LOCUS       sequence-376605-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) 
            with NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Sekiguchi R, Mehlferber MM, Matsumoto K, Wang S
  TITLE     Efficient Gene Knockout in Salivary Gland Epithelial Explant 
            Cultures.
  JOURNAL   J Dent Res. 2023 Feb;102(2):197-206. doi: 10.1177/00220345221128201.
            Epub 2022 Nov 10.
  PUBMED    36366748
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1177/00220345221128201"; journalName: "J Dent Res"; date: 
            "2023-02"; volume: "102"; issue: "2"; pages: "197-206"
COMMENT     Sequence Label: 
            pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..170
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             213..393
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    440..565
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1058..1291
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1476..1520
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1818..1935
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1986..2234
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1986..2006
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2157..2176
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2256..2331
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2393..2604
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2632..2679
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2680..3372
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2810..2828)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3078..3097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3382..3438
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3439..3837
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3853..4441
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3906..3926)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4324..4335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4513..4746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4772..4789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4778..5002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5048..5476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5135..5154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5345..5366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5485..5505)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5490..5819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5670..5805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5732..5751
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5867..5914
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5933..6307
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6437..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6474..6493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6528..6547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6607..6623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6607..6623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6620..6642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6631..6647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6655..6661,6662..6679,6680..6685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6700..6721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6838..6855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7009..7597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7089..7108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7768..8559,8560..8628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8391..8410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8629..8733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8808..8827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8999..9378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9380..9383,1..195)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
       61 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      121 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      181 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
      241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      361 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      421 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      481 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      541 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      601 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      661 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      721 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
      781 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
      841 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
      901 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
      961 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1021 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1081 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1141 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1201 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1261 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1321 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1381 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1441 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1501 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1561 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1621 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1681 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1741 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
     1801 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     1861 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     1921 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttaattaag
     1981 gtaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     2041 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     2101 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     2161 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2221 aaggacgaaa caccgtgctg gcgctggtgg ccgcggtttt agagctagaa atagcaagtt
     2281 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa
     2341 ttcgctagct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     2401 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     2461 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2521 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2581 aacgggtttg ccgccagaac acaggaccgg ttctagagcg ctgccaccat gaagcgacct
     2641 gccgccacaa agaaggctgg acaggctaag aagaagaaag tgagcaaggg cgaggcagtg
     2701 atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc
     2761 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2821 gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac
     2881 ggctccaggg ccttcatcaa gcaccccgcc gacatccccg actactataa gcagtccttc
     2941 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg
     3001 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc
     3061 aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc
     3121 gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg
     3181 aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg
     3241 cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac
     3301 tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac
     3361 gagctgtaca agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc
     3421 gaagagaatc ctggaccgat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     3481 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     3541 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     3601 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     3661 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     3721 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     3781 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaacg
     3841 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3901 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3961 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4021 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4081 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4141 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4201 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4261 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4321 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4381 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4441 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4501 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4561 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4621 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4681 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4741 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4801 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4861 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4921 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4981 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5041 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5461 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5521 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5641 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5701 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5761 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5821 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     5881 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     5941 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     6001 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     6061 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     6121 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     6181 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     6241 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     6301 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6361 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6421 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6541 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8761 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     8821 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     8881 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     8941 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     9001 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     9061 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9361 gtcatcgcta ttaccatggt gat
//
