7861 aggttcggtg atggtgcttc gcgtgtagtg ctcagccttc ccttcgctga ggggagttct
     7921 agcgttgaat atattaataa ctgggaacag gcgaaagcgt taagcgtaga acttgagatt
     7981 aattttgaaa cccgtggaaa acgtggccaa gatgcgatgt atgagtatat ggctcaagcc
     8041 tgtgcaggaa atcgtgtcag gcgatctctt tgtgaaggaa ccttacttct gtggtgtgac
     8101 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     8161 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     8221 aactgatgaa tgggagcagt ggtggaatgc agatccacta gagtcgaggg atgcagaaat
     8281 tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt
     8341 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg
     8401 tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct
     8461 ttatgataat gtttcatagt tggatgtttt tccataggct ccgcccccct gacgagcatc
     8521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9061 gaaaaaaagg atctcaa
//
