LOCUS       sequence-376611-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express -Eco2 RT and ncRNA in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Lopez SC, Crawford KD, Lear SK, Bhattarai-Kline S, Shipman SL
  TITLE     Precise genome editing across kingdoms of life using retron-derived 
            DNA.
  JOURNAL   Nat Chem Biol. 2022 Feb;18(2):199-206. doi: 
            10.1038/s41589-021-00927-y. Epub 2021 Dec 23.
  PUBMED    34949838
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00927-y"; journalName: "Nat Chem Biol"; date: 
            "2022-02"; volume: "18"; issue: "2"; pages: "199-206"
COMMENT     Sequence Label: pKDC.015
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3747..3766)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3791..3839
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4070..4281
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4294..4562
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4596..5339
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5340..5393
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5394..5993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5394..5413)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5890..5910
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6026..6147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6063..6082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6117..6136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6910..6944
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7435..7451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7435..7451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7448..7470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7459..7475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7483..7489,7490..7507,7508..7513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7528..7549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7666..7683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7837..8425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7917..7936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8596..9387,9388..9456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9219..9238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagccac catgacaaaa acttcaaagc
     1801 tggatgcgct gcgggcggct actagtaggg aagatttggc gaagattctc gacataaagt
     1861 tggtgtttct gacaaacgtg ttgtaccgca taggatccga caaccagtat acgcaattca
     1921 caatacccaa aaagggtaaa ggtgtccgca ccatcagcgc accaacggac cgacttaagg
     1981 atatacagag gaggatttgt gatcttctta gtgactgtag ggatgaaatc tttgcgatta
     2041 ggaagatctc taataattac tcattcggct tcgaaagagg aaaatcaatt atactcaatg
     2101 cttacaagca tcgagggaag caaattatat tgaacatcga ccttaaggac ttctttgaga
     2161 gctttaactt tgggagagtc cgggggtact ttctctccaa ccaggacttc ttgttgaacc
     2221 cagttgtggc aacaacgttg gcgaaggccg cctgctacaa cgggactctg cctcaggggt
     2281 ccccatgttc ccctattata agtaacctta tctgtaacat tatggacatg cggctcgcaa
     2341 agctcgccaa gaagtacggc tgcacttata gtcgatatgc ggatgacatt acgatcagca
     2401 ccaataaaaa taccttcccg ttggagatgg cgactgtgca gcctgaaggg gttgtgctgg
     2461 gcaaagtgct cgtaaaggag attgaaaatt caggtttcga gattaacgat tctaagacta
     2521 gattgaccta caaaacaagt aggcaagaag tcaccgggct gacggttaat cggattgtaa
     2581 acattgatcg gtgctactac aaaaagacga gggcgctggc tcacgcattg tatcggacag
     2641 gagaatataa ggtcccagac gagaacggtg ttctggtatc tggagggctt gacaagttgg
     2701 agggtatgtt tgggtttatc gaccaggtgg ataaattcaa caacattaaa aaaaagttga
     2761 ataagcaacc cgacagatat gttctgacaa atgccacttt gcacggattt aagctcaaat
     2821 tgaacgccag ggagaaagcc tatagcaaat tcatctacta caaattcttc cacggtaata
     2881 cttgtcccac gatcataaca gagggtaaga cggataggat ttaccttaaa gctgccctcc
     2941 atagcctcga gacaagttat cctgaactgt ttcgggagaa aacagatagt aagaagaagg
     3001 agataaatct gaatattttt aaaagcaatg agaagaccaa gtatttcctg gatctcagcg
     3061 gcggcacagc agacctcaag aaattcgtgg aacgctacaa aaataactac gcttcctatt
     3121 acggcagcgt accgaaacaa ccggtgataa tggtgcttga taacgacaca ggcccgtcag
     3181 acctgttgaa ctttttgaga aacaaagtta agagttgtcc agatgatgta acagaaatgc
     3241 gcaagatgaa gtacatacat gtgttttaca atctgtacat agttctgact cccctgtctc
     3301 catctggaga gcaaacgtct atggaggacc tctttcctaa agatatattg gacattaaga
     3361 tagatggcaa gaaattcaat aaaaacaatg acggtgactc caaaacagag tatgggaagc
     3421 acatattctc aatgcgcgtt gtacgagata aaaagaggaa gatagatttc aaggcatttt
     3481 gctgtatctt cgatgctatt aaggatatta aagaacatta caaactgatg ttgaattcct
     3541 aggcggccgc gggcatttaa ctatgataag attccgtaca cgcatgtagg cagatttgtt
     3601 ggttgtgaat cgcaaccagt ggccttaatg gcaggaggaa tcgcctccct aaaatccttg
     3661 attcagagct atacggcagg tgtgctgtgc gaaggagtgc ctgcatgcgt accggttagt
     3721 aatgagttta aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     3781 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     3841 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     3901 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     3961 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgaaatcg
     4021 gatccagctg aatctaagtg gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4081 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4141 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4201 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4261 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4321 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4381 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4441 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4501 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4561 tctaagctgt gaccggcgcc tactctagag ccgccatgtc tagactggac aagagcaaag
     4621 tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     4681 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     4741 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc
     4801 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     4861 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     4921 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     4981 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     5041 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     5101 ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     5161 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     5221 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     5281 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggg
     5341 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg
     5401 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5461 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5521 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5581 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5641 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5701 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5761 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5821 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5881 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5941 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac
     6001 ctctggatta caaaatttgt gggtaaactt gtttattgca gcttataatg gttacaaata
     6061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6121 tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt
     6181 agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt
     6241 taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca
     6301 gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc
     6361 ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc
     6421 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     6481 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     6541 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     6601 tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata
     6661 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     6721 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     6781 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     6841 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     6901 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc
     6961 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     7021 gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     7081 ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga
     7141 ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg
     7201 ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca
     7261 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     7321 gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga
     7381 aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata
     7441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9421 gggaataagg gcgacacgga aatgttgaat actcat
//
